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The establishment of diagnostic system for all the autoimmune bullous disease using various biochemical techniques

Research Project

Project/Area Number 13557072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Dermatology
Research InstitutionKURUME UNIVERSITY

Principal Investigator

HASHIMOTO Takashi  Kurume University, Dermotol, Professor, 医学部, 教授 (20129597)

Co-Investigator(Kenkyū-buntansha) NAKAMA Takekuni  Kurume University, Dermotol, As Prof, 講師 (50221453)
YASUMOTO Shinichiro  Kurume University, Dermotol, As Prof, 助教授 (10220162)
MORI Osamu  Kurume University, Dermotol, As Prof, 助教授 (10175630)
INOUE Mitsuse  Kurume University, Dermotol, As Prof, 講師 (10232563)
楠原 正洋  久留米大学, 医学部, 講師 (40195441)
橋本 隆  久留米大学, 医学部, 教授 (20129597)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsAutoimmune disease / Pemphigus / Bullous pemphigoid / Recombinant protein / Immunoblotting / ELISA / Desmosome / Hemidesmosome / ケラチノサイト / ヘミデスモソーム / 類天疱瘡 / ELISA法 / 免疫電顕 / 皮膚自己抗原 / ラミニン5 / 類天疱瘡抗原 / 自己免疫性水泡症
Research Abstract

