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Basdicmolecularmechanisms for tissue and organ formaiton: HH and FGF-dependent and regulation of positional information and compartment formation

Research Project

Project/Area Number 13480244
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionThe University of Tokyo

Principal Investigator

SAIGO Kaoru  The University of Tokyo, Department of Biophysics and Biochemistry, Graduate School of Science Professor, 大学院・理学系研究科, 教授 (50136454)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2001: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsHedgehog / FGF / Branchless / Drosophila / Wingless / visceral mesoderm / Breathless / pxb / 位置情報 / 区画化 / Bar / ホメオボックス遺伝子 / aristaless / Ras / 肢 / HH / Branchless / Wingless / Breathless / トラキア / 気管 / フィードバック
Research Abstract

In the present study, we examined basic molecular mechanisms of development using compound eyes, trachea, legs and visceral mesoderm in Drosophila as model systems. In compound eyes, reiterative use of Spitz signals emanating from already differentiated ommatidial cells triggers the differentiation of around ten different types of cells. We showed evidence that the choice of cell fate by newly recruited ommatidial cells strictly depends on their developmental potential and that developmental potential formation is governed by region-specific prepattern gene expression. Spatial y and temporally regulated activity of Breathless~ranchless signaling is essential for trachea development. We showed that late btl expression is a target of Branchless/Breathless signaling and hence, Branchless/Breathless signaling contains a positive feedback loop, which may guarantee a continuous supply of fresh receptors to membranes of growing tracheal branch cells. We also showed that hi g hI evels of HH si … More gnaling activity are essential for medial-region patterning in legs. In mid-to-late third instar lag discs, high levels of HH signals repress the transcription of pxb, a newly identified gene encoding as transmembrane protein expressed specifically in the anterior compartment. Misexpression experiments indicated that Pxb may serve as a HH signaling attenuator capable of acting prior to HH-Ptc interactions, suggesting tha HH signaling in leg discs includes a pxb-repression-mediated positive feedback loop, run visceral mesoderm gives rise to circular visceral muscles. It has been demonstrated that the trunk visceral mesoderm parasegment is subdivided into at least two domains by connectin expression. We extended this by examining a greater number of visceral mesodermal genes including HH and FGF. Ectodermal HH and Wg differentially regulate the expression of the metameric targets in trunck visceral mesoderm. HH expression in PS3 appeared required for normal Dpp expression and gastric caesum formation. Branchless(FGF) in Vmw as found to serve as a guide for the initial budding of tracheal visceral branches. The metameric pattern of Vm, organized in response to ectodermal instructive signals, is thus maintained at a later time via autoregu I ation, is required for midgut morphogenesis and exerts feedback effect on trachea, ectodermal derivatives. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(20 results)

All Other

All Publications

  • [Publications] Hayashi, T.: "Diversification of cell types in the Drosophila eye by differential expression of prepattern genes"Mech.Dev.. 108. 13-27 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshiro, T.: "Ligand-dependent activation of breathless FGF receptor gene in Drosophila developing trachea"Mech.Dev.. 114. 3-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inak, M.: "Requirements of high levels of Hedgehog signaling activity for medial-region cell fate determination in Drosophila legs : identification of pxb, a putative Hedgehog signaling attenuator gene repressed along the anterior-posterior compartment boundary"Mech.Dev.. 116. 3-18 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosono, C.: "Functional subdivision of trunk visceral mesoderm parasegments in Drosophila is required for gut and trachea development"Development. 130. 439-449 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takemae, H.: "Proteoglycan UDP-galactose:β-xylose β1,4-galactosyltransferase I is essential for viability in Drosophila melanogaster"J.Biol.Chem.. 278. 15571-15578 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamiyama, S.: "Molecular cloning and identification of 3'-phosphoadenosine 5'-phospho-sulfate transporter"J.Biol.Chem.. 278. 25958-25963 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayashi, T., Saigo, K.: "Diversification of cell types in the Drosophila eye by differential expression of prepattern genes"Mech. Dev.. 108. 13-27 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshiro, T., Emori, F., Saigo, K.: "Ligand-dependent activation of breathless FGF Receptor gene in Drosophila developing trachea"Mech. Dev.. 114. 3-11 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inaki, M., Kojima, T., Ueda, R., Saigo, K., Saigo K.: "Requirements of high levels of Hedgehog signaling activity for medial-region cell fate determination in Drosophila legs: identification of pxb, a putative Hedgehog signaling attenuator gene repressed along the anterior-posterior compartment boundary"Mech. Dev.. 116. 3-18 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosono, C., Takaira, K., Matsuda, R., Saigo, K.: "Functional subdivision of trunk visceral mesoderm parasegments in Drosophila requierd for gut and trachea development"Development. 130. 439-449 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takemae, H., Ueda, R., Okubo, R., Nakato, H., Izumi, S., Saigo, K., Nishihara, S.: "Proteoglycan UDP-galactose:β-xylose β 1.4-galacto-syltransferase I is essential for viability in Drosophila melanoagster"J. Biol. Chem.. 278. 15571-15578 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamiyama, S., Suda, T., Ueda, R., Suzuki, M., Okubo, R., Kikuchi, N., Chiba, Y., Goto, S., Toyoda, H., Saigo, K., Watanabe, M., Narimatsu, H., Jigami, Y., Nishihara, S.: "Molecular cloning and identification of 3'-phospho-Adenosine 5' -phosphosulfate transporter"J. Biol. Chem.. 278. 25958-25963 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamiyama, S.: "Molecular cloning and identification of 3'-phosphoadenosine 5'phospho-sulfate transporter"J.Biol.Chem.. 278. 25958-25963 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takemae, H.: "Regulation of metameric gene expression in developing Drosophila visceral mesoderm through concerted action of ectodermal Hedgehog and Wingless along with Hedgehog emanating from anterior termini of visceral mesodermal parasegments"J.Biol.Chem.. 278. 15571-15578 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hosono, C.: "Proteoglycan UDP-galactose : β-xylose β1,6-galactosyltrans-ferase I is essential for viability in Drosophila melanogaster"Development. 130. 41-46 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomokazu Ohshiro: "Ligand-dependent activation of breathless FGF receptor gene in Drosophila developing trachea"Mechanisms of Development. 114. 3-11 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mikiko Inaki: "Requirements of high levels of Hedgehog signaling activity for medial-region cell fate determination"Mechanisms of Development. 116. 3-18 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chie hosono: "Functional subdivision of trunk visceral mesoderm parasegments in Drosophila is required for gut and trachea development"Development. 130. 433-449 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takashi Hayashi: "Diversification of cell types in the Drosophila eye by differential expression of prepattern genes"Mechanisms of Development. 108. 13-27 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomokazu Ohshiro: "Ligand-dependent activation of breathless FGF receptor gene in Drosophila developing trachea"Mechanisms of Development. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-03-31   Modified: 2016-04-21  

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