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Establishment of aspiration biopsy RNA diagnosis of malignant lymphoma

Research Project

Project/Area Number 13557227
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory medicine
Research InstitutionOsaka University

Principal Investigator

TAKANO Toru  Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (00263236)

Co-Investigator(Kenkyū-buntansha) NOTOMI Tsugunori  Eiken Co., DUG Unit, Head, DUGユニット技術開発チーム, チームリーダー(研究職)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsmRNA / thyroid / lymphoma / PCR / monoclonality / immunoglobulin / aspiration biopsy / DNA / Thyroid / Malignant Lymphoma / ABRP / RT-PCR / IgM / Monoclonality
Research Abstract

Distinguishing between thyroid malignant lymphoma and lymphocytic thyroiditis (Hashimoto's tbyroiditis) is 'quite difficult and problematic. Molecular techniques to detect clonal lymphoid proliferation based on immunoglobulin heavy chain (IgH) gene rearrangement may be used to facilitate more accurate diagnosis of malignant lymphoma. We tried to establish a method for diagnosing thyroid tumors by analyzing nucleic acids extracted from the needles used for fine needle aspiration biopsy. We first studied RNAs from fresh tissues samples of 8 cases of Hashimoto's thyroiditis and 18 malignant lyrnphomas to detect the monoclonality of IgH mRNA by semi-nested R.T-PCR. Monoclonality was detected in 8 of 18 (44.4%) malignant ryrnphomas but none of the 8 cases of Hashimoto's thyroiditis. We then studied aspirates from 10 cases of thyroid malignant lymphoma, 4 cases of Hashimoto's thyroiditis, and one case each of adenomatous goiter and papillary carcinoma. Monoclonality was detected in the aspirates from 4 of 10 malignant lymphomas (40%) but not from other tissues. Thus RT-PCR detection of monoclonality of IgH mRNA in addition to eytological examination may be useful in diagnosing thyroid malignant lyraphoma. In order to increase the sensitivity of this method, we examine the usefulness of degenerated oligonucleotide primed (OOP) PCR to amplify the extracted DNAs in fine needle aspiration biopsies. By mixing a primer specific for the JH sequence, DOP-PCR could produce 3-10kbp PCR product, which could be used in linker-ligation PCR or Southern blot analysis. These results may lead us to establish a low-invasive diagnostic method of malignant lymphoma in the future study by utilizing DNAs instead of RNAs obtained by fine needle aspiration biopsy

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Takano T, et al.: "Cancer-specific mRNAs in thyroid carcinomas : detection, use, and their implication in thyroid carcinogenesis"Endocr J. 49・2. 97-107 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano T, et al.: "Quantitative analysis of thymosin beta-10 messenger RNA in thyroid carcinomas"Jpn J Clin Oncol. 32・7. 229-232 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano T, et al.: "Quantitative analysis of osteonectin mRNA in thyroid carcinomas"Endocr J. 49・4. 511-516 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa Y, Takano T, et al.: "Decreased expression of glutathione peroxidase mRNA in thyroid anaplastic carcinoma"Cancer Lett. 182・1. 69-74 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa Y, Takano T, et al.: "Decreased expression of catalase mRNA in thyroid anaplastic carcinoma"Jpn J Clin Oncol. 33・1. 6-9 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, T, Hasegawa, Y. Miyauchi, A, Matsuzuka, F, Yoshida, H, Kuma, K, Hayashi, N, Nakamori, S, Amino N: "Quantitative analysis of ostecnectin mRNA in thyroid carcinomas"Endocr J. 49-4. 511-516 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, T, Hasegawa, Y, Miyauchi, A, Matsuzuka, F, Yoshida, H, Kuma, K, Amino, N.: "Quantitative analysis of thymosin beta-10 messenger RNA in thyroid carcinomas"Jpn J Clin Onco. 32-7. 229-232 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa, Y, Takano, T, Miyauchi, A, Matsuzuka, F, Yoshida, H, Kuma, K, Amino, N.: "Decreased expression of glutathione peroxidase mRNA in thyroid anaplastic carcinoma"Cancer Lett. 182-1. 69-74 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, T, Amino, N.: "Cancer-specific mRNAs in thyroid carcinomas detection, use, and their implication in thyroid carcinogenesis"Endocr J. 49-2. 97-107 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa, Y, Takano, T, Miyauchi, A, Matsuzuka, F, Yoshida, H, Kuma, K, Amino, N.: "Decreased expression of cataiase mRNA in thyroid anaplastic carcinoma"Jpn J Clin Oncol. 33-1. 6-9 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano T, et al.: "Cancer-specific mRNAs in thyroid carcinomas : detection, use, and their implication in thyroid carcinogenesis"Endocr J. 49・2. 97-107 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano T, et al.: "Quantitative analysis of thymosin beta-10 messenger RNA in thyroid carcinomas"Jpn J Clin Oncol. 32・7. 229-232 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano T, et al.: "Quantitative analysis of osteonectin mRNA in thyroid carcinomas"Endocr J. 49・4. 511-516 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hasegawa Y, Takano T, et al.: "Decreased expression of glutathione peroxidase mRNA in thyroid anaplastic carcinoma"Cancer Lett. 182・1. 69-74 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hasegawa Y, Takano T, et al.: "Decreased expression of catalase mRNA in thyroid anaplastic carcinoma"Jpn J Clin Oncol. 33・1. 6-9 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano T et al.: "Cancer-specific mRNA in thyroid caranomas : Detection, use, and their implication in thyroid carcinogenesis"Endocrine Journal. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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