| Project/Area Number |
13640654
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | University ofTsukuba |
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Principal Investigator |
ONO Michiyuki University ofTsukuba, Institute of Biological Sciences, Associate Professo, 生物科学系, 助教授 (50201405)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Ardbidopsis / circadian rhythm / day-length / floral induction / flowering / germin-like protein / Pharbitis nil / photoperiodism |
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| Research Abstract |
PnGLP (Pharbitis nil gemin-like protein) is a gene whose transcript is accumulated in leaves and cotyledons of the very sensitive short-day plant, P. nil cv. Violet during the flower-inductive dark-period and encodes a member of the leaf-type subfamily of GLP genes. The functions of GLP proteins are mostly unkown. Northern analysis revealed that the level of PnGLP mRNA showed a circadian rhythm, with maxima around 10 h after the beginning of the dark period, and this oscillation continued in lengthened continuous daikness. Homologues of PnGLP in Aratidcpsis (along-day plait), namely, AtGLP1 and AtGLP2 were isolated and characterized Accumulation of AtGLP1 and AtGLP2 mRNA in leafalso showed circadian rhythms, with maxima around 14 h after the beginning of the light period. These oscillaticns continued mostly under continuous light. The mode of regulation in AtGLP1 and AtGLP2 showed opposite to that of PnGLP, possibly reflecting the differences between short-day plants and long-day plants. To study the mdecularmechanisms ofleaf-type GLP gsne regulation in relati on to photoperiodism, we decided to analyze promoter region ofthese genes using a luciferase gene as a reporter. Because there were no conserved cis-lements among these ptomoteis, we are currently analyzing a series of promoter deletion constructs to identify which sequence motif is essential for circadian rhythmic expression. To deteimine the effects of PnGLP to photoperiodic induction of flowering. we are cumently studying tiansgenic plants expressing PnGLP cDNA. We used short-day tobacco plant (Nicotiona tabacum cv. Maryland Mammoth) as a host plant Tobacco plants that express PnGLP cDNA were likely to form flower earHerwhen they compared to control plant. Tobacco plants that express PnGLP cDNA were likely to from works as a mild stimulator for photoperiodic induction of flowering in short-day plants. Our experiment is currently underway on such hypothesis.
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