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An effective analysis of individual cells using flow cytometry and its clinical applicatjion.

Research Project

Project/Area Number 13470516
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionThe University of Tokyo

Principal Investigator

NAKAHARA Kazuhiko  The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (70101095)

Co-Investigator(Kenkyū-buntansha) YONEYAMA Akiko  The University of Tokyo, Faculty of Medicine, Lecturer, 大学院・医学系研究科, 寄附講座教員 (50175684)
HIGASHI Katsumi  The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部附属病院, 助手 (50159109)
WATANABE Takashi  Kyorin University, Faculty of Medicine, Associate Professor, 医学部, 教授 (00191768)
IKEDA Tadako  Kyorin University, Faculty of Medicine, Lecturer, 医学部, 講師 (90099242)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥8,900,000 (Direct Cost: ¥8,900,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsflow cytometry / cell surface antigen / antigen molecule density / cell size / FF index / quantification of cell surface molecule / hematological malignancy / clinical application / 抗原密度 / 解析ソフト / リストモード / 急性白血病 / 伝染性単核球症 / list mode date / 標準ビーズ
Research Abstract

The purpose of this study is to explore an effective application of flow cytometry (FCM) to clinical medicine by using the information of individual cells. Until now, cytometric immunophenotyping using FCM has focused mainly on the quantification of the proportion of cells bearing certain combinations of molecules and the change of fluorescence intensity (FI) dose not reflect the antigen density directly. For the qualification of cell surface molecule density, the forward light scatter (FSC) and FI, obtained by FCM on an individual cell basis were plotted in a scattergram where x-and y-axis show FSC and FI respectively. Where a proportional relationship between FI and FSC can been seen, the slope of the line of regression (or its absolute value) may be used as an indicator of the molecule density, which is referred to as the FF index. In this way, the FI vs FSC scattergram enables the evaluation of the relationship between the size of the cell and the amount of antigen expressed. Additionally we explored the computer software which enables to practice automatically these analysis by using the listmode of FCM. We analyzed normal peripheral lymphocytes, hematological malignant cells and lymphocytes of hemodyalisis patients and found the potent usefulness of more precise clinical analysis using this software. In future, more clinical studies, the evaluation and standardization of the developed software and actual quantification of cell surface antigens are needed.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (32 results)

All 2003 2002 2001 Other

All Journal Article (11 results) Book (1 results) Publications (20 results)

  • [Journal Article] フローサイトメトリーによる造血器腫瘍の細胞表面抗原定量化の試み2003

    • Author(s)
      中原一彦, 米山彰子, 東克巳
    • Journal Title

      臨床病理 51補

      Pages: 261-261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 血液細胞のFI/FSC解析法の開発とその臨床応用に関する検討2003

    • Author(s)
      中原一彦, 渡邊卓
    • Journal Title

      Cytometory Research 13sup

      Pages: 24-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Quantification analysis of surface antigens on hematological malignant cells by using flow cytometry.2003

    • Author(s)
      Nakahara.K, Yoneyama A, Higashi K
    • Journal Title

      Rinsho Byori 51sup

      Pages: 261-261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The study on the development of FI/FSC analysis of hematological cells and its clinical application.2003

    • Author(s)
      Nakahara K, Watanabe T
    • Journal Title

      Cytometory Research 13sup

      Pages: 24-24

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Standard laboratory hematology.2003

    • Author(s)
      Nakahara K
    • Journal Title

      Hematology (Edited by Japanese Society of Laboratory, Ishiyaku Publisher, Inc.)

