| Project/Area Number |
13557192
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
NISHIHARA Tatsuji Kyushu Dental College Department of Oral Microbiology Professor, 歯学部, 教授 (80192251)
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| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Akihiro Seikagaku Corporation Tokyo Research Institute Manager, 中央研究所, 研究員 (60135530)
KAWAHARA Hiroharu Kitakyushu National College of Technology Department of Material Science and Chemical Engineering Assistant Professor, 生物化学工業, 助教授 (20321515)
TUJISAWA Toshiyuki Kyushu Dental College Department of Oral Microbiology Assistant, 歯学部, 助手 (60265006)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 2002: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2001: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | human monoclonal antibody / periodontitis / inflammatory bone resorption / inflammatory cytokine / RANKL / osteoclast / electroporation / alveolar bone resorption / 炎症性骨吸収 / 歯周病 / 炎症性サイトカイン / インターロイキン-1 |
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| Research Abstract |
Osteoclasts, bone resorbing multinucleated cells, develop from monocyte-macrophage lineage cells in the presence of receptor activator NF-kB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). M-CSF-dependent bone marrow macrophages from mouse bone marrow cells have been shown to differentiate into osteoclast-like multinucleated cells (OCLs) in the presence of soluble RANKL and M-CSF. To investigate human biological responses, it is better to use human-derived proteins, such as human recombinant proteins and monoclonal antibodies. In the present study, we established human hybridomas secreting antibodies against RANKL using in vivo immunization of peripheral blood lymphocytes, and obtained some positive clones. We found that human anti-RANKL monoclonal antibody partially suppress the formation of OCL in vitro. Next, we tried to establish a method of in vivo electroporation to control the inflammatory bone resorption using human anti-RANKL antibody. However, we have no positive results in this study. Further work is needed to put a human monoclonal antibody against RANKL to practical use. Taken together, these results suggest that human monoclonal antibody against RANKL may be useful to control the inflammatory bone resorption in periodontitis.
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