| Project/Area Number |
13660001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KISHIMA Yuji Hokkaido Univ., Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (60192556)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Antirrhinum majus / transposon / Tam3 / low temperature stress / DNA methylation / gel shift assay / 5-aza-cytidine / トランスポゾンTam3 / 低温依存性転移 / DNAのメチル化 / 重亜硫酸ナトリウム処理実験 / タンパク質のDNA結合実験 / 転移酵素タンパク質 |
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| Research Abstract |
The transposition frequency of Tam3 in Antirrhinum, unlike that of most other cut-and-paste type transposons, is tightly controlled by temperature : Tam3 transposes rarely at 25℃, but much more frequently at 15℃. Here we studied the mechanism of the low-temperature-dependent transposition (LTDT) of Tam3. Our results strongly suggest that LTDT is not likely to be due to either transcriptional regulation or posttranscriptional regulation of the Tam3 Tpase gene. We found that temperature shift induced a remarkable change of the methylation state unique to Tam3 sequences in the genome : higher temperature resulted in hypermethylation, while lower temperature resulted in reduced methylation. The methylation state was reversible within a single generation in response to a temperature shift. Although our data demonstrate a close link between LTDT and the methylation of Tam3, they also suggest that secondary factor(s) other than DNA methylation is involved in repression of Tam3 transposition.
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