| Project/Area Number |
13556024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
林産学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIMADA Mikio KYOTO UNIVERSITY, WOOD RESEARCH INSTITUTE, PROFESSOR, 木質科学研究所, 教授 (50027166)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Tomonori JAPAN HYGIENE CENTER, OSAKA BRANCH, DIRECTOR, 大阪支社・中央研究所, 所長
HATTORITA Takefumi KYOTO UNIVERSITY, WOOD RES.INSTITUTE, INSTRUCTOR, 木質科学研究所, 助手 (60212148)
TSUNODA Kunio KYOTO UNIVERSITY, WOOD RES.INSTITUTE, ASS.PROFESSOR, 木質科学研究所, 助教授 (30127104)
ASAI Taakehito SANKYOU CHEMICALS CO.LTD, CHEMICAL RESEARCH INSTITUTE, SENIOR RESEARCHER, 上級研究員
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | Fomitopsis palustris / copper-tolerant fungi / isocitrate lyase / oxalic acid / wood-rotting fungi / malate suynthase / enzyme injibitors / glyoxylate cycle / 木材防腐菌 / 木材防腐剤 |
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| Research Abstract |
The primary objective of this research project is to develop a new screening system for wood preservatives on the basis of biochemical analysis of the inhibitors blocking the key enzymes of the copper-tolerant wood rotting fungi. First we investigated metabolic mechanism for biosynthesis of oxalic acid which causes detoxification of copper preservatives, and finally, attempted to test many compounds if they inhibit potently the key enzyme isocitrate lyase in the in vitro inhibitor screening system.
The following important findings obtained have been published in the international journals listed at the end of this abstract.
1) The brown-rot fungus Fomitopsis palustris contains constitutively the glyoxylate cycle in contrast with the normal microorganisms grown on glucose and the cycle linked with the TCA cycle was also found to couple with the oxalic acid biosynthesis.
2) Through these metabolic cycles coupling with the oxalate biosynthesis, the wood-rotting fungi seem to gain the energy for their growth and one of the key enzymes was found to be isocitrate lyase (ICL) which plays a pivotal role for biosynthesis of oxalic acid.
3) Both ICL and malate synthase (MS) which are key enzymes of the glyoxylate cycle were found to distribute widely among the both white-rot and brown-rot fungi.
4) We have successfully purified these key enzymes (ICL and MS) for the first time from the wood-rotting fungi and characterized for comparison with the enzymes from other sources.
5) We also succeeded in making fruit bodies of the F.palustris in the liquid culture for the first time.
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