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Study on inhibitors of vacuolar-type H^+-pyrophosphatase to search specific medicines for malaria disease

Research Project

Project/Area Number 13556013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionNagoya University

Principal Investigator

MAESHIMA Masayoshi  Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (80181577)

Co-Investigator(Kenkyū-buntansha) OJIKA Makoto  Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (50152492)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥6,600,000 (Direct Cost: ¥6,600,000)
Keywordsvacuolar membrane / H^+-pyrophosphatase / inhibitor / malaria / proton pump / acylspermidine / soft coral / マラリア原虫 / 海洋生物 / H^+ピロホスファターゼ / マラリア病原虫
Research Abstract

The most virulent form of malaria is caused by the protozoan, Plasmodiumfalciparum, which is transmitted to humans by the bite of Anopheles mosquito. Key challenges to combating this devastating disease are the spread of resistance to antimalarial drugs. We pay attention to a fact that H^+-pyrophosphatase (H^+-PPase) exists in these protozoa but not in animal cells. Thus, inhibitors of H^+-PPase are expected as a good candidate of antimalarial drug. During this study, we prepared water-soluble and organic-solvent soluble fractions from 145 marine organisms, and examined their effects on H^+-PPase. We found that a few compounds in the water-soluble fraction prepared from a soft coral inhibited H^+-PPase. These are acylspermidine derivatives, which are N',N",N"-trimethylspermidines athat are acylated by a methylbranched unsaturated fatty acid. Acylspermidines inhibited PPi hydrolysis activity and PPi-dependent H^+ translocation (half inhibition concentrations, 1 μM). These compounds actually inhibited H^+-PPase and prevent acidification of vacuoles in living protoplasts.
We also obtained the following new information on H^+-PPase.
(1) Mutant Arabidopsis plants that lack the H^+-PPase gene were extremely small. This observation indicates that H^+-PPase is essential for normal growth of plants.
(2) Two essential domains, which are involved in PPi hydrolysis, were determined by site-directed mutagenesis of mung bean H^+-PPase.
(3) Molecular properties of the PPi-dependent H^+ current through H^+-PPase were directly determined by patch clamp analysis.
(4) We established experimental conditions for expressing Streptomyces H^+-PPase in E.coli cells with a high efficiency to investigate the structure-function relationship of the enzyme.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (107 results)

All Other

All Publications (107 results)

