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Development of in vitro bitter-and sweet-tastes sensors and screening of taste modulators.

Research Project

Project/Area Number 13558114
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionNagoya City University

Principal Investigator

UGAWA Shinya  Nagoya City University, Graduate School of Medical Sciences, Research Associate., 大学院・医学研究科, 助手 (20326135)

Co-Investigator(Kenkyū-buntansha) HIRABAYASHI Yoshifumi  Nagoya City University, Graduate School of Medical Sciences, Assistant Professor., 大学院・医学研究科, 講師 (30181184)
FUJIMORI Osamu  Nagoya City University, Graduate School of Medical Sciences, Associate Professor., 大学院・医学研究科, 助教授 (30128350)
SHIMADA Shoichi  Nagoya City University, Graduate School of Medical Sciences, Professor., 大学院・医学研究科, 教授 (20216063)
UEDA Takashi  Nagoya City University, Graduate School of Medical Sciences, Research Associate., 大学院・医学研究科, 助手 (90244540)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥7,300,000 (Direct Cost: ¥7,300,000)
KeywordsSour receptor / Taste sensation / Cloning / MDEG / Adaptation / Gene / Heteromer / ASIC / 味覚受容体 / アミロライド感受性陽イオンチャンネル / T2Rfamily / Oocyte Electrophysiology / カルシウムイメージング / 味覚修飾物質
Research Abstract

