Molecular Epidemiological Study on Human Hereditary Resistant Factors to Malaria
| Project/Area Number |
13576017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Hygiene
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| Research Institution | Kobe University |
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Principal Investigator |
SHIRAKAWA Taku Kobe University, School of Medicine, Associate professor, 医学部, 助教授 (30171044)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Shigeru Osaka University, Graduate School of Literature, Professor, 大学院・文学研究科, 教授 (30087150)
MATSUO Masafumi Kobe University, Graduate School of Medicine, professor, 大学院・医学系研究科, 教授 (10157266)
NISHIYAMA Kaoru Kobe University, School of Medicine, professor, 医学部, 教授 (00150061)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | Malaria / G6PD Deficiency / α-Thalassemia / Southeast Asian Ovalocytosis / Hemoglobin Abnormality / Gene Analysis / G6PD |
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| Research Abstract |
Inherited erythrocyte disorders related to malaria resistance were investigated in some Asian countries.
We analyzed one hundred twenty two samples of the G6PD deficiency with neonatal jaundice in Singapore by MPTP and direct sequencing method. As the result, the mutations in the exon of G6PD gene were identified in 111 of the samples. By contrast, in other 11 samples, such mutations were not identified in the exon, nor in the promoter region.
In Malaysia, G6PD deficiency and SAO were investigated with 300 cord blood samples and the frequency were 3.3% and 4.8%, respectively. Sixty-five patients out of 141 neonatal jaundice patients were diagnosed as G6PD deficiency. Incidence of SAO in patients with distal renal tubular acidosis (dRTA) was significantly higher (81.8%) compared with normal adults (4%). These results indicate that these disorders become to the health problem in Malaysia.
G6PD deficiency, ovalocytosisi, α-thalassemia and β-globin gene abnormality were investigated with cord and peripheral blood samples in 3 different places in Nepal. Kathmandu valley located on the altitude of 1400m is a low malaria endemic area in Nepal. The frequency of α-thalassemia was 20 % and that of other factors were very low in Kathmandu. Japha located on southeast Nepal is a middle malaria endemic area. The frequency of α-thalassemia was 15 % in this area. The majority such as Brahmin, Chhetri, Newar and Mongolian in Nepal live in Kathmandu and Jhapa. On the other hand, Danuwar, a minority in Nepal, living in high malaria endemic area had high prevalence of α-thalassemia (75%) and G6PD deficiency (3.1%). These results suggest that α-thalassemia adapt Danuwar to malaria infection, whereas almost majority in Nepal have little resistant factors to malaria.
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Report
(4 results)
Research Products
(13 results)