  • [Publications] Frangne, N.: "Expression and distribution of vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes."Planta. 212. 270-278 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs."Planta. 212. 294-304 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakanishi, Y: "Mutagenic analysis of functional residues in putative substrate binding site and acidic domains of vacuolar H^+-pyrophosphatase."Journal of Biological Chemistry. 276. 7654-7660 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeshima, M.: "Tonoplast transporters : organization and function."Annual Review of Plant Physiology and Plant Molecular Biology. 52. 469-497 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamura, Y.: "ATP analogue binding to the A subunit induces conformational change of the E subunit that involves a disulfide bond formation in plant V-ATPase."European Journal of Biochemistry. 268. 2801-2809 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuasa, K.: "Organ specificity of a vacuolar C^<2+>-binding protein RVCaB in radish and its expression under Ca^<2+>-deficient conditions"Plant Molecular Biology. 47. 633-640 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima, Y.: "Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense : comparison with radish."Plant and Cell Physiology. 42. 1119-1129 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jiang, L.: "The protein storage vacuole : a unique compound organelle."Journal of Cell Biology. 155. 991-1002 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, M.: "Expression of V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and its complementation study."European Journal of Biochemistry. 268. 6097-6104 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, M.: "Functional expression of Acetabularia acetabulum vacuolar H^+-pyrophosphatase in a yeast VMA3-deficient strain."Journal of Exerimental Botany. 53. 2273-2275 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuasa, K.: "Equilibrium dialysis measurements of the Ca^<2+>-properties of recombinant radish vacuolar Ca^<2+>-binding protein expressed in E.coil."Biosciences Biotechnology and Biochemistry. 66. 2382-2387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Aauaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings."Plant and Cell Physiology. 43. 1229-1237 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiwara, S.: "Molecular properties of a unique matrix attachment region-binding protein in tobacco cells."Plant and Cell Physiology. 43. 1558-1567 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yang, S.: "Modulation of vacuolar H^+-pumps and aquaporin by phytohormones in rice seedling leaf sheaths."Biol.Pharm.Bull.. 26. 88-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mimura, T.: "Rapid increase of vacuolar volume in response to salt stress."Planta. 216. 397-402 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "An abundance of aquaporins in specific cells and osmotic water permeability of individual protoplasts of radish."Plant and Cell Physiology. 44. 277-286 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirono, M.: "Acylspermidine derivatives isolated from a soft coral Sinularia sp.inhibits plant vacuolar H^+-pyrophosphatase"Journal of Biochemistry. 133. 811-816 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakanishi, Y.: "Patch clamp analysis of H^+ pump expressed in giant yeast vacuoles"Journal of Biochemistry. 134. 615-623 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamiya, T.: "Residues in internal repeats of the rice cation/H^+ exchanger are involved in the transport and selection of cations."Journal of Biological Chemistry. 279. 812-819 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda, A.: "Effect of salt and osmotic stresses on the expression of genes for the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley."Journal of Experimetal Botany. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Geisler, M: "Arabidopsis immunophilin-kike TWD1 modulates vacuolar ABC transporter activity by means of protein-protein interaction."Molecular Biology of the Cell. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中西 洋一: "液胞のCa^<2+>集積とプロトンポンプ"蛋白質核酸酵素. 47. 1777-1782 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 須賀 しのぶ: "液胞膜の分化と多様性"細胞工学別冊 植物細胞工学. 17. 190-195 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島 正義: "液胞膜H^+-ピロホスファターゼと膜ダイナミクス"細胞工学. 12. 1522-1527 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中西 洋一: "液胞型H^+ポンプ"細胞工学別冊 植物細胞工学. 18. 4-39 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三村 久敏: "膜輸送システムの分子構造と機能"細胞工学別冊 植物細胞工学. 18. 199-205 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島 正義: "膜輸送系の研究に利用される阻害剤と蛍光プローブ"細胞工学別冊 植物細胞工学. 18. 224-229 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島 正義: "液胞と代謝-物質集積とコミュニケーション"蛋白質・核酸・酸素. 48. 2184-2191 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島正義: "ストレスの植物生化学・分子生物学 (第14章、第15章分担執筆)"学会出版センター. 308 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島正義: "朝倉植物生理学講座 第2巻代謝(第3章エネルギー代謝分担執筆)"朝倉書店. 175 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeshima, M.: "Handbook of Plant Growth.pH as a Master Variable in Plant Growth (分担執筆)"Marcel Dekker Inc.,. 446 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Frangne, N., Maeshima, M., Schaffner, A.R., Mandel, T., Martinoia, E., Bonnemain, J.L.: "Expression and distribution of vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes."Planta. 212. 270-278 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S., Imagawa, S., Maeshima, M.: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs."Planta. 212. 294-304 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hara-Nishimura, I., Maeshima, M.: "Vacuolar processing enzymes and aquaporins."Annual Plant Reviews, CRC Press. 5. 20-42 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakanishi, Y., Saijo, T., Wada, Y., Maeshima, M.: "Mutagenic analysis of functional residues in putative substrate binding site and acidic regions of vacuolar H^+-pyrophosphatase."J.Biol.Chem.. 276. 7654-7660 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeshima, M.: "Tonoplast transporters : organization and function."Annu.Rev.Plant Physiol.Plant Mol.Bid.. 52. 469-497 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamura, Y., Arakawa, K., Maeshima, M., Yoshida, S.: "ATP analogue binding to the A subunit induces conformational change of the E subunit that involves a disulfide bond formation in plant V-ATPase."Eur J.Biochem.. 268. 2801-2809 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuasa, K., Maeshima, M.: "Organ specificity of a vacuolar Ca^<2+>-binding protein RVCaB in radish and its expression under Ca^<2+>-deficient conditions."Plant Mol.Biol.. 47. 633-640 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima, Y., Iwasaki, I., Murakami, M., Suga, S., Maeshima, M.: "Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense : comparison with radish."Plant Cell Physiol.. 42. 1119-1129 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jiang, L.Phillips, T.E., Hamm, C.A., Drozdowicz, YM., Rea, P.A., Maeshima, M., Rogers, S.W., Rogers, J.C.: "The protein storage vacuole : a unique compound organelle."J.Cell Biol.. 155. 