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The mechanisms by which the transition temperature of triple helix to random coil is determined

Research Project

Project/Area Number 13640505
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionNagoya University, Faculty of Science, Dept.of Chemistry

Principal Investigator

TOMIYAMA Tetsuo  Nagoya University, Science, Res.assis., 大学院・理学研究科, 助手 (20155576)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Yuji  Osaka University, Pharmacy, Prof., 大学院・薬学研究科, 教授 (20127228)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywords(Pro-Pro-Gly)_<10> / proline / tansition temperature / conformational change / alcohol / low temperature state / NMR / denature conformation / プロリンリングの挙動 / UPとDOWN / (Pro-Pro-Gly)10 / 溶液内構造 / トリプルヘリックス / 3本鎖 / 溶液内相転移 / イソプロパノール / 部分モル体積
Research Abstract

(1) A collagen model peptide, (Pro-Pro-Gly) 10, forms the triple helix in solution. The triple helix had always been considered to exist as a single state. In this study, two states of the triple helix of (Pro-Pro-Gly) 10 were investigated in detail by NMR. Complete stereospecific assignments of the methylene protons of the proline residues in the triple helix were *ccomplished by the use of NOESY and TOCSY spectra. The temperature dependence of the 1H chemical shifts showed that the thermal transition from the HL to the HH, i.e., the HL-HH thermal transition, can be attributed to the conformational change of the first proline (Pro1) residues.
(2) The effects of deuterated alcohols on the thermal transition of a collagen model peptide, (Pro-Pro-Gly) 10, in D2O solution has been studied in detail by NMR. 1-propanol (d8) and 2-propanol (d8) showed effects quite different from that of methanol (d4) and ethanol (d6) on the thermal transition of (Pro-Pro-Gly) 10 at low alcohol concentration ; These were explained by considering different effects of alcohols on each of the two coexisting conformations. These results indicate in general that the effects of the additives on the denatured conformation which is more susceptible to additives are more important than that on the nature conformation in determining the transition temperature.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] T.Tomiyama, T.Kai et al.: "The mechanisms by which the transition of (Pro-Pro-Gly)10 changes by addition of alcohols"Polymer Pre.Prints. Vol.52(not known). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kai, T.Tomiyama, et al.: "Is the triple helix of (Pro-Pro-Gly)10 in a single states in solution?"Polymer Pre.Prints. Vol.52(not known). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tomiyama, T.Kai et al.: "Effect of alcohols on the thermal transition of (Pro-Pro-Glt)10"BIOPHYSICS UPPLEMENYT. Vol42. S-19 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kai, T.Tomiyama, et al.: BIOPHYSICS UPPLEMENYT. Vol42. S-48 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kai, T.Tomiyama, et al.: Polymer Pre. Prints. Vol50. E-922 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Nishi, T.Tomiyama, et al.: "Electrostatic Interactions of a Collagen-like Triple-Helical Polypeptides in Solution"Polymer Pre. Prints. Vol.50. E-922 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tomiyama, T.Kai, et.al.: "The mechanisms by which the transition of (Pro-Pro-Gly) 10 changes by addition of alcohols"Polymer Pre.Prints. Vol.52. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kai, T.Tomiyama, et.al.: "Is the triple helix of the (Pro-Pro-Gly) 10 in a single states in solution?"Polymer Pre.Prints. Vol.52. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tomiyama, T.Kai, et.al.: "Effect of alcohols on the thermal transition of (Pro-Pro-Gly) 10"BIOPHYSICALUPPLEMENT. Vol.42. S-12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kai, T.Tomiyama, et.al.: "Is the triple helix of the (Pro-Pro-Gly) 10 in a single states in solution?"BIOPHYSICALUPPLEMENT. Vol.42. S-48 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kai, T.Tomiyama, et.al.: "Effect of Alcohols on the Thermal Transition of collagen Model Peptides in solution"Polymer Pre.Prints. Vol.50. E-922 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Nishi, T.Tomiyama: "Electrostatic Interactions of a Collagen-like Triple-Helical"Polymer Pre.Prints. Vol.50. E-922 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tomiyama, T.kai et al.: "The mechanisms by which the transition of (Pro-Pro-Gly)10 Changes by addition of alcohols"Polymer Pre. Prints. Vol.52(未定). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.kai, T.Tomiyama, et al.: "Is the triple helix of(Pro-Pro-Gly)10 in a single state in solution?"Polymer Pre. Prints. Vol.52(未定). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Tomiyama, T.kai et al.: "Effect of alcohols on the thermal transition of(Pro-Pro-Gly)_<10>"BIOPHYSICS UPPLEMENT. VOL42. S19 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.kai, T.Tomiyama, et al.: "Is the triple helix of(Pro-Pro-Gly)_<10> in a single state in solution?"BIOPHYSICS UPPLEMENT. VOL42. S48 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.kai, T.Tomiyama, et al.: "Effects of Alcohols on the Thermal Transition of Collagen Model Peptides in Solution"Polymer Pre. Prints. Vol.50. E922 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Nishi, T.Tomiyama, et al.: "Electrostatic Interactions of a Collagen-like Triple-Helical Polypeptide in Solution"Polymer Pre. Prints. Vol.50. E922 (2001)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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