| Project/Area Number |
13480191
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | RIKEN(The Institute of Physical and Chmical Resarch) |
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Principal Investigator |
ITO Yukishige RIKEN, Synthetic Cellular Chemistry Laboratory, Chief Scientist, 伊藤細胞制御化学研究室, 主任研究員 (80168385)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Ichiro RIKEN, Synthetic Cellular Chemistry Laboratory, Senior Resaehc Scientist, 伊藤細胞制御化学研究室, 研究員 (40342852)
MANABE Shino RIKEN, Synthetic Cellular Chemistry Laboratory, Senior Resaehc Scientist, 伊藤細胞制御化学研究室, 研究員 (60300901)
ISHIWATA Akihiro RIKEN, Synthetic Cellular Chemistry Laboratory, Resaehc Scientist, 伊藤細胞制御化学研究室, 研究員 (70342748)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2003: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | glycoprotein / quality control / chaperone / asparagine-linked / gglycan chain / stereoselctive synthesis / glycopeptide / C-mannosyl tryptophan / 立体選択性 / シアル酸 / 超高圧 / 分子シャペロン / アスパラギン酸フルオリド |
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| Research Abstract |
The aim of this study was to develop methodologies for the synthesis of glycoprotein glycans and related molecular probes. The following results were obtained.
1. Synthesis of asparagines-linked glycoprotein glycans: Proposed ligand structure of ER chaperones calnexin and calreticulin was synthesized. Analysis of specific interaction was investigated. Octamarnnrosylated glycans, which are hypothesized to be involved n the recognition of EDEM/Htm1p were synthesized.
2. Methodologies for efficient synthesis of glycpeptide Novel methods for the construction of oligosaccharide-asparagine linkage, chenroselctive peptide bond formation using thioester, and peptide elongation on sold support were developed.
3. Synthesis of C-mannnosyl tryptophan (CMW) and its derivatives: Various types of CMW-containing glycopeptides, as well as glucose analogue of CMW were synthesized.
4. Methods for the facile synthesis of oligosaccharide: New types of linkers, methods for the real-time monitoring of polymer-support oligosacharide synthesis, and capture release purification methods were developed.
5. Synthesis of enzyme inhibitors: Bisubstrate-type derivatives of sialyltransferases were synthesized systematically, and their activities were examined.
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