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Regeneration of mandibular bone by the implantation of cell fixed artificial periostium

Research Project

Project/Area Number 13557186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Periodontal dentistry
Research InstitutionHokkaido University

Principal Investigator

MIZUNO Morimichi  Hokkaido Univ., Grad. School of Dent, Assist. Prof., 大学院・歯学研究科, 助手 (10125354)

Co-Investigator(Kenkyū-buntansha) KOMORI Takahide  Kobe Univ., Grad. School of Medicine, Professor., 大学院・医学研究科, 教授 (50251294)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2002: ¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2001: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordsartificial periostium / osteoblastic differentiation / bone formation / 顎骨 / 再生 / 骨芽細胞 / 骨髄細胞 / コラーゲン
Research Abstract

So far, the injection of growth factor, and implantation of cells have been attempted to regenerate bone ; however, the bone mass formed by these treatments is little. In this study, I tried to develop new artificial periostium constituted by cells, growth factors and carrier, and investigated the optimal condition for the regeneration of bone in aged peoples. First of all, I looked for the factors that the artificial periostium should have. I harvested bone marrow cells to fix into artificial periostium. They possess the ability to differentiate into osteoblasts, and this differentiation is induced by the treatment of cells by dexamethasone, but dexamethasone is a kind of hormone, and is hard to use the clinical treatment. It has been reported that type I collagen has an ability to induce the osteobrastic differentiation of bone marrow cells, and I studied whether type I collagen induced this kind of differentiation. Osteocalcin and bone sialoprotein gene are osteoblast-specific one, and I found that bone marrow cells cultured in type I collagen gel showed high expression of these genes. Next I implanted the artificial periostium in rats, and found it formed bone in vivo. These results indicate that the artificial periostum we constructed has an ability to form bone, and I clould conclude that the artificial periostum is a potent tool to regenerate the mandibular bone.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Mizuno, M: "Type I Collagen Regulated Dentin Matrix Protein 1 and Osteocalcin Gene Expression of Rat Dental Pulp Cells"Journal of Cellular Biochemistry. 88. 1112-1119 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ojima Y.: "In vitro effect of platelet-derived growth factor-BB on collagen synthesis and proliferation of human periodontal ligament cells"Oral Disease. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizuno M.: "Type I collagen regulated dentin matrix protein 1 and osteocalcin gene of rat dental pulp cells"Journal of Cellular Biochemistry. 88. 1112-1119 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ojima Y.: "In vitro effect of platelet-derived growth factor-BB on collagen synthesis and proliferation of periodontal ligament cells"Oral Disease. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizuno M.: "Type I Collagen Regulated Dentin Matrix Protein I and Osteocalcin Gene Expression of Dental Pulp Cells"Journal of Cellular Biochemistry. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ojima Y.: "In Vitro Effect of Platelet-derived Growth Factor-BB on Collagen Synthesis and Proliferation of Human Periodontal Ligament Cells"Oral Desease. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuno, M.: "Osfeoblost-related gene expression of bone marrow cells during the osfeoblosti ditterenfiation induced by type I collagen"Journal of Biochemistry. 129. 133-138 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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