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Biomedical measurement using confocal-differential-interference contrast microscope

Research Project

Project/Area Number 13650035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied optics/Quantum optical engineering
Research InstitutionUniversity of Tsukuba

Principal Investigator

ITOH Masahide  University of Tsukuba, Graduate School of Pure and Applied Sciences, Associate Professor, 大学院・数理物質科学研究科, 助教授 (30150874)

Co-Investigator(Kenkyū-buntansha) UCHIDA Akiko  Foundation for Advancement of International Science, reserture, 専任研究員 (30241813)
YATAGAI Toyohiko  University of Tsukuba, Graduate School of Pure and Applied Sciences, Professor, 大学院・数理物質科学研究科, 教授 (90087445)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
KeywordsSpectral analysis / confocal microscope / differential interference / phase shift method / biomedical measurement / Nomalski / 光散乱 / 微分干渉顕微鏡 / スペクトル / 共焦点光学系 / 皮膚 / 光散乱体 / DIC / 分光 / 顕微法
Research Abstract

The Nomalski type differential interference contrast microscope is widely used in the field of the semiconductor industry and the biomedicine. On the other hand, a laser confocal scanning microscope is excellent in resolution in the vertical direction. First of all, we researched to take quantitative shape data from the differential interference microscope image. This is a method to extract quantitative shape data of the sample from two or more images with a different phase delay of the differential interference microscope. The influence was able to be decreased when a scatterer exist near the object by using this technique. The structure of the tissue of the human skin was able to be observed as an application to the living body measurement. Moreover, as the two or more light source with different wavelength was used, measurement range was able to be expanded though there was a problem that the uncertainty remained in the data when there was an optical path difference more than wavele … More ngth in a conventional interference microscope. In the next, the problem of the confocal microscope was analyzed, and the application of the technique of the phase modulation type differential interference microscope was researched. If the weak phase object is observed by the confocal microscope the discernment or the obscure lines is observed in the edge, and it becomes false-detection of edge shape. The interference of the diffraction light from the edge seems to cause in this effect. The image formation characteristic of the confocal microscope is analyzed by the product of the transfer function of the effect of optical sectioning (I-z characteristic) and the image formation system. Therefore, it has been understood that the peak of image strength is caused at a prescribed spatial frequency in case of the defocus, and the edge emphasis happens as a result. It has been understood that the false-detection in the confocal microscope is able to correct by applying the differentiation interference method which is the phase metrology. As a result, we have a new indicator to develop a microscope to combine confocal and differential microscope to measure a biomedical sample. Less

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(25 results)

All 2004 2003 2002 2001 Other

All Journal Article Publications

  • [Journal Article] Holographic Recording on Azo-benzene Functionalized Polymer Film2004

    • Author(s)
      M.Itoh, K.Harada, S.Kamemaru, T.Yatagai
    • Journal Title

      Japanese Journal of Applied Physics 43・7B

      Pages: 4968-4968

    • NAID

      10013432476

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Computer simulation of photoinduced mass transport on azobenzene polymer films by particle method2004

    • Author(s)
      D.Barada, M.Itoh, T.Yatagai
    • Journal Title

      Journal of Applied Physics 96・8

      Pages: 4204-4204

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Full-color computer-generated holograms using 3-D Fourier spectra2004

    • Author(s)
      Y.Sando, M.Itoh, T.Yatagai
    • Journal Title

      Optics Express 12

      Pages: 6246-6246

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Holographic Recording on Azo-benzene Functionalized Polymer Film2004

    • Author(s)
      M.Itoh, K.Harada, S.Kamemaru, T.Yatagai
    • Journal Title

      Japanese Journal of Applied Physics, 43, 7B

      Pages: 4968-4968

    • NAID

      10013432476

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Computer simulation of photoinduced mass transport on azobenzene polymer films by particle method2004

    • Author(s)
      D.Barada, M.Itoh, T.Yatatgai
    • Journal Title

      Journal of Applied Physics 96, 8

      Pages: 4204-4204

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Full-color computer-generated holograms using 3-D Fourier spectra2004

