| Project/Area Number |
13470403
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
NAKASHIMA Akifumi Kyushu University Faculty of Dental Science, Assistant Professor, 大学院・歯学研究院, 助手 (20207773)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Takehisa Kyushu University Faculty of Medical Science, Professor, 大学院・歯学研究院, 教授 (60142189)
WATANABE Takeshi Department of Medicine, Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (40028684)
AKAMINE Akifumi Kyushu University Faculty of Dental Science, Professor, 大学院・歯学研究院, 教授 (00117053)
HIRATA Masako Kyushu University Dental Hospital, Assistant Professor, 歯学部附属病院, 助手 (10153769)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥13,400,000 (Direct Cost: ¥13,400,000)
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| Keywords | dental pulp stem cells / odontoblasts / dentin regeneration / gene therapy / dental pulp capping / reparative dentin / GDF11 / BMP11 / real-time RT-PCR / 象牙質・歯髄再生 / Gdf11 / Bmp11 / Dentin Sialoprotein / BMPs |
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| Research Abstract |
In order to develop a successful ex vivo gene therapy to enhance healing potential of pulp tissue and induce reparative dentin formation after injury such as caries, we developed a three-dimensional pellet culture system of pulp stem cells electrotransfected with growth/differentiation factor 11 (GDF11). The viability after electroporation was more than 85%, and the efficiency was around 65% calculated by flow cytometry. Real time RT-PCR analysis showed that the expressions of alkaline phosphatase, Dentin matrix protein 1 and Dentin sialoprotein, a differentiation marker of odontoblasts were increased in the pEGFP-Gdf11 transfected pellet compared with those in the pEGFP transfected pellet. The autogenous trasplantation of Gdf11 transfected pellet on the canine amputated pulp stimulated the reparative dentin formation during pulpal wound healing. Therefore, the ex vivo gene therapy using this pellet culture system has the potential for regeneration of exposed pulp with a large amount of dentin as an endodontic treatment
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