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Quantitative and three-dimensional structural analyses of neurons and glia in various cortical regions in mice, rats and some other mammals.

Research Project

Project/Area Number 13480251
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKyushu University

Principal Investigator

KOSAKA Toshio  KYUSHU UNIVERSITY, Graduate School of Medical Science Department of Anatomy, Professor, 大学院・医学研究院, 教授 (00126054)

Co-Investigator(Kenkyū-buntansha) JINNO Shozo  KYUSHU UNIVERSITY, Graduate School of Medical Science Department of Anatomy, Assistant Professor, 大学院・医学研究院, 助手 (10325524)
FUKUDA Takaichi  KYUSHU UNIVERSITY, Graduate School of Medical Science Department of Anatomy, Associate Professor, 大学院・医学研究院, 講師 (50253414)
AIKA Yusuke  KYUSHU UNIVERSITY, Graduate School of Medical Science Department of Anatomy, Associate Professor, 大学院・医学研究院, 講師 (50021415)
小坂 克子  九州大学, 医療技術短期大学部, 助教授 (60202058)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥12,700,000 (Direct Cost: ¥12,700,000)
Keywordscerebral cortex / hippocampus / olfactory bulb / interneuron / principal neuron / astrocyte / confocal laser scanning light microscopy / electron microscopy / ペプチド / 同期 / ギャップ結合 / グリア / シナプス
Research Abstract

In this study we analyzed the structural organization of the cortical regions, that is, main olfactory bulb(MOB;paleocortex), hippocampus(archicortex) and so-called cerebral cortex(neocortex). We immunohistochemically examined the organization of the MOB In 7 mammalian species including moles, hedgehogs, tree shrews, bats and mice as well as laboratory musk shrews and rats. In this comparative study, we focused our investigation on two points : 1)whether nidi, particular spheroidal synaptic regions subjacent to glomeruli, that we previously reported in the laboratory musk shrew MOBs, are also present in other animals, and 2)whether the compartmental organization of glomeruli and two types of periglomerular cells we proposed in the rat MOBs, are general in other animals. We confirmed the presence of nidi in mole MOB and the compartmental organization of glomeruli and two types of periglomerular cells in all animals examined, which thus can be considered to be common and important organizational principles of the MOBs.
In the hippocampus we performed quantitative analyses of chemically defined GABAergic neurons and astrocytes by means of a modern stereological method, optical disector using the con focal laser scanning light microscopy. In the series of our quantitative analyses, we revealed the numerical densities and the laminar and axial differences in the distribution of GABAergic neurons containing various calcium binding proteins, neuronal nitric oxide synthase and various neuropeptides as well as astrocytes.
In addition we examined neuronal gap junctions, morphological correlates of electrical coupling, in the MOB, hippocampus and various regions of the neocortex. Our analyses on gap junctions revealed that gap junctions are rather frequent between several types of neurons ; those gap junctions appeared to interlink neuronal processes.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (62 results)

All Other

All Publications (62 results)

