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Use of interferon-τ for generation of viral resistant animals.

Research Project

Project/Area Number 13556050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied veterinary science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

KAZUHIKO Imakawa  THE UNIVERSITY OF TOKYO, The University of Tokyo Graduate school of Agricaltural and Life Sciences, Associate Professor (00291956)

Co-Investigator(Kenkyū-buntansha) SAKAI Senkiti  東京大学, Graduate school of Agricaltural and Life Sciences, Professor (80114487)
TOHYA Yukinobu  東京大学, Graduate school of Agricaltural and Life Sciences, Associate Professor (20180119)
MIYAZAWA Takayuki  Osaka University, Inst.of Viral Research, Research Associate (80282705)
SENTUI Hiroshi  独立行政法人・農業技術研究機構, National Institute of Animal Health, Research Leader (Researcher)
泉対 博  独立行政法人農業技術研究機構, 動物衛生研究所, 室長(研究職)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2002: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2001: ¥7,300,000 (Direct Cost: ¥7,300,000)
Keywordsinterferon-τ / Live Vector / Transposon / Enhancer / Promoter / Foamy Virus / HIV / Virai resistance / コアクチベーター / 産業動物 / 抗ウイルス活性 / 遺伝子発現制御
Research Abstract

For the continuous and low expression of interferon-τ(IFNτ), numerous experiments were conducted to determine the upstream region of this gene that allow such expression. At the beginning, the studies were conducted with the use of human choriocarcinoma cells, which provided the importance of AP-1 site located at the enhancer region of the IFNτ gene. However, the subsequent experiments with HTS-1 cells, established from goat placenta, provided that Ets-2 site at the promoter region was also important in IFNτ gene transcription. Later, we have identified the importance of coactivator CBP for the transcription, but we realized that the use of the upstream region par se may not allow the low and continuous expression of this gene.
For these reasons, we examined a new strategy that requires the use of so-called "live vector" for the integration and expression of IFNτ gene. The open reading frame region of IFNτ gene was inserted to human foamy virus genome (FV), which was used for transient and stable transfection studies. Cells transduced with this construct exhibited resistance to HIV infection. Further, effectiveness of transposon genome was also tested for effective integration and expression of a foreign gene. This, too, was very promising to satisfy the original objective. However, both FV and transposon require further analyses for the continuous and low expression of IFNτ gene, possibly resulting in the production of animals resistant to viral infection.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All 2003 2002 2001 Other

All Journal Article (13 results) Book (1 results) Patent(Industrial Property Rights) (1 results) Publications (12 results)

  • [Journal Article] Regulation of blastocyst migration. apposition, and initial adhesion by a chemok-Ine, interferon γ inducible protein 10kDa(IP-10) during early gestation2003

    • Author(s)
      Nagaoka, その他
    • Journal Title

      Journal of Biological Chemistry (In Press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A Potential Live Vector, Foamy Virus, Directed Intra-Cellular Expression of Ovine Interferon-τ Exhibited the Resistance to HIV Infection2003

    • Author(s)
      Fujii, その他
    • Journal Title

      Journal of Veterinary Medical Science (In Press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Involvement of coactivator CBP in the regulation of conceptus interferon-τ gene eapression2003

    • Author(s)
      Xu, その他
    • Journal Title

      Molecular Reproduction and Development 65

      Pages: 23-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A chemokine, interferon (IFN)-γ-inducible protein 10 kDa,is stimulated by IFN-τ and recruits immune cells in the ovine endometrium2003

    • Author(s)
      Nagaoka, その他
    • Journal Title

      Biology of Reproduction 68

      Pages: 1413-1421

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Regulation of blastocyst migration, apposition, and initial adhesion by a chemokine, interferon γ inducible protein 10 kDa during early gestation.2003

    • Author(s)
      Nagaoka K, others.
    • Journal Title

      Journal of Biological Chemistry (In Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A potential live vector, foamy virus, directed intra?cellular expression of ovine interferon-T exhibited the resistance to HIV infection.2003

