Multistage dielectrophoretic separation of various kinds of biological microparticles with quadrupole microelectrode capillary
| Project/Area Number |
13554032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Osaka University |
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Principal Investigator |
TSUKAHARA Satoshi Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (50207338)
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| Co-Investigator(Kenkyū-buntansha) |
WATARAI Hitoshi Graduate School of Science, Professor, 大学院・理学研究科, 教授 (30091771)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥12,700,000 (Direct Cost: ¥12,700,000)
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| Keywords | Dielectrophoresis / Fluorescence Microscope / Field flow fractionation / Planar quadrupole electrode / Biological microparticles / Lectin protein / Concanavalin A / Yeast cell / デキストラン |
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| Research Abstract |
Dielectrophoresis of single microparticles (0.35 - 7.3 μm in radius) and DNA (40 kbp) were measured in a planar quadrupole microelectrode (50 μm or 65 μm in working area radius) with a fluorescence microscope. An alternating current (ac) of 1 kHz - 10 MHz in frequency and 0 - 5 V in voltage was applied to the quadrupole microelectrode. Polystyrene, polystyrene-carboxylate and carbon microparticles, and yeast cell were adopted as a sample microparticle. The dielectrophoretic mobilities of the single microparticles and single DNA were analyzed quantitatively with their intrinsic and surface conductivities, their permittivity, and their size as well as the conductivity and permittivity of aqueous media. Some separations among the microparticles were demonstrated with a fabricated capillary quadrupole microelectrode (82.5 μm in radius of working area, 2.35 cm in length) by combining the technique of field flow fractionation.
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Report
(3 results)
Research Products
(11 results)
