| Project/Area Number |
13558072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
環境保全
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| Research Institution | Niigata University of Pharmacy and Applied Life Sciences (2002)
The University of Tokyo (2001) |
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Principal Investigator |
FUJII Tomoyuki Niigata University of Pharmacy and Applied Life Sciences, Faculty of Applied Life Sciences, Associate Professor, 応用生命科学部, 助教授 (40228953)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAWAKI Osato The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (80012053)
MIYAKE Noriko Niigata University of Pharmacy and Applied Life Sciences, Faculty of Applied Life Sciences, Associate Professor, 応用生命科学部, 助教授 (70314573)
HATTORI Yoshio Niigata University of Pharmacy and Applied Life Sciences, Faculty of Applied Life Sciences, Professor, 応用生命科学部, 教授 (40350714)
TANAKA Takaaki Niigata University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (00217043)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | nanospace / fractal / nanoporous silica membrane / laccase / endocrine disrupting chemical / supercritical carbon dioxide / lipase / critical phenomena / silica membrane |
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| Research Abstract |
A thin nanoporous silica membrane was prepared by the two-step sol-gel method for the purpose that the generation of an aggregate having the higher fractal dimension was suppressed in the intermediate process. It succeeded in the preparation of the thin silica film with nanospace of about 3.3nm. The model reaction in which sea sand was made to adsorb the alkylphenol was carried out, and it was found that adsorbing nonylphenol and octylphenol and bisphenol A are quickly decomposed by laccase. The decomposition of an artificial estrogen also confirmed being possible. Log P, which was an useful parameter when we evaluate the solvent property of supercritical carbon dioxide, was estimated. As the pressure of carbon dioxide was increase on the pressure of 3.0 11.8 MPa, log P tended to increase from 0.9 to 2.0. It was shown that the polarity had appeared in carbon dioxide under the supercritical state by the pressure, and that the polarity became small with the increase in the pressure. On the esterification reaction between stearic acid and ethanol using lipase, the reaction rate rose with the increase in the pressure, and it agreed with the increase tendency of log P approximately. It was shown from these results that the solvent property as a bulk had to be considered in addition to the interaction between solute and carbon dioxide, enzyme surface, wall surface, respectively, when it examined the enzyme reaction in nanospace by using supercritical carbon dioxide as a solvent.
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