|Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥6,000,000 (Direct Cost: ¥6,000,000)
(1) Submerged culture of useful mushroom mycelium: We found new type of Tricoloma matsutake mycelium of which growth rate was five time as high as that of original species in our laboratory. This new type of mycelium was cultured in a standard bubble column to find the optimum conditions on medium composition, pH, and aeration rate (Kawagoe and Itoh ; Research Reports of Nara National College of Technology, 38, 83-86 (2002)). Also, we estimated apparent viscosity of suspended medium of T. matsutake mycelium from the circulation velocity in an external-loop airlift column (Kawagoe ; The Japanese society for multiphase flow, Annual Meeting 2002, B118 (2002)). Optimum conditions on aeration rate, pH, and medium compositions were also found for submerged culture of Agaricus blazei in a standard bubble column bioreactor (Kawagoe. ; unpublished).
(2) Measurement of mycelium pellets concentration by laser transmission method : We developed a novel measurement method of mycelium pellets concent
ration by laser transmission. From the laser transmission and pulse width, concentration and size of mycelium pellets were estimated simultaneously. We investigated the effects of pellet size distribution and diameter of laser, flux. As a result, this method was found to be able to apply in practical purposes (Kihara, Kawagoe, and Noda ; Chemical Engineering Symposium Series 77, 52-57 (2003)).
(3) Development of separation methods of useful substances by reverse micelles: We investigated protein extraction using AOT reverse micelles and found a new method in which extraction efficiency was extremely increased. This method was found to be effective in recovering extracted protein (backward extraction) from the AOT reverse micellar organic phase (Naoe et al ; Biochem. Eng. J., 10, 137-142 (2002)). Employing reverse micelles of sugar esters, we succeeded in extracting proteins with large molecular weight which was difficult to extract in previous method (Naoe et al; Japan Society for Food Engineering, Third Annual Meeting, PF-7 (2002)). Enzyme stability was evaluated by using Lipase-catalyzed hydrolysis (15th International Symposium on Plant Lipids, p.7-8 (2002), Naoe et al; International Congress on Biocatalysis 2002, P056 (2002)). We also investigated lipase-catalyzed esterification in microemulsion-based organogels with gelatin (Nagayama et al; International Congress on Biocatalysis 2002, P038 (2002)). Less