| Project/Area Number |
13470464
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KURIHARA Hidemi Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (40161765)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBA Hideki Hiroshima Univ. Graduate Sch. Biomed. Sci., Researcn Associate., 大学院・医歯薬学総合研究科, 助手 (60260668)
KAWAGUCHI Hiroyuki Hiroshima Univ. Graduate Sch. Biomed. Sci., Associate Prof., 大学院・医歯薬学総合研究科, 助教授 (10224750)
KATO Yukio Hiroshima Univ. Graduate Sch. Biomed. Sci., Professor, 大学院・医歯薬学総合研究科, 教授 (10112062)
NAKAMURA Shigeo Hiroshima Univ. Graduate Sch. Biomed. Sci., Research Associate., 大学院・医歯薬学総合研究科, 助手 (00325200)
YOSHINO Hiroshi Hiroshima Unv. Dent. Hospital, Assistant Prof., 歯学部附属病院, 講師 (50240338)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2002: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2001: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | mesenchymal stem cell / pluripotent / regenerative therapy / experimental periodontal defect / periodontal regeneration / collagen gel / tissue engineering / bone marrow / 多分化能 / 歯周組織再生療法 / コラーゲンゲル / 骨髄液 |
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| Research Abstract |
Some studies have shown that mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells are useful for autologous cell transplantation. In this study, we examined usefulness for MSC transplantation into the periodontal defect. Bone marrow MSC were isolated from beagle dogs and expanded in vitro. The expanded MSC were mixed with 2% atelocollagen in various concentration (0, 2x10^6, 5x10^6, 1x10^7, 2x10^7 cells/ml) and auto-transplanted to experimental class III defects. Periodontal tissue healing was evaluated both histological and morphometrical analyses at one month after transplantation. The defects were almost completely regenerated with alveolar bone, periodontal ligament and cementum in 2x10^7 cells/ml group. Morphometrical analysis revealed that newly formed bone and cementum rates in the 2x10^7 cells/ml group was significantly higher than that in 0 cells/ml group (p<0.01). These results indicate that the auto-transplantation of bone marrow MSC might become a useful option for periodontal tissue regeneration.
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