We isolate cDNA of desmocollin (Dsc) 1-3, and produced baculoproteins of Dsc1-3 using vaculovirus expression system. Using these recombinant protein, we established ELISA of Dsc. We produced bacterial recombiant proteins of the 3 different domains of envoplakin and periplakin using pGEX expression system, and detected autoantibodies in paraneoplastic pemphigus sera by immunoblotting using the recombinant proteins. We also produced recombinant proteins of 3 different domains of BP230, and detected autoantibodies in bullous pemphigoid by immunoblotting using the proteins. We produced recombinant proteins of various domains of BP18O, and examined the reactivity of cicatricial pemphigoid by immunoblotting using the recombinant proteins. We established immunoblotting using laminin 5 purified from the keratinocyte cultures and detected autoantibodies in the sera of anti-laminin 5 cicatricial pemphigoid. We established immunoblotting using concentrated culture supernatant of keratinocytes, and detected antibodies against LAD1 in LAD sera. We produced pGEX recombinant proteins of NC1 and NC2 domains of type VII collagen, and found that most EBA sera reacted with NC1 domain, and a few EBA sera reacted with NC2 domain.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Egan CA et al.: "IgG anti-LABD97 an antibodies in bullous pemphigoid patients' sera react with the mid-portion of the BPAg2 ectodomain."J Invest Dermatol. 116. 348-350 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata Y et al.: "Paraneoplastic pemphigus sera react strongly with multiple epitopes on the various regions of envoplakin and periplakin, except for the C-terminal homologous domain of periplakin."J Invest Dermatol. 116. 556-563 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto T et al.: "Detection of IgA autoantibodies to desmogleins by an enzyme-linked immunosorbent assay -The presence of new minor subtypes of IgA pemphigus"Arch Dermatol. 137. 735-738 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sitaru C et al.: "Autoantibodies to type VII collagen mediate Fc -dependent neutrophil activation and induce dermal-epidermal separation in cryosections of human skin"Am J Pathol. 161. 301-311 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimanovich I et al.: "The autoantigen of anti-p200 pemphigoid is an acidic noncollagenous N-linked glycoprotein of the cutaneous basement membrane"J Invest Dermatol. 121. 1402-1408 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 橋本 隆: "図解皮膚科学テキスト"中外医学社. 235 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Egan CA et al.: "IgG anti-LABD97 antibodies in bullous pemphigoid patients sera react with the mid-portion of the BPAg2 ectodomain."J Invest Dermatol. 116. 348-350 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata Y et al.: "Paraneoplastic pemphigus sera react strongly with multiple epitopes on the various regions of envoplakin and periplakin, except for C-terminal homologous domain of periplakin"J Invest Dermatol. 116. 556-563 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto T et al.: "Detection of IgA autoantibodies to desmogleins by an enzyme-lined immunosorbent assay -The presence of new minor subtypes of IgA pemphigus"Arch Dermatol. 137. 735-738 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sitaru C et al.: "Autoantibodies to type VII collagen mediate Fcg-dependent neutrophilic activation and induce dermal-epidermal separation in cryosections of human skin."Am J Pathol. 161. 301-311 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimanovich I et al.: "The autoantigen of anti-p200 pemphigoid is an acidic noncollagenous N-linked glycoprotein of the cutaneous basement membrane"J Invest Dermatol.. 121. 1402-1408 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Lee JB, Liu Y, Hashimoto T: "Cicatricial pemphigoid sera specifically react with the most C-terminal portion of BP180"J Dermatol Sci. 32(1). 59-64 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Liu Y, Shimizu H, Hashimoto T: "Immunofluorescence studies using skin section of recessive dystrophic epidermolysis bullosa patients indicated that the antigen of anti-p200 pemphigoid is not a fragment of type VII collagen"J Dermatol Sci. 32(2). 125-129 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hisamatsu Y, Nishiyama T, Amano S, Matsui C, Ghohestani: "Usefulness of immunoblotting using purified laminin 5 in the diagnosis of anti-laminin 5 cicatricial pemphigoid"J Dermatol Sci. 33(2). 113-119 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Futei Y, Amagai M, Hashimoto T, Nishikawa T: "Conformational epitope mapping and IgG subclass distribution of desmoglein 3 in paraneoplastic pemphigus"J Am Acad Dermatol. 49(6). 1023-1028 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimanovich I, Hirako Y, Sitaru C, Hashimoto T, Brocker: "The autoantigen of anti-p200 pemphigoid is an acidic noncollagenous N-linked glycoprotein of the cutaneous basement membrane"J Invest Dermatol. 121(6). 1402-1408 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishii N, Ishida-Yamamoto A, Hashimoto T: "Immunolocalization of target autoantigens in IgA pemphigus"Clin Exp Dermatol. 29(1). 62-66 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 橋本 隆: "図解皮膚科学テキスト"中外医学社. 235 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Leverkus M, Hashimoto T et al.: "Cicatricial pephigoid with circulating IgA and IgG antibodies to the central portion of the BP180 ectodomain. Beneficial effect of adjuvant therapy with high dose intravenous immunoglobulin"J Am Acad Dermatol. 46. 116-122 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sitaru C, Hashimoto T et al.: "Autoantibodies to type VII collagen mediate Fcg-dependent neutrophilic activation and and induce dermal-epidermal separation in cryosections of human skin"Am J Pathol. 161. 301-311 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimanovich I, Hashimoto T et al.: "Pemphigoid gestationis with predominant involvement of oral mucous membranes and IgA autoantibodies targeting the C-terminus of BP180"J Am Acad Dermatol. 47. 780-784 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayashi M, Hashiomoto T et al.: "BP180 ELISA as a diagnostic and moritoring tool for bullous pemphigoid"J Dermatol Sci. 30(3). 224-232 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oiso N, Hashimoto T et al.: "IgG/IgA pemphigus with IgA and IgG anti-Dsgl autoantibodies detected by enzyme-linked immunosorbent assay"Br J Dermatol. 147. 1012-1017 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hash KS, Hashimoto T et al.: "Aggressive immunosuppressive therapy for a refractory case of IgA pemphigus"Arch Dermatol. 138. 744-746 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 橋本 隆: "今日の皮膚疾患治療指針"医学書院(編集:齊田俊明、塩原哲夫、宮地良樹、渡辺晋一). 789 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagata Y, Hashimoto T et al.: "Paraneoplastic pemphigus sera react strongly with multiple epitopes on the various regions of envoplakin and periplakin, except for C-terminal homologous domain of periplakin"J Invest Dermatol. 116. 556-563 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto T et al.: "Detection of IgA autoantibodies to desmogleins by an enzyme-lined immunosorbent assay-The presence of new minor subtypes of IgA pemphigus"Arch Dermatol. 137. 735-738 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hamada T, HashimotoT et al.: "Bullous pemphigoid sera react specifically With various domains of BP230, most frequently with C-terminal domain, by immunoblot analyses using bacterial recombinant proteins covering the entire molecule"Exp Dermatol. 10. 256-263 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Komai A, Hashimoto T et al.: "The clinical transition between pemphigus foliaceus and pemphigus vulgaris correlates well with the changes in autoantibody profile assessed by an enzyme-linked immunosorbent assay"Br J Dermatol. 144. 1177-1182 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto T: "Immunopathology of paraneoplastic pemphigus"Clin Dermatol. 19. 675-682 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto T: "Immunopathology of IgA pemphigus"Clin Dermatol. 19. 683-689 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 橋本 隆: "皮膚科専門医 Clinical Exercises、第一版、(宮地良樹、橋本 隆、編集)東京"医学書院. 182 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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