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Redox regulation of cell adhesion signals

Research Project

Project/Area Number 13470491
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionShowa University

Principal Investigator

NOSE Kiyoshi  SHOWA UNIV., SCH.PHARM.SCI., PROFESSOR, 薬学部, 教授 (70012747)

Co-Investigator(Kenkyū-buntansha) MORI Kazunori  SHOWA UNIV., SCH.PHARM.SCI., RESEARCH ASSOCIATE, 薬学部, 助手 (60349040)
KIM-KANEYAMA Joori  SHOWA UNIV., SCH.PHARM.SCI., RESEARCH ASSOCIATE, 薬学部, 助手 (10338535)
SHIBANUMA Makoto  SHOWA UNIV., SCH.PHARM.SCI., ASSOCIATE PROFESSOR, 薬学部, 助教授 (60245876)
江川 清  昭和大学, 薬学部, 講師 (00095879)
真下 順一  昭和大学, 薬学部, 講師 (60054045)
西谷 直之  昭和大学, 薬学部, 助手 (10286867)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2003: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsHic-5 / paxillin / nuclear lozalization / c-fos / focal adhesion / 核マトリクス / 核移行 / 細胞接着斑 / パキシリン / 接着斑 / MMP / p38 / 上皮細胞
Research Abstract

Hic-5 (hydrogen peroxide-inducible clone-5) encodes a LIM protein which localizes in the focal adhesions and participates in the signal transduction from the cell surface. The Hic-5 protein translocates from the cell surface to the nucleus under oxidative condition, and regulates several target genes such as c-fos and p21. In case of c-fos genes, the transcriptional regulatory domain located at 5'-upstream region of approximately 1.5 kb from the cap site was responsible for transactivation by Hic-5. This domain contains several cis-acting sequences, but Sp1 elements was critical for the response to Hic-5. As for the mechanism of nuclear translocation of Hic-5, the LIM domains were found to be necessary. Overexpression of a cytoplasmic factor that interacts with the LIM domain 3 of Hic-5 (PTP-PEST) decreased nuclear accumulation of Hic-5 under oxidative condition. Hic-5 has an activity to oligomerize through the LIM 4 domain, and this domain functions to direct Hic-5 to the nuclear matrix fraction. Oxidative stress increased the association of Hic-5 with the nuclear matrix. These results indicate that the focal adhesion protein Hic-5 involves in cell surface signalings as well as transcriptional regulation under oxidative conditions.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(27 results)

