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Whole-genome sequencing of a psychrophilic bacterium, analysis of genes involved in cold adaptation, and exploitation of cold-active enzymes

Research Project

Project/Area Number 13556014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ESAKI Nobuyoshi  Inst. Chem. Res., Kyoto Univ., Professor, 化学研究所, 教授 (50135597)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Hiroshige  Takara Bio Inc., Project Manager, プロジェクトマネージャー
MIHARA Hisaaki  Inst. Chem. Res., Kyoto Univ., Instructor, 化学研究所, 助手 (30324693)
KURIHARA Tatsuo  Inst. Chem. Res., Kyoto Univ., Instructor, 化学研究所, 助手 (70243087)
山田 英  ドラゴンジェノミクス(株), 取締役副社長
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2002: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2001: ¥9,300,000 (Direct Cost: ¥9,300,000)
Keywordspsychrophilic bacteria / psychrophiles / psychrotrophs / Shewanella / Acinetobacter / genome analysis / cold adaptation / cold-active enzymes / 好冷酵素 / 形質転換 / 宿主-ベクター系 / Shewanella sp.Ac10 / Acinetobacter sp.strain no.6 / 宿主ベクター系 / 脂質分解酵素
Research Abstract

1. We carried out whole-genome analysis of Shewanella sp. Ac10, a psychrophile isolated from the Antarctic Ocean. The genome size was 4.5 Mbp, and the number of ORFs was about 4, 800.
2. Shewanella sp. Ac10 grown at 4℃ produced a large quantity of a protein homologous to a cold-shock protein, CspA, from Escherichia coli.
3. A collagenase of Shewanella sp. Ac1O was overproduced and characterized.
4. We established a transformation method for Shewanella sp. Ac1O, isolated a promoter, and constructed an expression vector.
5. A psychrotrophic bacterium, Acinetobacter sp. strain no. 6, was isolated from Siberian tundra soil. The bacterium produced extracellular lipolytic enzymes, and degraded various triglycerides in the medium at low temperatures. The bacterium is expected to be useful for the waste water treatment at low temperatures.
6. The gene coding for a cold-active lipolytic enzyme with a substrate preference for fatty acid vinyl esters was cloned from Acinetobacter sp. strain no. 6. The enzyme was shown to be useful for the synthesis of esters by transesterification using vinyl esters as an acyl donor.
7. We cloned the gene coding for a cold-active esterase from Acinetobacter sp. strain no. 6 that belongs to the EST group of the esterase/lipase family.
8. We constructed a vector to introduce exogenous genes into Acinetobacter sp. strain no. 6 and isolated powerful promoters suitable for overexpression of exogenous genes.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Suzuki, Takeshi et al.: "Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain No."J. Biosci. Bioeng.. 92(2). 144-148 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki, Takeshi et al.: "A cold-active esterase with a substrate preference for vinyl esters from a psychrotroph, Acinetobacter sp. strain no.6"J. Mol. Catal. B-Enzym.. 16. 255-263 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki, Takeshi et al.: "Primary structure and catalytic properties of a cold-active esterase from a psychrotroph, Acinetobacter sp. strain no.6 isolated from Siberian soil"Biosci. Biotechnol. Biochem.. 66. 1682-1690 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wei, Yun-lin et al.: "A novel esterase from a psychrotrophic bacterium, Acinetobacter sp. strain no.6,that belongs to the amidase signature family"J. Mol. Catal. B-Enzym.. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki, Takeshi et al.: "Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain No. 6"J. Biosci. Bioeng.. 92(2). 144-148 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki Takeshi et al.: "A cold-active esterase with a substrate preference for vinyl esters from a psychrotroph, Acinetobacter sp. strain no. 6"J. Mol. Catal. Enzym.. 16. 255-263 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki Takeshi et al.: "Primary structure and catalytic properties of a cold-active esterase from a psychrotroph, Acinetobacter sp. strain no. 6 isolated from Siberian soil"Biosci. Biotechnol. Biochem.. 66. 1682-1690 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wei, Yun-lin et al.: "A novel esterase from a psychrotrophic bacterium, Acinetobacter sp. strain no. 6, that belongs to the amidase signature family"J. Mol. Catal. Enzym.. in press. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suzuki, Takeshi et al.: "A cold-active esterase with a substrate preference for vinyl esters from a psychrotroph, Acinetobacter sp.strain no.6: gene cloning, purification, and characterization"J.Mol.Catal.B : Enzym.. 16. 255-263 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, Takeshi et al.: "Primary structure and catalytic properties of a cold-active esterase from a psychrotroph, Acinetobacter sp.strain no.6, isolated from Siberian soil"Biosci.Biotechnol.Biochem.. 66・8. 1682-1690 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Wei, Yun-lin et al.: "A novel esterase from a psychrotrophic bacterium, Acinetobacter sp.strain no.6, that belongs to the amidase signature family"J.Mol.Catal.B : Enzym.. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, Takeshi et al.: "Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain No.6"J.Biosci.Bioeng.. 92(2). 144-148 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, Takeshi et al.: "A cold-active esterase with a substrate preference for vinyl ester from a psychrotroph, Acinetobacter sp. strain no.6"J.Mol.Catal.B-Enzym.. 16. 255-263 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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