  • [Publications] R.Murakawa, T.Kosaka: "Structural features of mossy cells in the hamster dentate gyrus, with special reference to somatic thorny excrescences"Journal of Comparative Neurology. 429. 113-126 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kosaka, T.Kosaka: "Nidus and Tasseled cell : Distinctive neuronal organization of the main olfactory bulb of the laboratory musk shrew (Suncus murinus)."Journal of Comparative Neurology. 430. 542-561 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, N.Kinukawa, T.Kosaka: "Morphometric multivariate analysis of GABAergic neurons containing calretinin and neuronal nitiric oxide synthase in the mouse hippocampus"Brain Research. 900. 195-204 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kosaka, K.Toida, Y.Aika, T.Kosaka: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type 2 periglomerular neurons"Journal of Comparative Neurology. 440. 219-235 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Doi, H.Ishibashi, S.Jinno, T.Kosaka, N.Akaike: "Presynaptic inhibition of GABAergic miniature currents by metabotropic glutamate receptor in the rat CNS"Neuroscienc. 109. 299-311 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Patterns of expression of calcium binding proteins and neuronal nitric oxide synthase in different populations of hippocampal GABAergic neurons in mice"Journal of Comparative Neurology. 449. 1-25 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Ogata, T.Kosaka: "Structural and quantitative analysis of astrocytes in the mouse hippocampus"Neuroscience. 113. 221-233 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Immunocytochemical localization of neuronal calcium sensor-1 in the hippocampus and cerebellum of the mouse, with special reference to presynaptic terminals"Neuroscience. 113. 449-461 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Immunocytochemical characterization of hippocamposeptal projecting GABAergic nonprincipal neurons in the mouse brain : a retrograde labeling study"Brain Research. 945. 219-231 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kosaka, K.Kosaka: "Neuronal gap junctions in the rat main olfactory bulb, with special reference to intraglomerular gap junctions"Neuroscience Research. 45. 189-209 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Toida, K.Kosaka, Y.Aika, T.Kosaka: "Cathecolaminergic neurons in the olfactory bulb.(in) "Catecholamine Metabolism : From Molecular Understanding to Clinical Diagnosis and Treatment""Kluwer Academic/Plenum Publishers(T.Nagatsu, R.Nabeshima, R.McCarthy, D.Goldstein eds.). 289-292 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Compartmentation of the mouse cerebellar cortex by neuronal calcium sensor-1"Journal of Comparative Neurology. 458. 412-424 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, S.Ishizuka, T.Kosaka: "Ionic currents underlying rhythmic bursting of ventral mossy cells in the developing mouse dentate gyrus"European Journal of Neuroscience. 17. 1338-1354 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Patterns of Expression of Neuropeptides in GABAergic Nonprincipal Neurons in the Mouse Hippocampus : Quantitative Analysis with Optical Disector."Journal of Comparative Neurology. 461. 333-349 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Fukuda, T.Kosaka: "Ultrastructural study of gap junctions between dendrites of parvalbumin-containing GABAergic neurons in various neocortical areas of the adult rat"Neuroscience. 120. 5-20 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Heterogeneous expression of the cholecystokinin-like immunoreactivity in the mouse hippocampus, with special reference to the dorsoventral"Neuroscience. 122. 869-884 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kosaka, T.Kosaka: "The organization of the main olfactory bulbs of some mammals ; musk shrews, moles, hedgehogs, tree shrews, bats, mice and rats."Journal of Comparative Neurology. 472. 1-12 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Patterns of colocalization of neuronal nitric oxide synthase and somatostatin-like immunoreactivity in the mouse hippocampus : Quantitative analysis with optical disector"Neuroscience. 124. 797-808 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, T.Kosaka: "Expression and possible role of neuronal calcium sensor-1 in the cerebellum"The Cerebellum. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Murakawa, T.Kosaka: "Structural features of mossy cells in the hamster dentate gyrus, with special reference to somatic thorny excrescences"Journal of Comparative Neurology. 429. 113-126 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kosaka, T.Kosaka: "Nidus and Tasseled cell : Distinctive neuronal organization of the main olfactory bulb of the laboratory musk shrew(Suncus murinus)"Journal of Comparative Neurology. 430. 542-561 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, N.Kinukawa, T.Kosaka: "Morphometric multivariate analysis of GABAergic neurons containing calretinin and neuronal nitric oxide synthase in the mouse hippocampus"Brain Research. 900. 195-204 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kosaka, K.Toida, Y.Aika, T.Kosaka: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type 2 periglomerular neurons"Journal of Comparative Neurology. 440. 219-235 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Doi, H.Ishibashi, S.Jinno, T.Kosaka, N.Akaike: "Presynaptic inhibition of GABAergic miniature currents by metabotropic glutamate receptor in the rat CNS."Neuroscience. 109. 299-311 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Patterns of expression of calcium binding proteins and neuronal nitric oxide synthase in different populations of hippocampal GABAergic neurons in mice."Journal of Comparative Neurology. 449. 1-25 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Ogata, T.Kosaka: "Structural and quantitative analysis of astrocytes in the mouse hippocampus"Neuroscience. 113. 221-233 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Immunocytochemical localization of neuronal calcium sensor-1 in the hippocampus and cerebellum of the mouse, with special reference to presynaptic terminals"Neuroscience. 113. 449-461 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Immunocytochemical characterization of hippbcamposeptal projecting GABAergic nonprincipal neurons in the mouse brain : a retrograde labeling study"Brain Research. 945. 219-231 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kosaka, K.Kosaka: "Neuronal gap junctions in the rat main olfactory bulb, with special reference to intraglomerular gap junctions"Neuroscience Research. 45. 189-209 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Toida, K.Kosaka, Y.Aika, T.Kosaka: "Cathecolaminergic neurons in the olfactory bulb. "Catecholamine Metabolism : From Molecular Understanding to Clinical Diagnosis and Treatment""Kluwer Academic/Plenum Publishers(T.Nagatsu, R.Nabeshima, R.McCarthy, D.Goldstein eds.). 289-292 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Compartmentation of the mouse cerebellar cortex by neuronal calcium sensor-1"Journal of Comparative Neurology. 458. 