| Project/Area Number |
13557032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Public health/Health science
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| Research Institution | Gunma University |
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Principal Investigator |
KOYAMA Hiroshi Gunma University, Professor, 大学院・医学系研究科, 教授 (30143192)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Shosuke Gunma Occupational Health Promotion Center, Director, センター長(研究職) (40010011)
HONGO Tetsuro Yamanashi Institute of Environmental Sciences, Chief Researcher, 環境保健研究部, 主幹研究員 (90199563)
SASADA Yoko Morioka University, Professor, 短期大学部, 教授 (30258769)
MIYAZAKI Yukiko Jobu University, Professor, 看護学部, 教授 (00251190)
NOJIRI Masami Chiba University, Professor, 看護学部, 教授 (70009520)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2001: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | selenium / speciation analysis / glutathione peroxidase / selenoprotein P / albumin / food frequency questionare / グルタチオンペルオキシターゼ / アルプミン / 簡易食品摂取頻度調査票 / 簡易食事接種頻度調査票 / セレン化学形別分析 / KKマウス |
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| Research Abstract |
To analyze which of the three selenium-containing proteins (albumin, glutathione peroxidase(GPx) and selenoprotein P) was responsible for the development of stroke. We conducted a population-based case-control study of 30 first-ever stroke cases who had undergone health examinations and blood sampling in 1992 to 1994 before the stroke, and 30 controls matched for their sex, age, total cholesterol, and the year of blood sampling. Total selenium in the serum was measured fluorometrically. Selenium-containing proteins were separated using two HPLC columns in tandem, and selenium was detected by ICP-MS.
The serum concentration-of selenium tended to be lower in the stroke cases than in the controls (105.2 vs 116.5 μg/l;p=0.054). Although the selenium contents in GPx and albumin did not show any significant difference, selenoprotein P was significantly lower in the stroke cases than in the controls (54.5 vs 63.0 μg/l;p=0.006). A multiple logistic analysis showed that selenoprotein P can reduce the risk of stroke (Odds Ratio=0.28;95% Confidence Interval:0.10-0.8).
The results of this study show that Selenoprotein P may play an important role in development of stroke.
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