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Neural mechanisms conroling adult diapause in insects

Research Project

Project/Area Number 13640686
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionOsaka City University

Principal Investigator

NUMATA Hideharu  Osaka City University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (70172749)

Co-Investigator(Kenkyū-buntansha) SHIGA Sakiko  Osaka City University, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (90254383)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsadult diapause / neurosecretory cells / Protophormia terraenovae / pars intecerebralis / pars lateralis / corpus allatum / juvenile hormone / ハエ / FMRFアミド / 休眠
Research Abstract

The present study was performed with an aim to investigate neural mechanisms underlying reproductive diapause in the blow fly, Protophormia terraenovae. In vitro synthetic activities of juvenile hormones (JH) were examined using a radiochemical assay in diapause females and reproductive females. JH synthetic activities increased in females reared under diapause-averting conditions and remained low in previtellogenic females reared under diapause-inducing conditions. A decrease in the JH production rate is a cause of diapause induction. JH synthetic rate after removal of the pars intercerebralis (PI) and pars lateralis (PL) neurons suggested that these neurons do not directly mediate changes in the JH production rate, but regulate ovarian development cooperatively with some unknown allatostatic and allatotropic factors. Morphological and electrical properties of PI and PL neurons were examined by intracellular recording and staining. Three types of PI and two types of PL neurons were identified. Among these neurons, one PI neuron showed significant differences in spike durations and one PL neuron in spike heights between diapause-inducing and diapause-averting conditions. FMRFamide, β-pigment-dispersing hormone, corazonin and cholecystokinin-like peptides were immunocytochemically revealed in the PI or PL neurons. FMRFamide and cerebral lobes weakly suppressed JH biosynthetic rates in vitro. The present study morphologically and electrophysiologically characterized PI and PL neurons, and showed candidates for factors controlling JH synthetic rates in neural mechanisms for reproductive diapause in P.terraenovae.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Shiga, S: "Anatomy and functions of brain neurosecretory cells in Diptera"Microscopy Research and Technique. 62. 114-131 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiga, S., Hamanaka, Y., Tatsu, Y., Okuda, T., Numata, H.: "Juvenile hormone biosynthesis in diapause and nondiapause females of the adult blow fly Protophormia terraenovae."Zoological Science. 20. 1199-1206 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamanaka, Y., Numata, H., Shiga, S.: "Morphology and electrophysiological properties of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae."Cell and Tissue Research. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiga, S.: "Anatomy and functions of brain neurosecretory cells in Diptera"Microscopy Research and Technique. 62. 114-131 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiga, S., Hamanaka, Y., Tatsu, Y., Okuda, T., Numata, H.: "Juvenile hormone biosynthesis in diapause and nondiapause females of the adult blow fly Protophormia terraenovae"Zoological Science. 20. 1199-1206 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamanaka, Y., Numata, H., Shiga, S.: "Morphology and electrophysiological properties of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae"Cell and Tissue Research. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiga S., Hamanaka Y., Tatsu Y., Okuda T., Numata H: "Juvenile hormone biosynthesis in diapause and nondiapause females of the adult blow fly Protophormia terraenovae."Zoological Science. 20. 1199-1206 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiga S.: "Anatomy and functions of brain neurosecretory cells in Diptera."Microscopy Research and Technique. 62. 114-131 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 沼田英治: "私のおすすめ実験生物22 ルリキンバエProtophormia terraenovae"細胞工学. 22. 1110-1111 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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