991-1002 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, M., Hinohara, M., Umami, K., Okada, Y., Wada, Y, Nakanishi, Y., Maeshima, M.: "Expression of V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saceharomyces cerevisiae and its complementation study."Eur.J.Biochem.. 268. 6097-6104 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, M., Umami, K., Hinohara, M., Tanimura, Y., Ohmae, A., Nakanishi, Y, Maeshima, M.: "Functional expression of Acetabularia acetabulum vacuolar H^+-pyrophosphatase in a yeast VMA3-deficient strain."J.Exp.Botany. 53. 2273-2275 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuasa, K., Maeshima, M.: "Equilibrium dialysis measurements of the Ca^<2+>-binding properties of recombinant radish vacuolar Ca^<2+>-binding protein expressed in E.coli."Biosci.Biotechnol.Biochem.. 66. 2382-2387 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S., Komatsu, S., Maeshima, M.: "Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings."Plant Cell Physiol.. 43. 1229-1237 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiwara, S., Matsuda, N., Sato, T., Sonobe, S., Maeshima, M.: "Molecular properties of a unique matrix attachment region-binding protein in tobacco cells."Plant Cell Physiol.. 43. 1558-1567 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mimura T., Kura-Hotta M., Tsujimura T., Ohnishi M., Miura M., Okazaki Y., Mimura M., Maeshima M., Washitani-Nemoto S.: "Rapid increase of vacuolar volume in response to salt stress."Planta. 216. 397-402 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yang, S., Maeshima, M., Tanaka, Y., Koniatsu, S.: "Modulation of vacuolar He-pumps and aquaporin by phytohormones in rice seedling leaf sheaths."Biol.Pharm.Bull.. 26. 88-92 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S., Murai, M., Kuwagata, T., Maeshima, M.: "An abundance of aquaporins in specific cells and osmotic water permeability of individual protoplasts of radish."Plant Cell Physiol.. 44. 277-286 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirono, M., Ojika, M., Mimura, H., Nakanishi, Y., Maeshima, M.: "Acylspermidine derivatives isolated from a soft coral Sinularia sp.inhibits plant vacuolar H^+-pyrophosphatase."J.Biochem.. 133. 811-816 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakanishi, Y, Yabe, I., Maeshima, M.: "Patch clamp analysis of H^+ pump expressed in giant yeast vacuoles."J.Biochem.. 134. 615-623 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamiya, T., Maeshima, M.: "Residues in internal repeats of the rice cation/H^+ exchanger are involved in the transport and selection of cations."J.Biol.Chem.. 279. 812-819 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda, A., Chiba, K., Maeda, M., Nakamura, A., Maeshima, M., Tanaka, Y.: "Effect of salt and osmotic stresses on the expression of genes for the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley."J.Exp.Botany.. 55. 585-594 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Geisler, M., Girin, M., Brnadt, S., Vincenzetti, V., Plaza, S., Paris, N., Kobae, Y, Maeshima, M., Billion, K., Kolukisaoglu, U.H., Schulz, B., Martinoia, B.: "Arabidopsis immunophilin-kike TWD1 modulates vacuolar ABC transporter activity by means of protein-protein interaction."Mol.Biol.Cell. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Frangne, N.: "Expression and distribution of vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes"Planta. 212. 270-278 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suga, S.: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs"Planta. 212. 294-304 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakanishi, Y.: "Mutagenic analysis of functional residues in putative substrate binding site and acidic domains of vacuolar H^+-pyrophosphatase"Journal of Biological Chemistry. 276. 7654-7660 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Maeshima, M.: "Tonoplast transporters : organization and function"Annual Review of Plant Physiology and Plant Molecular Biology. 52. 469-497 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawamura, Y.: "ATP analogue binding to the A subunit induces conformational change of the E subunit that involves a disulfide bond formation in plant V-ATPase"European Journal of Biochemistry. 268. 2801-2809 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuasa, K.: "Organ specificity of a vacuolar Ca^<2+>-binding protein RVCaB in radish and its expression under Ca^<2+>-deficient conditions"Plant Molecular Biology. 47. 633-640 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohshima, Y.: "Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense : comparison with radish"Plant and Cell Physiology. 42. 1119-1129 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jiang, L.: "The protein storage vacuole : a unique compound organelle"Journal of Cell Biology. 155. 991-1002 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikeda, M.: "Expression of V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and its complementation study"European Journal of Biochemistry. 268. 6097-6104 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikeda, M.: "Functional expression of Acetabularia acetabulum vacuolar H^+-pyrophosphatase in a yeast VMA3-deficient strain"Journal of Experimental Botany. 53. 2273-2275 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuasa, K.: "Equilibrium dialysis measurements of the Ca^<2+>-binding properties of recombinant radish vacuolar Ca^<2+>-binding protein expressed in E.coli"Biosciences Biotechnology and Biochemistry. 66. 2382-2387 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suga, S.: "Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings"Plant and Cell Physiology. 43. 1229-1237 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujiwara, S.: "Molecular properties of unique matrix attachment region-binding protein in tobacco cells"Plant and Cell Physiology. 43. 1558-1567 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yang, S.: "Modulation of vacuolar H^+-pumps and aquaporin by photohormones in rice seedling leaf sheaths"Bio.Pharm.Bull.. 26. 88-92 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mimura, T.: "Rapid increase of vacuolar volume in response to salt stress"Planta. 216. 397-402 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suga, S.: "An abundance of aquaporins in specific cells and osmotic water permeability of individual protoplasts of radish"Plant and Cell Physiology. 44. 277-286 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirono, M.: "Acylspermidine derivatives isolated from a soft coral Sinularia sp. inhibits plant vacuolar H^+-pyrophosphatase"Journal of Biochemistry. 133. 811-816 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakanishi, Y.: "Patch clamp analysis of H^+ pump expressed in giant yeast vacuoles"Journal of Biochemistry. 134. 615-623 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamiya, T.: "Residues in internal repeats of the rice cation/H^+ exchanger are involved in the transport and selection of cations"Journal of Biological Chemistry. 279. 812-819 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fukuda, A.: "Effect of salt and osmotic stresses on the expression of genes for the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley"Journal of Experimetal Botany. 55. 585-594 (2004)