412-424 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, S.Ishizuka, T.Kosaka: "Ionic currents underlying rhythmic bursting of ventral mossy cells in the developing mouse dentate gyrus"European Journal of Neuroscience. 17. 1338-1354 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Patterns of Expression of Neuropeptides in GABAergic Nonprincipal Neurons in the Mouse Hippocampus : Quantitative Analysis with Optical Disector"Journal of Comparative Neurology. 461. 333-349 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Fukuda, T.Kosaka: "Ultrastructural study of gap junctions between dendrites of parvalbumin-containing GABAergic neurons in various neocortical areas of the adult rat"Neuroscience. 120. 5-20 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Heterogeneous expression of the cholecystokinin-like immunoreactivity in the mouse hippocampus, with special reference to the dorsoventral difference"Neuroscience. 122. 869-884 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kosaka, T.Kosaka: "The organization of the main olfactory bulbs of some mammals ; musk shrews, moles, hedgehogs, tree shrews, bats, mice and rats"Journal of Comparative Neurology. 472. 1-12 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, T.Kosaka: "Patterns of colocalization of neuronal nitric oxide synthase and somatostatin-like immunoreactivity in the mouse hippocampus : Quantitative analysis with optical disector"Neuroscience. 124. 797-808 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, T.Kosaka: "Expression and possible role of neuronal calcium sensor-1 in the cerebellum"The Cerebellum. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Compartmentation of the mouse cerebellar cortex by neuronal calcium sensor-1"Journal of Comparative Neurology. 458. 412-424 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Jinno, S.Ishizuka, T.Kosaka: "Ionic currents underlying rhythmic bursting of ventral mossy cells in the developing mouse dentate gyrus."European Journal of Neuroscience. 17. 1338-1354 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Jinno, T.Kosaka: "Patterns of Expression of Neuropeptides in GABAergic Nonprincipal Neurons in the Mouse Hippocampus : Quantitative Analysis with Optical Disector"Journal of Comparative Neurology. 461. 333-349 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Fukuda, T.Kosaka: "Ultrastructural study of gap junctions between dendrites of parvalbumin-containing GABAergic neurons in various neocortical areas of the adult rat."Neuroscience. 120. 5-20 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Jinno, T.Kosaka: "Heterogeneous expression of the cholecystokinin-like immunoreactivity in the mouse hippocampus, with special reference to the dorsoventral difference."Neuroscience. 122. 869-884 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Jinno, A.Jeromin, T.Kosaka: "Expression and possible role of neuronal calcium sensor-1 in the cerebellum."The Cerebellum. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Kosaka, T.Kosaka: "The organization of the main olfactory bulbs of some mammals ; musk shrews, moles, hedgehogs, tree shrews, bats, mice and rat."Journal of Comparative Neurology. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Jinno, T.Kosaka: "Patterns of colocalization of neuronal nitric oxide synthase and somatostatin-Like immunoreactivity in the mouse hippocampus : Quantitative analysis with"Neuroscience. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.doi, H.Ishibashi, S.Jinno, T.Kosaka, N.Akaike: "Presynaptic inhibition of GABAergic miniature currents by metabotropic glutamate receptor in the rat CNS"Neuroscience. 109. 299-311 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Jinno, T.kosaka: "Patterns of expression of calcium binding proteins and neuronal nitric oxide synthase in different populations of hippocampal GABAergic neurons in mice"Journal of Comparative Neurology. 449. 1-25 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Ogata, T.Kosaka: "Structural and quantitative analysis of astrocytes in the mouse hippocampus"Neuroscience. 113. 221-233 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Immunocytochemical localization of neuronal calcium sensor-1 in the hippocampus and cerebellum of the mouse, with special reference to presynaptic terminals"Neuroscience. 113. 449-461 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Jinno, T.Kosaka: "Immunocytochemical characterization of hippocamposeptal projecting GABAergic nonprincipal neurons in the mouse brain : a retrograde labeling study"Brain Research. 945. 219-231 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kosaka, K.Kosaka: "Neuronal gap junctions in the rat main olfactory bulb, with special reference to intraglomerular gap junctions"Neuroscience Research45;189-209. 45. 189-209 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Compartmentation of the mouse cerebellar cortex by neuronal calcium sensor-1"J Comp Neurol. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Jinno, S.Ishizuaka, T.Kosaka: "Ionic currents underlying rhythmic bursting of ventral mossy cells in the developing mouse dentate gyrus"European Journal of Neuroscience. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] R.Murakawa, T.Kosaka: "Structural features of mossy cells in the hamster dentate gyrus, with special reference to somatic thorny excrescences"Journal of Comparative Neurology. 429. 113-126 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Kosaka, T.Kosaka: "Nidus and Tasseled cell : Distinctive neuronal organization of the main olfactory bulb of the laboratory musk shrew (Suncus murinus)"Journal of Comparative Neurology. 430. 542-561 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Jinno, N.Kinukawa, T.Kosaka: "Morphometric multivariate analysis of GABAergic neurons containing calretinin and neuronal nitric oxide synthase in the mouse hippocampus"Brain Research. 900. 195-204 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Kosaka, K.Toida, Y.Aika, T.Kosaka: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type periglomerular neurons"Journal of Comparative Neurology. 440. 218-235 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Doi, H.Ishibashi, S.Jinno, B.T.Kosaka, N.Akaike: "Presynaptic inhibition of GABAergic miniature currents by metabotropic glutamate receptor in the rat CNS"Neuroscience. 109. 299-311 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Ogata, T.Kosaka: "Structural and quantitative analysis of astrocytes in the mouse hippocampus"Neuroscience. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Jinno, T.Kosaka: "Patterns of expression of calcium binding proteins and neuronal nitric oxide synthase in different populations of hippocampal GABAergic neurons in mice"Journal of Comparative Neurology. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Jinno, A.Jeromin, J.Roder, T.Kosaka: "Immunocytochemical localization of neuronal calcium sensor-1 in the hippocampus and cerebellum of the mouse, with special reference to presynaptic terminals"Neuroscience. (in press).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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