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Molecular mechanisms of the initial stages of sexual development in the basidiomycete Coprinus cinereus

Research Project

Project/Area Number 13640666
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KAMADA Takashi  Okayama University, Faculty of Science, Professor, 理学部, 教授 (40033360)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsBasidiomycetes / Coprinus cinereus / Sexual development / Matins-tune genes / Dikaryon / Nuclear migration / きのこ / クランプ結合 / 高配型遺伝子 / ニ核菌糸
Research Abstract

Molecular mechanisms of the initial stages of sexual development in the basidiomycete Coprinus cinereus were studied with the following results.
1. We found that progeny isolated from a fruiting body collected in the field exhibit a distinctive mycelial development in common A matings. Genetic analysis suggested that the common A heterokaryotic phenotype is brought about by a nuclear factor(s) other than the mating type genes.
2. We identified the whole structure of the num1 gene, which is involved in nuclear migration for dikaryosis, a major B-regulated developmental process.
3. We screened a cDNA library for clones that encode proteins interacting with Num1, which identified the nip1 gene.
4. We analyzed the promoter region of the clp1 gene and identified a 10-bp sequence (5'-GATGCAAACA-3') located 155-146 upstream of the transcriptional start site, which is involved in the transcriptional control of clp1 In the future, we plan to examine whether the heterodimer of the A mating-type gene products (HD1 and HD2), which activates clp1 expression, binds the 10-bp sequence.
5. We found that Clp1 and Pcc1, both of which are working in the A-regulated developmental pathway, are interacting with each other, as examined by yeast two-hybrid assays. Furthermore, we identified that the C-terminal region of Clp1 and the N-terminal region of Pcc1 are involved in the interaction in yeast.
6. To elucidate downstream events of pcc1, we performed a subtraction experiment using the pcc1-4 mutant strain (5337#4), in which the A-regulated developmental pathway is constitutively activated, and its parental wild-type homokaryon (5337), and selected 64 cDNAs from genes possibly up-regulated in strain 5337#4. In the future, we plan to confirm differential transcription between strains 5335 and 5337#4 for each cDNA by northern analysis and then analyze the structure and function of genes expressed differentially between 5337 and 5337#4.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(14 results)

All Other

All Publications

  • [Publications] Hajime Muraguchi: "A distinctive phenotype in the common-A heterokaryon of Coprinus cinereus"Mycoscience. 43. 77-79 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takashi Kamada: "Molecular genetics of sexual development in the mushroom Coprinus cinereus"BioEssays. 24. 449-459 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Rika Makino: "Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus"Current Genetics. 45. 149-156 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 鎌田 堯(分担執筆): "キノコとカビの基礎科学とバイオ技術(宍戸和夫編著)"アイピーシー. 564 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Muraguchi, H., T.Kamada: "A distinctive phenotype in the common A heterokaryon of Coprinus cinereus"Mycoscience. 43. 77-79 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takashi Kamada: "Molecular genetics of sexual development in the mushroom Coprinus cinereus"BioEssays. 24. 449-459 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Makino, R., T.Kamada: "Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus"Current Genetics. 45. 149-156 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takashi Kamada (partial contribution): "Basic Science and Biotechnology of Mushrooms and Molds"IPC. 564 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Rika Makino: "Isolation and characterization of mutations that affect nuclear migration for dikaryosis in Coprinus cinereus"Current Genetics. 45. 149-156 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hajime Muraguchi: "A distinctive phenotype in the common-A heterokaryon of Coprinus cinereus"Mycoscience. 43. 77-79 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takashi Kamada: "Molecular genetics of sexual development in Coprinus cinereus"BioEssays. 24. 449-459 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 鎌田 堯(分担執筆): "キノコとカビの基礎科学とバイオ技術"アイピーシー. 564 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hajime Muraguchi: "A distinctive phenotype in the common-A heterokaryon of Coprinus cinereus"Mycoscience. 43(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Kamada: "Molecular genetics of sexual development in Coprinus cinereus"BioEssays. 24(in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-03-31   Modified: 2016-04-21  

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