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Identification of the gene responsible for host-plant selection in Drosophila sechellia

Research Project

Project/Area Number 13640617
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionTokyo Metropolitan University

Principal Investigator

MATSUO Takashi  Tokyo Metropolitan University, Graduate school of Science, Assistant Professor, 理学研究科, 助手 (70301223)

Co-Investigator(Kenkyū-buntansha) FUYAMA Yohiaki  Tokyo Metropolitan University, Graduate school of Science, Professor, 理学研究科, 教授 (20087133)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsDrosophila sechellia / host-plant selection / 種間変異 / 寄主選好性
Research Abstract

Inter-specific hybrids between Drosophila sechellia and deficiency mutants of D. melanogaster were tested for their preference to caproic acid. Three deficiency strains showed caproic acid preference in this test. Available cytological data, however, did not support the existence of overlapping region among these three deficiencies. Since cytological data are not precise enough, we examined the break points of these deficiencies at molecular level. PCR markers revealed the existence of the overlapping region, and it sizes 80kb. According to the genome project data on D. melanogaster, this region contains 22 predicted genes. Using RT-PCR, we confirmed that all of these genes are expressed in D. sechellia, suggesting that the responsible genetic alteration in D. sechellia affects on the structure of the gene product rather than its expression pattern. Assuming synteny between D. sechellia and D. melanogaster in this region, we screened D. sechellia cosmid library to clone the corresponding genomic fragment, and obtained two independent clones that cover most of the target region. Complete sequence was determined by shotgun sequencing, and was compared with D. melanogaster genomic sequence to identify the responsible gene.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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