| Project/Area Number |
13480280
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kanazawa University |
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Principal Investigator |
ASANO Masahide Kanazawa Univ., Advanced Science Research Center, Professor, 学際科学実験センター, 教授 (50251450)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Noriyoshi Kanazawa Univ., Advanced Science Research Center, Associate Professor, 学際科学実験センター, 助教授 (50242524)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2003: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2001: ¥6,200,000 (Direct Cost: ¥6,200,000)
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| Keywords | Carbohydrate / Knockout mice / Galactosyltransferase / Selectins / Inflammation / Skin wound healing / Embryonic lethal / 糖転移酵素遺伝子 / ガラクトース糖鎖 / 遅延型過敏症 / コンジェニックマウス |
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| Research Abstract |
Cell-to-cell, interactions are important for cell growth and differentiation. The interaction through cell surface carbohydrates is one of indispensable mechanisms among them. We have been studying on the role of carbohydrates in vivo by generating a gene knockout mouse deficient in β-1.4-galactosyhransferase-I(β4GalT-I). β4GalTs are recently found to form the gene family consisting of 7 genes, which have their own roles.
We analyzed carbohydrate structures of β4GalT-I KO mice in detail. Contribution of β4GalT-I gene to the biosynthesis of carbohydrates of various cell types was estimated by measuring Gal residues in the β1,4-linkage. Next, carbohydrate ligands of selectins, which are known to be synthesized by β4GalTs and other glycosyltransferases, were analyzed in β4GalT-I KO mice. Contribution of β4GalT-I gene to their biosynthesis was also estimated. Furthermore, Inflammatory responses of β4GalT-I KO mice and the effect of β4GalT-I deficiency were examined. In addition, the effect of β4GalT-I deficiency on skin wound healing was examined. Our results indicated that β4GalT-I plays an important role in the biosynthesis of carbohydrate ligands of selectins and their deficiency results in reduction of inflammatory responses and delayed wound healing in β4GalT-I KO mice.
While these results were obtained using β4GalT-I KO mice on mixed genetic backgrounds, we found β4GalT-I KO mice on inbred background to be lethal during late embryogenesis. Since growth retardation of the placenta rather than the embryo was remarkable several days before its death, the defect of placenta was suggested to be a cause of the embryonic lethality. Since the reason of changeable lethality depending on genetic background might be a compensatory activity by other β4GalTs, gene knockout mice deficient in another β4GalT gene were generated. Studies on elucidating the role of these p4GalT genes are in progress.
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