    • Author(s)
      Fujii, others.
    • Journal Title

      Journal of Veterinary Medical Science (In Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Involvement of coactivator CBP in the regulation of conceptus interferon?gene expression.2003

    • Author(s)
      Xu, others.
    • Journal Title

      Molecular Reproduction and Development 65

      Pages: 23-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A chemokine, interferon (IFN)-γ-inducible protein, is stimulated by IFN-τ and recruits immune cells in the ovine endometrium.2003

    • Author(s)
      Nagaoka, others.
    • Journal Title

      Biology of Reproduction 68

      Pages: 1413-1421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Regulation of endometrial granulocyte macrophage colony stimulating factor (GM-CSF) in the ewe2002

    • Author(s)
      McGuire, その他
    • Journal Title

      Domestic Animal Endocrinology 23

      Pages: 383-396

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Establishment of feeder-independent cloned caprine trophoblast cell line which Expresses placental lactogen and interferon-tau2002

    • Author(s)
      Miyazaki, その他
    • Journal Title

      Placenta 23

      Pages: 613-630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Regulation of endometrial granulocyte macrophage colony stimulating factor (GM-CSF) in the ewe.2002

    • Author(s)
      McGuire, others.
    • Journal Title

      Domestic Animal Endocrinology 23

      Pages: 383-396

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Establishment of feeder?independent cloned caprine trophoblast cell line which expresses placental lactogen and interferon-tau.2002

    • Author(s)
      Miyazaki, others.
    • Journal Title

      Placenta 23

      Pages: 613-630

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Reactivation of feline foamy virus from a chronically infected feline renal cell line by tricostatin A.2001

    • Author(s)
      Hatama, others.
    • Journal Title

      Virology 283

      Pages: 315-323

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] 生殖科学(佐藤英明編)2003

    • Author(s)
      今川和彦
    • Total Pages
      250
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Patent(Industrial Property Rights)] IP-10による着床制御2002

    • Inventor(s)
      今川和彦, 永岡謙太郎, 渡辺史子
    • Industrial Property Rights Holder
      プロテインエクスプレス
    • Industrial Property Number
      2002-259268
    • Filing Date
      2002
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujii, その他: "Inhibition of HIV-1 infection to human T-cell line by IFN-tau gene expression transduced with human spumavirus"Biochemical and biophysical research communications. (In revision). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Xu, その他: "Involvement of coactivactor CBP in the regulation of conceptus IFNτ gene expression"Molecular Reproduction and Development. (In Press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagaoka, その他: "Expression of a chemokine, interferon-gamma-inducible protein 10 kDa, is stimulated by IFN-τ in the ovine endometrium"Biology of Reproduction. (In Press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suda, その他: "Long-term selection for small body weight in Japanese quail : Direct selection response from 60 to 65th generations"Journal of Poultry Science. 39. 274-284 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suda, その他: "Prediction of pregnancy in Shiba goats : mathematical analysis using plasma amino acid concentrations"Journal of Reproduction and Development. 48. 523-529 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] McGuire, その他: "Regulation of endometrial granulocyte macropharge colony stimulating factor (GM-CSF) in the ewe"Domestic Animal Endocrinology. 23. 383-396 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 今川 和彦: "生殖科学(佐藤英明 編)"朝倉書店. 250 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaguchi その他: "Enhancer regions of ovine interferon-tau gene that confer PMA response or cell Type specific transcription"Molecular and Cellular Endocrinology. 173. 147-155 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hatama その他: "Reactivation of feline foamy virus from a chronically infected feline renal cell line By Tricostatin A"Virology. 283. 315-323 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 今井和彦, その他: "着床期におけるサイトカイン遺伝子の発現:New and old approaches"日本受精着床学会雑誌. 18. 38-41 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi, その他: "Regulation of inteferon-tau gene expression and maternal recognition of Pregnancy-Review"Journal of Reproduction and Development. 47. 69-82 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 今川和彦 他: "妊娠の生物学 着床とインターフェロン関連遺伝子"永井書店. 396 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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