YOKOYAMA Naoaki Obihiro University, Associate Professor, 原虫病研究センター, 助教授 (80301802)
FUJISAKI Kozo Obihiro University, Professor, 原虫病研究センター, 教授 (00292095)
IGARASHI Ikuo Obihiro University, Professor, 原虫病研究センター, 教授 (80159582)
MIKAMI Takeshi Cabinet Office Government of Japan, Commissioner, 食品安全委員会, 委員 (20091506)
INOUE Noboru Obihiro University, Associate Professor, 原虫病研究センター, 助教授 (10271751)
SAKAI Takeo Nihon University, Professor (50147667)
NAGASAWA Hideyuki Obihiro University, Professor (60172524)
KABEYA Hidenori Nihon University, Associate Professor (10318389)
MARUYAMA Soichi Nihon University, Associate Professor (30181829)
|Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥4,600,000 (Direct Cost: ¥4,600,000)
1)Cloning of genes encoding immunodominant antigens of protozoan parasites
In the present study, many genes encoding irnmunodominat antigens of protozoan parasites were identified, cloned and characterized. The main genes are as follows : B.caballi P48, P134;B.equi EMA-1,EMA-2,P82;B.gibsoni P18,P29,P50;N.caninum SAG1,SRS2;T.gondii SAG1,SAG2,SRS1,ROP-1.
2)Development of serodiagnostic methods for detection of antibodies to protozoan parasites
The genes encoding immunodominant antigens were expressed in E.coli or insect cells, and then established the enzyme-linked immunosorbent assay, latex agglutination test, and immunochromatographic test with these recombinant antigens.
3)Development of polymerase chain reactions for detection of protozoan parasites
The primer sets based on genes described above were designed, and established PCR methods for detection of protozoan parasites.
4)Epidemiological investigation of protozoan diseases in China
Blood samples collected from horses, cattle, pigs, sheep, and dogs, m Shinjiang, Jilin, Beijing, Fujian, and Tibet provinces, China, respectively, were examined for the diagnoses of babesiasis, toxoplasmosis, neosporosis, and cryptosrondiosis, and obtained many new findings.