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Development of cold-spray ionization mass spectrometry and its application to supramolecules and biomolecules

Research Project

Project/Area Number 13557198
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Physical pharmacy
Research InstitutionChiba University

Principal Investigator

YAMAGUCHI Kentaro  Chemical Analysis Center, Associate Professor, 分析センター, 助教授 (50159208)

Co-Investigator(Kenkyū-buntansha) SEKI Hiroko  Chemical Analysis Center, Research Associate, 分析センター, 助手 (60114245)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥9,600,000 (Direct Cost: ¥9,600,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywordscold-spray / DNA / cluster ion / nucleoside / hydrogen bonding / electrospray / mass spectrometry / ionization / ヌクレオシド / コールドスプレーイオン化 / 生体分子 / クラスターイオン / 非共有性相互作用
Research Abstract

A direct solution analysis method, cold-spray ionization (CSI) mass spectrometry (MS), a variant of electrospray (ESI) MS operating at low temperature (ca. -80 〜 10℃), allows facile and precise characterization of labile organic species, especially those in which non-covalent bonding interactions are prominent. We have applied this method to investigations of the solution structures of many labile organic species, including unstable reagents and reaction intermediates, asymmetric catalysts, supramolecules, and even primary biomolecules. Remarkable analytical results were obtained for highly ordered supramolecules using the CSI method. While conventional ESI is not applicable to these compounds because of their instability to heat and/or air, CSI affords multiply charged molecular ions with many solvent molecules attached. Investigation of the constitution of Grignard reagents in solution is extremely challenging, but CSI-MS allowed us to identify one of the key structures in THF solution. Recently, this method was adopted for investigations of the solution structures of primary biomolecules such as nucleosides, amino acids, sugars and lipids, revealing singly charged Na^+ adducts of large clusters (chain structures), presumably linked by non-covalent interactions, including hydrogen bonding and/or hydrophobic interactions.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 國村美希, 坂本 茂, 山口健太郎: "Alkali Metal-Mediated Proline Aggregation in Solution Observed by Coldspray Ionization Mass Spectrometry"Organic Letters. 7. 347-350 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 坂本 茂, 中谷和彦, 齋藤 烈, 山口健太郎: "Formation and destruction of the guanine quartet solution observed by CSI-MS"Chem.Commun.. 788-789 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 坂本 茂, 山口健太郎: "Low Tm DNA duplexes observation by cold-spray ionization mass spectrometry"Tetrahedron Letters. 44. 3341-3344 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 坂本 茂, 山口健太郎: "Hyper-Stranded DNA Architecture Observed by Coldspray lonization Mass Spectrometry"Angew.Chem.Int.Ed.. 42. 905-908 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山口 健太郎: "Cold-Spray Ionization Mass Spectrometry : Principle and Applications"Journal of Mass Spectrometry. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Kunimura, S. Sakamoto, K. Yamaguchi: "Alkali Metal-Mediated Proline Aggregation in Solution Observed by Coldspray Ionization Mass Spectrometry"Organic Letters. 4(3). 347-350 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.-M. Park, S.-Y. Kim, J. Heo, Dongm. Whang, S. Sakamoto, K. Yamaguchi, K. Kim: "Designed Self-Assembly of Molecular Necklaces"J. Am. Chem. Soc.. 124(10). 2140-2147 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Kubota, S. Sakamoto, K. Yamaguchi, M. Fujita: "Guest-induced organization of an optimal receptor from a dynamic receptor library: Spectroscopic screening"PRNS. 99 (No.8). 4854-4856 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuda, T. Nakamura, S. Sakamoto, K. Yamaguchi, A. OsukaA: "self-Assembled Porphyrin Box from meso-meso linked Bis(5-/?-pyridyl-15-(3,5- dioctyIoxphenyl)Zn^<II>-porphyrin)"Angew. Chem. Int. Ed.. 41, No. 15. 2817-2821 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Hori, A. Akasaka, K. Birada, S. Sakamoto, K. Yamaguchi, M. Fujita: "Chiraliry Induction through the Reversible Vatenation of Coordination Rings"Angew. Chem. Int. Ed.. 41, No. 17. 3269-3272 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Nakatani, S. Hagihara, S. Sando, S. Sakamoto, K. Yamaguchi, C. Maesawa, I. Saito: "Induction of a Remarkable Conformational Change in a Human Telomeric Sequence by the Binding of Naphthyridine Dimer : Inhibition of the Elongation of a Telomeric Repeat by Telomerase"J. Am. Chem. Soc.. 125 (3). 662-666 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Ochiai, T. Suefuji, K. Miyamoto, N. Tada, S. Goto, M. Shiro, S. Sakamoto, K. Yamaguchi: "Secondary Hypervalent IP I(III)―O Interactions : Synthesis and Structure of Hypervalent Complexes of Diphenyl-3-iodanes with 18-Crown-6"J. Am. Chem. Soc.. 125 (3). 769-773 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Sakamoto, K. Yamaguchi: "Hyper-Stranded DNA Architecture Observed by Coldspray lonization Mass Spectrometry"Angew. Chem. Int. Ed.. 42, No. 8. 905-908 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Sakamoto, K. Nakatani, I. Saito, K. Yamaguchi: "Formation and destruction of the guanine quartet in solution observed by coldspray ionization mass Spectrometry"Chem. Commun.. 788-789 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Sakamoto, K, Yamaguchi: "Low TM DNA duplexes observed by cold-spray ionization mass Spectrometry"Tetrahedron Letters. 44. 3341-3344 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 國村美希, 坂本 茂, 山口健太郎: "Alkali Metal-Mediated Proline Aggregation in Solution Observed By Coldspray Ionization Mass Spectrometry"Organic Letters. 7. 347-350 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 坂本 茂, 中谷和彦, 齋藤 烈, 山口健太郎: "Formation and destruction of the guanine quartet solution observed by CSI-MS"Chem.Commun.. 788-789 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shigeru Sakamoto: "Characterization of Encapsulated Supramolecules by Using CSI-MS with Ionization Promoting Reagents"Organic Letters. 3・11. 1601-1604 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miki Kunimura: "Alkali metal-Mediated Proline Aggregation in Solution Observed by Coldspray Ionization Mass Spectrometry"Organic Letters. 4・3. 347-350 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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