|Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
In the present study, we revealed that an analysis of DMA sequence of plastid tmK gene 5' intron region is often very effective in order to construct the phylogenetic tree that provides high resolution of relationships among species / infraspecific taxa Thus, we conducted not a single gene analysis of matK but a combined analysis of tmK 5' intron and matK to construct the exact phylogenetic tree of Heloniopsis, which is one of the morphologically primitive genera among the monocotyledons. And, using this phylogenetic tree, we studied this genus from phylogenetical, phylogeographical and taxonomical points of view. As the results, we revealed that the Korean Heloniopsis, which has been considered to be the same with H. orientals in Japan, consists of two new species. And we named them H. tubiflora and H. koreana, respectively. We revealed also that the plants in Kanto Province, which have been assigned to H. orientals var. flavida (=H. breviscapa var flavida), are H. breviscapa var, breviscapa, and H. breviscapa var. breviscapa is disjunctly distributed in Kanto Province and Chugoku-Shikoku-Kyushu Provinces. Consequently, we established a new classification of Heloniopsis, where seven species and one variety are recognized.
In the present study, besides the molecular analyses, we analyzed morphological characters of Polygonatum and Hosta (Liliaceae sensu lato) and Dioscorea (Dioscoreaoeae), which are all regarded as morphologically primitive monocotyledons. And, we proposed several new species, new varieties and new combinations. Further, the several Polygonatum taxa that have been considered as independent species turned out to be hybrids between different species. These results are included in Liliaceae and Dioscoreaceae chapters of Flora of Japan IVb (Kodansha, Tokyo).