Acid-sensing ion channel-2a (ASIC2a) is an amiloride-blockable proton-gated action channel, probably contribution to sour-taste detection in rat taste cells. To isolate another subtype of the sour-taste receptor, we screened a rat circumvallate papilla cDNA library and identified ASIC2b, an N-terminal splice variant of ASICA2a. Reverse transcription-polymerase chain reaction analyses confirmed the expression of ASIC2b transcripts in the circumvallate papilla and, moreover, demonstrated its expression in the foliate and fungiform papillae. Immunohistochemical analyses revealed that ASIC2b, as well as ASIC2a, was expressed in a subpopulation of taste cells in the circumvallate, foliate, and fungiform papillae, and some of the cells displayed both ASIC2a-and ASIC2b-immunoreactivities. Subsequent co-immunoprecipitation studies with circumvallate papillae extracts indicated that ASIC2b associated with ASIC2a to form assemblies, and, together with our immunohistochemical findings, strongly suggested that both ASIC2 subunits formed heteromeric channels in taste cells in the circumvallate, foliate, and fungiform papillae. Oocyte electro-physiology demonstrated that the ASIC2a/ASIC2b channel generated maximal inward currents at pH 2.0, which is in good agreement with the in vivo pH-sensitivity of rat taste cells, and that the amiloride-sensitivity of the heteromer decreased with decreasing pH and was almost completely abolished at pH 2.0. These findings provide persuasive explanations for the amiloride-insensitivity of acid-induced responses of rat taste cells.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Ugawa S.: "Identification of sour-taste receptor genes."Anat Sci Int.. 78. 205-210 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T., et al.: "Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells."J Neurosci.. 23. 7376-7380 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S., et al.: "Amiloride insensitive currents of the acid sensing ion channel 2a(ASIC2a)/ASIC2b heteromeric sour-taste receptor channel."J Neurosci.. 23. 3616-3622 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T., et al.: "A novel putative M9.2 isoform of V-ATPase expressed in the nervous system."Neuroreport.. 14. 25-30 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S., et al.: "Amiloride-blockable acid-sensing ion channels are leading acid sensors expressed in human nociceptors."J Clin Invest.. 110. 1185-1190 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishida Y., et al.: "Vanilloid receptor subtype-1(VR1) is specifically localized to taste papillae."Brain Res Mol Brain Res.. 107. 17-22 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T: "Diffuse brain injury induces local expression of Na(+)/myo-inositol cotransporter in the rat brain."Mol Brain Res. 86. 63-69 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S: "Cloning of a mouse colonic system B 0+ amino acid transporter related to amino acid absorption in the colon."Am J Physiol. 281. G365-G370 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda H: "Electron microscopic cytochemical studies of anionic sites in the rat spleen."J Vet Med Sci. 63. 287-291 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S: "A single amino acid substitution in MDEG2 specifically alters desensitization of the proton-activated cation current."Neororeport. 12. 2141-2145 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T: "Identification of coding single-nucleotide polymorphisms in human taste receptor genes involving bitter tasting."Biochem Biophys Res Commun. 285. 148-151 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ozeki H: "Suppression of lens stalk cell apoptosis by hyaluronic acid leads to faulty separation of the lens vesicle."Exp Eye Res. 72. 63-70 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T: "Expression of receptor-activity modifying protein (RAMPs) mRNAs in the mouse brain."Mol Brain Res. 93. 36-45 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakata K: "Cloning of a lymphatic peptide/histidine transporter."Biochem J. 356. 53-60 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S: "Cloning and functional expression of ASIC-b2, a splice variant of ASIC-b, that alters pH-sensitivlty of acid-sensing ion channel complex."Neuroreport. 12. 2865-2869 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishiwaki A: "Expression of glia maturation factor during retinal development in the rat."Mol Brain Res. 95. 103-109 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saishin Y: "Retinal fascin: functional nature. subcellular distribution, and chromosomal localization."Invest Ophthalmol Vis Sci. 41. 2087-2095 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Horimoto M: "A novel strategy for cancer therapy by mutated mammalian degenerin gene transfer."Cancer Gene Ther. 7. 1341-1347 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S.: "Identification of sour-taste receptor genes."Ant Sci Int.. 78(4). 205-10 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T., et al.: "Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells."J Neurosci.. 23(19). 7376-80 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S., et al.: "Amiloride-insensitive currents of the acid-sensing ion channel-2a (ASIC2a)/ASIC2b heteromeric sour-taste receptor channel."J Neurosci.. 23(9). 3616-22 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T., et al.: "A novel putative M9.2 isoform of V-ATPase expressed in the nervous system."Neuroreport.. 14(1). 25-30 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] El-Malky M., et al.: "Intraepithelial infiltration of eosinophils and their contribution to the elimination of adult intestinal nematode, Strongyloides venezuelensis in mice."Parasitol Int.. 52(1). 71-9 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S., et al.: "Amiloride-blockable acid-sensing ion channels are leading acid sensors expressed in human nociceptors."J Clin Invest.. 110(8). 1185-90 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishida Y., et al.: "Vanilloid receptor subtype-1 (VR1) is specifically localized to taste papillae."Brain Res Mol Brain Res.. 107(1). 17-22 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maruyama H., et al.: "Strongyloides venezuelensis : longitudinal distribution of adult worms in the host intestine is influenced by mucosal sulfated carbohydrates."Exp Parasitol.. 100(3). 179-85 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aihara N., et al.: "Interacerebral hemorrhage upregulates na(+)/myo-inositol cotransporter in the rat brain."Neurosci Lett.. 327(1). 21-4 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nozaki M., et al.: "Enhanced expression of adhesion molecules of the retinal vascular endothelium in spontaneous diabetic rats."Ophthalmic Res.. 34(3). 158-64 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda T., et al.: "Diffuse brain injury induces local expression of Na+/myo-inositol cotransporter in the rat brain."Brain Res Mol Brain Res.. 86(1-2). 63-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S., et al.: "Characterization of a mouse colonic system B(0+) amino acid transporter related to amino acid absorption in colon."Am J Physiol Gastrointest Liver Physiol.. 281(2). G365-G70 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda H., et al.: "Electro microscopic cytochemical studies of anionic sites in the rat spleen."J Vet Med Sci.. 63(3). 287-91 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa S., et al.: "A single amino acid substitution in MDEG2 specifically alters desensitization of the proton-activated cation current."Neuroreport.. 12(10). 2141-5 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ugawa et al.: "Amiloride-insensitive currents of the acid-sensing ion channel-2a(ASIC2a)/ASIC2b heteromeric sour-taste receptor channel"J Neurosci.. 23(9). 3616-3622 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueda et al.: "Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells"J Neurosci.. 23(19). 7376-7380 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ugawa S., et al.: "Amiloride-Insensitive Currents of the ASIC2a/ASIC2b Heteromeric Sour-Taste Receptor Channel"Journal of Neuroscience. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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