| Project/Area Number |
13557049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Gastroenterology
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| Research Institution | Hiroshima University |
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Principal Investigator |
CHAYAMA Kazuaki Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00211376)
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| Project Period (FY) |
2001 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Keywords | hepatitis B virus / HBs antigen / HBe antigen / HBV DNA / replication / core promoter / adefovir / lamivudine / YMDDvariant / HBV / real time PCR / whole genome / mutation / 薬剤耐性 |
|---|
| Research Abstract |
Hepatitis B virus infection is a serious health problem worldwide. As the hepatitis B virus infects only humans and chimpanzees, establishment of reliable culture system is necessary to study this important pathogen. We cloned a fu11 length genotype C clone of the hepatitis B virus from a patient with a very mild hepatitis. About one and half genome was cloned into a plasmid vector. The 1.4 length clone was transfected to a HepG2 cell line. The resultant cell line produced HBs antigen, HBe antigen and HBV DNA to a supernatant. The titer of HBV DNA was 10^6-10^7 copy/ml. The production of the HBV DNA was reduced by incubation of lamivudine and interferons. The clones should be useful to further develop anti-hepatitis B virus agents and study biological properties of the hepatitis B virus.
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