    • Related Report
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  • [Publications] Geisler, M: "Arabidopsis immunophilin-kike TWD1 modulates vacuolar ABC transporter activity by means of protein-protein interaction"Molecular Biology of the Cell. (In press). (2004)

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  • [Publications] 中西 洋一: "液胞のCa^<2+>集積とプロトンポンプ"蛋白質核酸酵素. 47. 1777-1782 (2002)

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  • [Publications] 須賀 しのぶ: "液胞膜の分化と多様性"細胞工学別冊 植物細胞工学. 17. 190-195 (2002)

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  • [Publications] 前島 正義: "液胞膜H^+-ピロホスファターゼと膜ダイナミクス"細胞工学. 12. 1522-1527 (2002)

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  • [Publications] 中西 洋一: "液胞型H^+-ポンプ"細胞工学別冊 植物細胞工学. 18. 4-39 (2003)

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  • [Publications] 三村 久敏: "膜輸送システムの分子構造と機能"細胞工学別冊 植物細胞工学. 18. 199-205 (2003)

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  • [Publications] 前島 正義: "膜輸送系の研究に利用される阻害剤と蛍光プローブ"細胞工学別冊 植物細胞工学. 18. 224-229 (2003)

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  • [Publications] 前島 正義: "液胞と代謝-物質集積とコミュニケーション"蛋白質・核酸・酵素. 48. 2184-2191 (2003)

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  • [Publications] 前島正義: "ストレスの植物生化学・分子生物学(第14章、第15章分担執筆)"学会出版センター. 308 (2001)

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  • [Publications] 前島正義: "朝倉植物生理学講座 第2巻代謝(第3章エネルギー代謝分担執筆)"朝倉書店. 175 (2001)

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  • [Publications] Maeshima, M.: "Handbook of Plant Growth. pH as a Master Variable in Plant Growth(分担執筆)"Marcel Dekker, Inc.. 446 (2002)

