• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of 96-type and multi-type quartz-crystal microbalance

Research Project

Project/Area Number 13558079
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Bioorganic chemistry
Research InstitutionTokyo Institute of Technology

Principal Investigator

OKAHATA Yoshio  Tokyo Institute of Technology, Professor, 大学院・生命理工学研究科, 教授 (80038017)

Co-Investigator(Kenkyū-buntansha) FURUSAWA Hiroyuki  Tokyo Institute of Technology, Research Associate, 大学院・生命理工学研究科, 助手 (60345395)
KAWASAKI Takayoshi  Tokyo Institute of Technology, Research Associate, 大学院・生命理工学研究科, 助手 (60334504)
MORI Toshiaki  Tokyo Institute of Technology, Associate Professor, 大学院・生命理工学研究科, 助教授 (50262308)
実川 友史  (株)イニシアム, 主任研究員
長谷川 幸雄  ファルマシアバイオテク(株), 研究開発室, 室長
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2002: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2001: ¥8,700,000 (Direct Cost: ¥8,700,000)
KeywordsDNA hybridization / quartz-crystal microbalance / multi-sensor / 4-hole type QCM / QCM / DNA / たんぱく質
Research Abstract

The purpose of this research is to develop a multi-type (96 hole)quartz-crystal microbalance (QCM In this paper, we introduce a 27-MHz quartz-crystal microbalance (QCM) as a new tool to detect DNA-protein/peptide interactions in an aqueous buffer solution. From the time course of the frequency decrease (mass increase), the binding amount (Δm), association constant (Ka), binding and dissociation rate constant (k1 and k1) can be obtained. When dsDNA was immobilized on a QCM, binding kinetics of antibiotics, a Zinc-finger type peptide, a restriction enzyme, and a polymerase to DNA strands could be obtained in aqueous buffer solution. The 27-MHz QCM is highly sensitive and quantitative enough to detect various molecular interactions on DNA double strands.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] S.Fukusho, H.Furusawa, Y.Okahata: "In vitro Selection and Evaluation of RNA Aptamers that recognize Arginine-rich motif Model Peptide on a Quartz-crystal Microbalance"Chem.Commun.. 88-89 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Mori, M.Li, A.Kobayashi, Y.Okahata: "Reversible Control of Enzymatic Transglycosylations in Supercritical Fluoroform using a Lipid-Coated β-D-Galactosidase"J.Am.Chem.Soc.. 124. 1188-1189 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Hoshino, S.Tajima, H.Nakayama, Y.Okahata: "A RNA-aligned Film prepared from a RNA-Lipid Complex"Maromolecular rapid Commun.. 23. 253-255 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Furusawa, Y.Kitamura, N.Hagiwara, T.Turimoto, Y.Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"ChemPhysChem.. No.5. 446-448 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuno, Y.Okahata: "Direct Monitoring of DNA Cleavages catalyzed by an ATP-dependent Deoxyribonuclease on a 27 MHz Quartz-Crystal Microbalance"Chem.Commun. 470-471 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Fukusho, H. Furusawa, and Y. Okahata: "In vitro Selection and Evaluation of RNA Aptamers that recognize Arginine-rich motif Model Peptide on a Quartz-crystal Microbalance"Chem. Commun.. 88-89 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Hoshino, S. Tajima, H. Nakayama, and Y. Okahata: "A RNA-aligned Film prepared from a RNA-Lipid Complex"Macromolecular rapid Commun.. 23. 253-255 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Furusawa, Y. Kitamura, N. Hagiwara, T. Turimoto, and Y. Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"Chem PhysChem.. No.5. 446-448 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Matsuno and Y. Okahata: "Direct Monitoring of DNA Cleavages catalyzed by an ATP-dependent Deoxyribonuclease on a 27 MHz Quartz-Crystal Microbalance"Chem. Commun.. 470-471 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Fukusho, H.Furusawa, Y.Okahata: "In vitro Selection and Evaluation of RNA Aptamers that recognize Arginine-rich motif Model Peptide on a Quartz-crystal Microbalance"Chem. Commun.. 88-89 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Mori, M.Li, A.Kobayashi, Y.Okahata: "Reversible Control of Enzymatic Transglycosylations in Supercritical Fluoroform using a Lipid-Coated β-D-Galactosidase"J. Am. Chem. Soc.. 124. 1188-1189 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Hoshino, S.Tajima, H.Nakayama, Y.Okahata: "A RNA-aligned Film prepared from a RNA-Lipid Complex"Maromolecular rapid Commun.. 23. 253-255 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Furusawa, Y.Kitamura, N.Hagiwara, T.Turimoto, Y.Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"ChemPhysChem.. No.5. 446-448 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Matsuno, Y.Okahata: "Direct Monitoring of DNA Cleavages catalyzed by an ATP-dependent Deoxyribonuclease on a 27 MHz Quartz-Crystal Microbalance"Chem. Commun. 470-471 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Matsuno, K.Niikura, Y.Okahata: "Direct Monitoring and Kinetic Studies of DNA Polymerase Reactions on a DNA-immobilized Quartz-Crystal Microbalance"Chem. Eur. J.. 7. 3305-3312 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Matsuno, H.Furusawa, Y.Okahata: "Kinetic analyses of ATP-dependent deoxiribonuclease (DNase) reactions on a quartz-crystal microbalance"Nucleic Acids Res. Suppl.. 1. 261-262 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Tanaka, A.Mochizuki, T.Motomura, K.Shimura, M.Onishi, Y.Okahata: "In Situ Studies on Protein Adsorption onto a Poly(2-methoxyethylacrylate) Surface by a Quartz Crystal Microbalance"Colloids and Surfaces A : Physicochemical and Engineering Aspects. 193. 145-152 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi