Metabolic engineering of acetyle-CoA carboxylase
| Project/Area Number |
13556059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Saga University (2002)
Nagoya University (2001) |
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Principal Investigator |
NAGANO Yukio (2002) Saga University, Analytical Research Center for Experimental Sciences, Associate Professor, 総合分析実験センター, 助教授 (00263038)
佐々木 幸子 (2001) 名古屋大学, 大学院・生命農学研究科, 教授 (00026519)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Yukiko Genesis Research Institute, INC., Executive Research Scientist, 特別研究員
永野 幸生 名古屋大学, 大学院・生命農学研究科, 助手 (00263038)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | fatty acid synthesis / acetyl-CoA carboxylase / metabolic engineering / transgenic plants / seed oil / plastid transformation / tobacco / seed yield |
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| Research Abstract |
Acetyl-CoA carboxylase (ACCase) in plastids is a key enzyme regulating the rate of de novo fatty acid biosynthesis in plants. Plastidic ACCase is composed of three nuclear-encoded subunits and one plastid-encoded accD subunit. To boost ACCase levels, we examined whether overexpression of accD elevates ACCase production. Using homologous recombination, we replaced the promoter of the accD operon in the tobacco plastid genome with a plastid rRNA-operon (rrn) promoter that directs enhanced expression in photosynthetic and non-photosynthetic organs, and successfully raised the total ACCase levels in plastids. This result suggests that the level of the accD subunit is a determinant of ACCase levels, and that enzyme levels are in part controlled post-transcriptionally at the level of subunit assembly. The resultant transformants grew normally and the fatty acid content was significantly increased in leaves, but not significantly in seeds. However, the transformants displayed extended Leaf longevity and a twofold increase of seed yield over the control value, which eventually almost doubled the fatty acid production per plant of the transformants relative to control and wild-type plants. These findings offer a potential method for raising plant productivity and oil production.
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Report
(3 results)
Research Products
(7 results)