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  • [Publications] Ikeda, M.: "Functional expression of Acetabularia acetabulum vacuolar H^+-pyrophosphatase in a yeast VMA3-deficient strain"J. Experimental Botany. 53. 2273-2275 (2002)

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  • [Publications] Yuasa, K.: "Equilibrium dialysis measurements of the Ca^<2+> -binding properties of recombinant radish vacuolar Ca^<2+> -binding protein expressed in E. coli"Biosc. Biotechnol. Biochem.. 66. 2382-2387 (2002)

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  • [Publications] Suga, S.: "Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings"Plant Cell Physiol.. 43. 1229-1237 (2002)

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  • [Publications] Pittman, J.K.: "Distinct N-terminal regulatory domains of Ca^<2+/> H^+ antiporters"Plant Physiology. 130. 1054-1062 (2002)

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  • [Publications] Fujiwara, S.: "Molecular properties of a unique matrix attachment region-binding protein in tobacco cells"Plant Cell Physiol.. 43. 1558-1567 (2002)

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  • [Publications] Yang, S.: "Modulation of vacuolar H^+ -pumps and aquaporin by phytohormones in rice seedling leaf sheaths"Biol. Pharm. Bull.. 26. 88-92 (2002)

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  • [Publications] Mimura, T.: "Rapid increase of vacuolar volume in response to salt stress"Planta. 216. 397-402 (2003)

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  • [Publications] Hagiwara, Y.: "Subcellular localization of host and viral proteins associated with tobamovirus RNA replication"EMBO J.. (In press). (2003)

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  • [Publications] Suga, S.: "An abundance of aquaporins in specific cells and osmotic water permeability of individual protoplasts of radish"Plant Cell Physiol.. 44(In press). (2003)

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  • [Publications] 中西 洋一: "液胞のCa^<2+>集積とプロトンポンプ"蛋白質核酸酵素. 47. 1777-1782 (2002)

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      2002 Annual Research Report
  • [Publications] 須賀 しのぶ: "液胞膜の分化と多様性"細胞工学別冊 植物細胞工学. 17. 190-195 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 前島 正義: "液胞H^+-ピロホスファターゼと膜ダイナミクス"細胞工学. 12. 1522-1527 (2002)

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  • [Publications] 中西 洋一: "液胞型H^+ポンプ"細胞工学別冊 植物細胞工学. 18(印刷中). (2003)

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  • [Publications] 三村 久敏: "膜輸送システムの分子構造と機能"細胞工学別冊 植物細胞工学. 18(印刷中). (2003)

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  • [Publications] 前島 正義: "膜輸送系の阻害剤"細胞工学別冊 植物細胞工学. 18(印刷中). (2003)

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  • [Publications] Maeshima, M.: "Handbook of Plant Growth. pH as a Master Variable in Plant Growth"Marcel Dekker, Inc.. 446 (2002)

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  • [Publications] 広野めぐみ: "第24回生体膜と薬物の相互作用シンポジウム(ISSN : 0919-2131)"日本薬学会. 330 (2002)

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  • [Publications] Nakanishi, Y.: "Mutagenic analysis of functional residues in putative substrate binding site and acidic domains of vacuolar H^+-pyrophosphatase"Journal of Biological Chemistry. 276. 7654-7660 (2001)

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  • [Publications] Maeshima, M.: "Tonoplast transporters : organization and function"Annual Review of Plant Physiology and Plant Molecular Biology. 52. 469-497 (2001)

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  • [Publications] Kawamura, Y.: "ATP analogue binding to the A subunit induces conformational change of the E subunit that involves a disulfide bond formation in plant V-ATPase"European Journal of Biochemistry. 268. 2801-2809 (2001)

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  • [Publications] Yuasa, K.: "Organ specificity of a vacuolar Ca^<2+>-binding protein RVCaB in radish and its expression under Ca^<2+>-deficient conditions"Plant Molecular Biology. 47. 633-640 (2001)

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  • [Publications] Ikeda, M.: "Expression of V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and its complementation study"European Journal of Biochemistry. 268. 6097-6104 (2001)

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  • [Publications] Maeshima, M.: "Handbook of Plant Growth.pH as a Master Variable in Plant Growth"Marcell Dekker,Inc.(New York). 23-47 (2002)

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Published: 2001-04-01   Modified: 2016-04-21  

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