| Project/Area Number |
13558096
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka University |
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Principal Investigator |
FUKUDA Yutaka Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (90028598)
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| Co-Investigator(Kenkyū-buntansha) |
SAWAI Hajime Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20202103)
INOUE Tetsu Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60263282)
MIYOSHI Tomomitsu Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (70314309)
MORI Nozomu National Center for Geriatrics and Gerontology, Department Head, 分子遺伝学研究部, 部長(研究職) (00130394)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | rerinal ganglion cells / SCG10 / axonal regeneration / microtubule / adenovirus / bcl-2 transgenic mice / apoptosis / hippocampal cells / Bcl-2過剰発現マウス |
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| Research Abstract |
CNS neurons of adult mammals hardly regenerate after axonal damage. In this study, in order to promote axonal regeneration of axotomized retinal ganglion cells, we examined the effect of compulsory expression of genes that control microtubules (MTs). As a candidate of such MT controlling protein we chose SCG10. From previous studies SCG10 family is know to be involved in neuronal cytoskelton formation through depolinezatoin of MTs and it plays an important role in neuronal plasticity and axonal regeneration. Here we first constructed a virus vector which specifically expresses SCG10 and infected it into the hippocampal neurons in vitro. Although the gene was successfully introduced into the cells, as assessed by an over-expression of SCG protein, the length of neuronal process and the number of branching points were, unexpectedly reduced as compared with control untransfected neurons. This, it appears that SCG10 alone has suppressing effect for neuronal process extension. Then, we furt
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her tried to see the effect of combined expressions of other genes of SCG10 like MT controlling proteins, RB3, SCLIP and Stathmin. However, we did not succeed to examine the combined effects of these SCG10 like proteins. Furthermore, we tried to isolate retinal ganglion cells from Bcl-2 over-exoressed mice where neuronal apoptosis is inhibited. Again we did not succeed to isolate retinal ganglion cells enough to evaluate their survivability and capacity for axonal regeneration.
In 2004 we examined whether or no N-terminals with specific domains that contribute to the SCG10's function in depolymerization of MTs by comparing the effect of full-length RB3 with N-terminals and truncated RB3 without such N-terminals. As a result we found that the N-terminal domain is essentially important for control of polymerization of MTs. Thus the SCG10 family in general may control the dynamics of MTs using controlling region of the N-terminal domain and through which neural processes and neural shapes may be controlled. Less
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