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Characterization of the enzyme system catalyzing Anammox

Research Project

Project/Area Number 13650861
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionSojo University

Principal Investigator

FUJII Takao  Sojo university, Department of Engineering, Professor, 工学部, 教授 (80165331)

Co-Investigator(Kenkyū-buntansha) SUGINO Hiroyuki  Sojo university, Department of Engineering, 工学部, Lecturer (90258434)
FURUKAWA Kenji  Kumamoto university, Department of Engineering, Professor, 工学部, 教授 (60029296)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsanaerobic ammonium oxidation / Anammox / Planctomycete / denitrification / treatment of nitrite and ammonium / Planctomycete / FISH
Research Abstract

The enrichment and characterization of anaerobic ammonium-oxidizing (Anammox) biofilm cultures for the treatment of groundwater in Kumamoto, are ongoing in our laboratories. To investigate the bacterial composition of the mature biofilm community, 16S rDNA sequences were amplified by PCR and comparative analyses using DNA databases were conducted. Only one sequence had a notable similarity (92.2%) to that of the first discovered anaerobic ammonium-oxidizing planctomycete, Candidatus Brocadia anammoxidans, The newly discovered strain (designated KSU-1) was dominant among detectable members of the biofilm community. By fluorescence imaging, KSU-1 was shown to form spherical clusters wrapped in a thin layer of Zoogloea sp.
We attempted to purify the hydroxylamine oxidoreductase(hao) seemed as a critical enzyme in Anammox. The hao was extracted from the enriched Anammox biofilm, purified with DEAE-cellulose Superdex gel filtration column chromatography, and dialyzed with a cellulose membrane passing less than 50 kDa molecules. The purified hao was homogeneous by SDS-PAGE, exhibiting a 110 kDa band smaller than that of hao purified from Candidatus Brocadia anammoxidans. Although the hao showed similar spectra to that from Nitrosomonas europeae, hydrazine gave lager spectral change of the hao than hydroxylamine, suggesting it has higher affinity to hydrazine than hydroxylamine.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] U.Imajo, H.Ishida, T.Fujii, H.Sugino, J.Rouse, K.Furukawa: "Detection of anammox activity from activated sludges"Asian water qual 2001, Asia-pacific regional conference procceding. (CD-ROM). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Furukawa, J.Rouse, U.Imajo, H.Sugino, T.Fujii: "Establishment of an aerobic ammonium-oxidizing culture in continuous treatment with non-woven biomass carrier"Proceeding of Water environment federation, 74th annual conference and exposition. (CD-ROM). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Fujii, H.Sugino, J.D.Rouse, K.Furukawa: "Characterization of the microbial community in an anaerobic ammonium-oxidizing biofilm cultured on a nonwoven biomass carrier"Journal of Bioscience and Bioengineering. 94. 412-418 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] U. Imajo: "Detection of anammox activity from activated sludges"Asian water qual 2001, Asia-pacific regional conference proceeding, CD-ROM. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Furukawa: "Establishment of an aerobic ammonium-oxidizing culture in continuous treatment with non-woven biomass carrier"Proceeding of Water environment federation, 74th annual conference and exposition, CD-ROM. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Fujii: "Characterization of the microbial community in an anaerobic ammonium-oxidizing biofilm cultured on a nonwoven biomass carrier"Journal of Bioscience and Bioengineering. 94. 412-418 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] U.Imajo, H.Ishida, T.Fujii, H.Sugino, J.Rouse, K.Furukawa: "Detection of anammox activity from activated sludges"Asian water qual 2001, Asia-pacific regional conference proceeding. (CD-ROM). (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Furukawa, J.Rouse, U.Imajo, H.Sugino, T.Fujii: "Establishment of an aerobic ammonium-oxidizing culture in continuous treatment with non-woven biomass carrier"Proceeding of Water environment federation, 74th annual conference and exposition. (CD-ROM). (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Fujii, H.Sugino, J.D.Rouse, K.Furukawa: "Characterization of the microbial community in an anaerobic ammonium-oxidizing biofilm cultured on a nonwoven biomass carrier"Journal of Bioscience and Bioengineering. 94. 412-418 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] U.Irnajo, H.Ishida, T.Fujii, H.Sugino, J.Rouse, K.Furukawa: "Detection of anammox activity from activated sludges"Asian water qual 2001, Asia-pacific regional conference procceding. (CD-ROM). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Furukawa, J.Rouse, U.Imajo, H.Sugino, T.Fujii: "Establishment of an aerobic ammonium-oxidizing culture in continuous treatment with non-woven biomass carrier"Proceeding of Water environment federation, 74th annual conference and exposition. (CD-ROM). (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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