| Project/Area Number |
13557206
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biological pharmacy
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
IDE Toshinori Hiroshima University, Graduated School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (60012746)
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|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥8,700,000 (Direct Cost: ¥8,700,000)
|
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| Keywords | human somatic cell / immortalization / telomere / telomerase / proliferative life span / endothelial cell / astrocyte / hepatocyte |
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| Research Abstract |
Such human somatic cells as fibroblasts, umbirical vein endothelial cells, astrocytes, and hepatocytes were immortalized by an introduction with human telomerase reverse transcriptase (hTERT) gene. Only an exception was osteoblast which could not be extended proliferative lifespan at all by hTERT introduction. All hTERT-introduced immortal cells showed normal phenotypes. Immortalized hepatocytes expressed bile duct specific markers in addition to hepatocyte specific markers, suggesting characteristics of stem cells which are able to differentiate into both hepatocytes and bile duct cells.
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