All Other

All Publications

  • [Publications] Shibanuma, M., et al.: "A LIM protein, Hic-5, functions as a potential coactivator"J.Cell.Biochem.. 91. 633-645 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibanuma, M., et al.: "Hic-5 communicates between focal adhesions and nucleus"Mol.Biol.Cell. 14. 1158-1171 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hasebe, Y., et al.: "Specific inhibition of HIF-1α activation"Biol.Pharm.Bullet.. 26. 1379-1383 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamazaki, Y., et al.: "HIF-1-dependent VEGF reporter gene assay"Biol.Pharm.Bullet.. 26. 417-420 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim-kaneyama, S., et al.: "Transcriptional activation of the c-fos gene by---"Biochem.Biophys.Res.Commun.. 299. 360-365 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibanuma, M., Kim-Kaneyama, J., Sato, S., Nose, K.: "A LIM protein, Hic-5, functions as a potential coactivator for Sp1."J.Cell.Biochem.. 91. 633-645 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibanuma, M., Kim-Kaneyama, J., Sato, S., Ishino, K., Sakamoto, N., Mashimo, J., Nose, K.: "Hic-5 communicates between focal adhesions and the nucleus through oxidant-sensitive nuclear export signal."Mol.Biol.Cell. 14. 1158-1171 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamazaki, Y., Egawa, K., Nose, K., Kunimoto, S., Takeuchi, T.: "Hif-1-dependent VEGF reporter gene assay by a stable transformant of CHO cells"Biol.Pharm.Bullet.. 26. 417-420 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hasebe, H., Egawa, K., Yamazaki, Y., Kunimoto, S., Hirai, Y., Ida, Y., Nose K.: "Specific Inhibition of HIIF-1? Activation and of VEGF Production by Flavonoids."Biol.Pharm.Bullet.. 26. 1379-1383 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim-Kaneyama, J., Shibanuma, M., Nose, K.: "Transcriptional activation of the c-fos gene by a LIM protein, Hic-5."Biochem.Biophys.Res.Commun.. 299. 360-365 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawada, M., Usami, I., Ohba, S., Someno, T., Lim, J.W., Hayakawa, Y, Nose, K., Ishizuka, M.: "Hygrolidin induces p21 expression and abrogates cell cycle progression at G1 and S phases."Biochem.Biophys.Res.Commun.. 298. 178-183 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishiya, N., Shirai, T., Suzuki, W., Nose, K.: "Hic-5 interacts with GIT1 with a different binding mode from paxillin."J.Biochem.(Tokyo). 132. 279-289 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurihara, Y., Egawa, K., Kunimoto, S., Takeuchi, T., Nose, K.: "Induction of p16/INK4a gene expression and cellular senescence by toyocamycin."Biol.Pharm.Bullet.. 25. 1272-1276 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibanuma, M., Iwabuchi, Y., Nose, K.: "Possible involvement of Hic-5, a focal adhesion protein, in the differentiation of C2C12 myoblasts"Cell Stru.Functions. 27. 21-27 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Egawa, K., Kurihara, Y, Ito, T., Matsumoto, M., Nose, K.: "Induction of pl6INK4a transcription and of cellular senescence by aclacinomycin-derivatives and cardiac glycosides."Biol.Pharm.Bullet.. 25. 461-465 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishiya, N., Tachibana, K., Shibanuma, M., Mashimo, J., Nose K.: "Hic-5 reduced cell spreading on fibronectin: Competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase."Mol.Cell.Biol.. 21. 5332-5345 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibanuma, M., Ishino, K., Sakamoto, N., Nose, K.: "Accumulation of focal adhesion protein Hic-5 in the nucleus by hydrogen peroxide."Acta Histochem.Cytochem.. 34. 259-264 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jia, Y., Ranson, R.F., Shibanuma, M., Liu, C., Welsh, M.J., Smoyer, W.E.: "Identification and characterization of Hic-5/ARA55 as an Hsp27 binding protein."J.Biol.Chem.. 275. 39911-39918 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibanuma, M., et al.: "A LIM protein, Hic-5, functions as a potential coactivator"J.Cell.Biochem.. 91・3. 633-645 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shibanuma, M., et al.: "Hic-5 communicates between focal adhesions and nucleus"Mol.Biol.Cell.. 14・3. 1158-1171 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hasebe, Y., et al.: "Specific inhibition of HIF-1α activation and of vascular endothelial growth factor (VEGF) production by flavonoids"Biol.Pharm.Bullet.. 26・10. 1379-1383 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamazaki, Y., et al.: "HIF-1-dependent VEGF reporter gene assay by a stable transformant of CHO cells"Biol.Pharm.Bullet.. 26・4. 417-420 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shibanuma M., et al.: "Hic-5 communicates between focal adhesions and the nucleus through oxidant-sensitive nuclear export signal"Mol.Biol.Cell. 14(3)(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kim-Kaneyama, et al.: "Transcriptional activation of the c-fos gene by a LIM protein, Hic-5"Biochem.Biophys.Res.Commun.. 299. 360-365 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishiya N., et al.: "Hic-5 interacts with GIT-1 with a different binding mode from paxillin"J.Biochem.. 132. 279-289 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shibanuma.M., Ishino.K. et al.: "Accumulation of focal adhesion Protein Hic-5 in the nucleus by hydrogen peloxide"Acta Histochem. Cytochem.. 34(4). 259-264 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishiya.N., et al.: "Hic-5 reduced cell spreading on fibronectin :"Mol. Cell. Biol.. 21(16). 5332-5345 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-03-31   Modified: 2016-04-21  

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