In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines.
| Project/Area Number |
13470441
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
NOMA Hiroyasu TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFESSOR, 歯学部, 教授 (40085791)
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| Co-Investigator(Kenkyū-buntansha) |
UZAWA Katuhiro CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISYANT PROFESSOR, 医学部, 助教授 (30302558)
TANZAWA Hideki CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (50236775)
SHIBAHARA Takahiko TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, ASSISTANT PROFESSOR, 歯学部, 助教授 (50178919)
SEKI Naohiko CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISYANT PROFESSOR, 医学部, 助教授
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥9,600,000 (Direct Cost: ¥9,600,000)
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| Keywords | cDNA microarray / oral cancer / gene expression / cDNAアイクロアレイ |
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| Research Abstract |
To analyze gene expression in oral cancer, we produced a specialized in-house cDNA microarray. The eDNA library was constructed from surgical specimens of oral squamous cell carcinoma (SCC) using an oligo-capping method. CDNA clones (n=4,800) were randomly selected and their 5'-end nucleotide sequences were determainde. Overlapping clones were excluded, and 1,423 independent clones were full-length. By correlating expression patterns across Sec cell lines, we identified 53 genes (7 up-regulated and 46 down regulated) that are differentially expressed in SCC cell lines compared to normal mucosa. Semi-quantitative RT-PCR analysis confirmed these findings and validity of our cDNA microarray. Using speciments from 5CC patient, we investigated the expression status of the IL-ira gene, which showed the down-regulated gene by microarray analysis. Gene expression clearly fell in the 5CC specimens relative to their references, which indicated that our in-house cDNA microarray system rapidly identified and characterized candidate biomarkers for clinical use.
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Report
(4 results)
Research Products
(2 results)
