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Development and application of a novel capillary chromatography system utilizing vital reaction of a single cell

Research Project

Project/Area Number 13480294
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

ARAKI Tsutomu  Osaka Univ., Engineering Science, Prof., 基礎工学研究科, 教授 (50136214)

Co-Investigator(Kenkyū-buntansha) TOHNO Yoshiyuki  Nara Medical University. Prof., 医学部, 教授 (40075023)
YASUI Takeshi  Osaka Univ., Engineering Science, Res. Assist., 基礎工学研究科, 助手 (70314408)
HASHIMOTO Mamoru  Osaka Univ., Engg. Science, Assoc. Prof., 基礎工学研究科, 助教授 (70237949)
CHIKAMORI Kensuke  Naruto Univ.of Education, Assos. Prof., 学校教育学部, 助教授 (40108874)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2002: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2001: ¥9,500,000 (Direct Cost: ¥9,500,000)
KeywordsMICROSENSOR / CELL SENSOR / CAPILLARY ELECTROPHORESIS / SACCHAROMYCES CEREVISIAE / FLUORESCENCE MICROSCOPE / SECOND HARMONIC GENERATION LIGHT / PARAMECIUM CAUDATUM / FEMTOSECOND LASER / 蛍光 / 局所環境モニタリング / キャピラリー電気泳動
Research Abstract

We have proposed a biosensor chromatography system in conjunction with a capillary electrophoresis (CE) chromatography and a living cell to monitor the cellular environment. As the sensor cell for bio-CE system, Saccharomyces cerevisiae (SC) was used, SC was stained with rhodamine 123 (Rhl23) that is sensitive to electric potential of the mitochondria membrane. The cell was enveloped inside the capillary, and resultant Rh123 fluorescence image was measured with a fluorescence microscope and a CCD system.
In order to avoid unwanted electric field of 5 kV to the sensor cell, a micro-crack that works as the electric by-pass is attractive. To make the by-pass, micro-processing by a femtosecond (fs) laser was applied We have achieved to hole the glass wall of the capillary tube with a few micrometer in diameter, however the processing has not completed yet because of lack of the precision for the laser beam focusing inside the small wall. Further trial is required to succeed in the production of the micro hole. When the pulsed light from the fs laser is irradiated to the biological tissue, second harmonic generation light (SHG light) is induced. Based on this phenomenon, we have constructed a polarization SHG light detection system. We found that orientation of the collagen fiber has been informed by the SHG light.
As another trial of the cell sensor system, we have utilized a Paramecium caudatum as the sensor cell to monitor the water pollution based on the analysis of the cell moving profile in a micro-chamber. The paramecium cell showed characteristic moving profiles due to the pollutant such as heavy metal ions and acid. We have also studied the emission characteristics of a white light emitting diode to evaluate applicability to the compact light source in the present sensor system.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 島林啓太, 安井武史, 荒木 勉: "レーザエリプソメトリーを用いた細胞膜厚さのリアルタイム計測"日本機械学会第12回バイオエンジニアリング学術講演会・秋季セミナー講演論文集. 139-140 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐藤達也, 近森憲助, 荒木 勉: "細胞の活性を利用した環境モニタリングセンサに関する基礎研究"日本機械学会第14回バイオエンジニアリング講演会、講演論文集. 263-264 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 荒木 勉, 後藤 繁, 佐藤達也, 近森憲助: "ゾウリムシの遊泳と細胞蛍光を指標とした水質汚濁モニター試み"日本機械学会、関西支部第77期講演会、講演論文集. 1506 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yasui, Y.Tohno, T.Araki, et al.: "Polarimetry of second-harmonic-generation light sensitive to orientation of tissue collagen"Proc.OWLS VII. 19 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Miyata, Y.Imamoto, T.Iwata, T.Araki: "Temporal emission characteristics of white light-emitting diodes for high-speed pulsed current"Proc.SPIE (ICO-19). 695-696 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Shimabayashi, T.Yasui, Y.Tohno, T.Araki: "Imaging of the polarized Second-Harmonic-Generation light distribution to determine the orientation of collagen molecules in tissue"Proc., BOPM 2002. 268-269 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yasui, K.Nakamoto, Y.Tohno, and T.Araki: "Laser induced dynamic emission sensitive to the conformation of tissue collagen"Proc. Switzerland-Japan Workshop on New Directions in cellular and Tissue Biomechanics. 85 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Miyata, Y.Imamoto, T.Iwata, and T.Araki: "Temporal emission characteristics of white light-emitting diodes for high-speed pulsed current"Proc. SPIE (ICO-19). 695-696 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yasui, K.Shimabayashi, H.Kawaguchi, Y.Tohno, T.Iwata, and T.Araki: "Polarimetry of second-harmonic-generation lught sensitive to orientation of tissue collagen"Proc., OWLS VII, Meeting of the International Society on Optics within Life Sciences. 19 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Shimabayashi, T.Yasui, Y.Tohno, and T.Araki: "Imaging of the polarized Second-Harmonic-Generation light distribution to determine the orientation of collagen molecules in tissue"Proc. BOPM 2002. 268-269 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Sato, K.Chikamori, and T.Araki: "Study on environmental monitoring sensor using activity of a single cell"Proc. 14th Bioengineering Conf.BE-JSME(in Japanese). 263-264 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 荒木勉, 後藤繁, 佐藤達也, 近森憲助: "ゾウリムシの遊泳と細胞蛍光を指標とした水質汚濁モニター試み"日本機械学会、関西支部第77期講演会、講演論文集. 1506 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yasui, Y.Tohno, T.Araki, et al.: "Polarimetry of second-harmonic-generation light sensitive to orientation of tissue collagen"Proc. OWLS VII. 19 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Miyata, Y.Imamoto, T.Iwata, T.Araki: "Temporal emission characteristics of white light-emitting diodes for high-speed pulsed current"Proc. SPIE (ICO-19). 695-696 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Shimabayashi, T.Yasui, Y.Tohno, T.Araki: "Imaging of the polarized Second-Harmonic-Generation light distribution to determine the orientation of collagen molecules in tissue"Proc., BOPM 2002. 268-269 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yasui, K.Shimabayashi, Y.Tohno.T.Araki: "Polarimetric imaging of SHG light for spatial distribution measurement of collagen orientation in biological tissue"Proc., CLEO/Pacific RIM 2003. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yasui, K.Nakamoto, Y.Tohno, T.Araki: "Laser induced dynamic emission sensitive to the conformation of tissue collagen"Switzerland-Japan Workshop (Les Diablerets, Switzerland Sep. 24-28. 2001). 85 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤達也, 近森憲助, 荒木勉: "細胞の活性を利用した環境モニタリングセンサに関する基礎研究"第14回バイオエンジニアリング講演会、講演論文集. No.02-04. 263-264 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 荒木勉, 後藤繁, 佐藤達也, 近森憲助: "ゾウリムシの遊泳と細胞蛍光を指標とした水質汚濁モニター試み"日本機械学会、関西支部第77期講演会、講演論文集. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ysui, Y.Tohno, T.Araki, et al.: "Polarimetry of second-harmonic-generation light sensitive to orientation of tissue collagen"Proc. OWLS VII. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Miyata, Y.Imamoto, T.Iwata, T Araki: "Temporal emission characteristics of white light-emitting diodes for high-speed pulsed current"Proc. SPIE(ICO-19). (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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