    • Author(s)
      Y.Sando, M.Itoh, T.Yatagai
    • Journal Title

      Optics Express 12, 2

      Pages: 6246-6246

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Computer simulation of photoinduced mass transport on azobenzene polymer films by particle method2004

    • Author(s)
      D.Barada, M.Itoh, T.Yatatgai
    • Journal Title

      Journal of Applied Physics 96・8

      Pages: 4204-4204

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Color computer-generated holograms from projection images2004

    • Author(s)
      Y.Sando, M.Itoh, T.Yatagai
    • Journal Title

      Optics Express 12

      Pages: 2484-2484

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Polarization-sensitive complex Fourier domain optical coherence tomography for Jones matrix imaging of biological samples2004

    • Author(s)
      Y.Yasuno, S.Makita, T.Endo, M.Itoh, T.Yatagai, M.Takahashi, C.Katada, M.Mutoh
    • Journal Title

      Applied Physics Letters 85

      Pages: 3023-3023

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A subfringe integration method for multiple-beam Fizeau fringe analysis2003

    • Author(s)
      El-Morsy, M.A., Harada, K., Itoh, M., Yatagai, T.
    • Journal Title

      Optics & Laser Technology 35

      Pages: 223-223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A subfringe integration method for multiple-beam Fizeau fringe analysis2003

    • Author(s)
      EI-Morsy, M.A., Harada, K., Itoh, M., Yatagai, T.
    • Journal Title

      Optics & Laser Technology 35

      Pages: 223-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spatial light manipulation devices using nonlinear polymeric materials2002

    • Author(s)
      Harada, K., Munakata, K., Itoh, M., Umegaki, S., Yatagai, T.
    • Journal Title

      Optics and Quantum Electronics 34

      Pages: 1183-1183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of a new technique for measuring a blotch2001

    • Author(s)
      Masuda, Y., Takahashi, M., Sakamoto, T., Shimada, M., Itoh, M., Yatagai, T.
    • Journal Title

      Journal of Society of Cosmetic Chemistry Japan 35

      Pages: 325-325

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] M.A.El-Morsy et al.: "A subfringe integration method for multiple beam Fizeau fringe analysis"opt & Laser Technol.. 3・5. 223 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S Makita et al.: "Polarization sensitive spectral interferometric optical coherence fomography for human skin imaging"Opt.Rev. 10・5. 366 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Yasno et al.: "Birefrengence imaging of human skin by polarization-sensitive spectral interferometvic OCT"Opt.Lett.. 27・20. 1803 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Harada et al.: "Spatial bgho modelation device using nonlineau polymeric materials"Opt.Quant.Electro.. 34・12. 1183 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.YASONO et al.: "Aberration measurement from confocal axial intensity response using neural network"Optics Express. 10・25. 1451 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.A.El-Morsy et al.: "A subfringe integration method for multiple-beam Fizeau fringe analysis"Opt & Laser Tech.. 35. 223 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Haradu et al.: "Spatial light manipulation devices using polymeric materials"Otp. and Quant Electr.. 34・12. 1183 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.YASUNO et al.: "Brrefringe imaging of human skin by polarization-sensitive spectral interferometric optical coherence tomography"Opt. Lett.. 27・20. 1803 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Shimada, Y.Yamada, M.Itoh, T.Yatagai: "Melanin and blood concentration in a human skin model studied by multiple regression analysis"Phys. Med. Biol.. 46巻. 2397-2406 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Munakata et.al.: "A new holographic recording material and its diffraction efficiency increase effect"Opt. Commun.. 191巻. 15-19 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 多田勇二 他: "新しいシミ計測法の開発"日本化粧品技術者会誌. 35巻4号. 325-332 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 伊藤雅英 ほか: "位相変調型微分干渉顕微鏡"光学. 30巻・9号. 605-612 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-03-31   Modified: 2016-04-21  

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