      Pages: 38-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Semi-quantification of cell surface molecule density by flow cytometry.2002

    • Author(s)
      Watanabe T, Nakahara K et al.
    • Journal Title

      Clin.Chem.Lab.Med. 40sup

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Semi-quantification of cell surface molecule density by flow cytometry.2002

    • Author(s)
      Watanabe T, Nakahara K et al.
    • Journal Title

      Clin. Chem. Lab. Med. 40sup

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] フローサイトメトリーによる細胞表面抗原の定量的解析に関する検討2001

    • Author(s)
      松島早月, 池田忠子, 中原一彦, 渡邊卓
    • Journal Title

      Cytometry Research 11sup

      Pages: 86-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 血球検査 2.フローサイトメトリー2001

    • Author(s)
      中原一彦
    • Journal Title

      臨床検査Yearbook2001 115号

      Pages: 9-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The evaluation of quantification analysis of cell surface antigens by using flow cytometry.2001

    • Author(s)
      Matsushima S, Ikeda T, Nakahara K, Watanabe T
    • Journal Title

      Cytometiy Research 11sup

      Pages: 86-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Hematological testing, 2.Flow cytometry.2001

    • Author(s)
      Nakahara K
    • Journal Title

      Rinsho Kensa Yearbook 2001 115

      Pages: 9-29

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Book] スタンダード検査血液学(日本検査血液学会編集)2003

    • Author(s)
      中原一彦
    • Publisher
      医歯薬出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中原一彦, 米山彰子, 東克巳: "フローサイトメトリーによる造血器腫瘍の細胞表面抗原定量化の試み"臨床病理. 51補. 261 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中原一彦, 渡邊卓: "血液細胞のFI/FSC解析法の開発とその臨床応用に関する検討"Cytometory Research. 13sup. 24 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中原一彦, 米山彰子, 東克巳: "造血器悪性腫瘍細胞におけるCD52抗原の発現の検討"臨床血液. 44・8. 328 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 東克巳: "フローサイトメトリー"日本検査血液会雑誌. 4・1. 150-152 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 米山彰子, 東克巳: "蛍光免疫染色"生物試料分析. 26・5. 403-413 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 東克巳: "フローサイトメトリー"Medical Technology. 32・1. 58-63 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中原一彦: "スタンダード検査血液学"医歯薬出版株式会社 日本検査血液学会編集. 38-40 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Watanabe, K.Nakahara, et al.: "Semi-quantification of cell surface molecule density by flow cytometry"Clin. Chem. Lab. Med.. 40 sup. s76 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Nakahara, et al.: "Quantification of minimal residual disease in patients with ela2 BCR-ABL-positive acute lymphoblastic leukemia using a real-time RT-PCR assay"Leukemia. 16. 1167-1175 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 米山彰子, 中原一彦: "リンパ球サブセット"透析患者の検査値の読み方. 229-230 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 米山彰子: "フローサイトメトリーのコツと落とし穴 蛍光抗体と色素"Molecular Medicine. 39. 349-356 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 東 克巳: "フローサイトメトリーによる幹細胞同定"臨床病理レビュー特集. 122号. 134-139 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 東 克巳: "フローサイトメーターの基礎と臨床"Medical Technology. 30. 659-664 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Nakahara, K.Higashi, et al.: "Global Standardization and Advanced Quality Management '01"Eibun Press, Ltd., Osaka, Japan. 48 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松島早月, 池田忠子, 中原一彦, 渡邊卓: "フローサイトメトリーによる細胞表面抗原の定量的解析に関する検討"Cytometry Research. 11sup. 86 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中原一彦: "キーとなる臨床検査 フローサイトメトリー検査"現代医療. 33(7). 1857-1864 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中原一彦: "血球検査 2フローサイトメトリー"臨床検査Yearbook 2001. 特集115. 9-29 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Togo, K.Nakahara: "Genetic analysis of phytosterolaemia"J.Inherit.Metab.Dis.. 24. 43-50 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Sakatani, K.Nakahara: "Early Relapse After High-Dose Chemotherap Resuced by Tumore-Free Autologous Peripheral Blood Stem Cells in Acute Lymphoblastic Leukemia"Leakemia and Lymphoma. 42(1-2). 225-229 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Hashimoto, K.Nakahara: "Improvement of Asthma After Administration of Pioglitazone"Diabetes Care. 25(2). 401 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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