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Genetic analysis of Vrn genes and establishment of their DNA markers in wheat by using Single Nucleotide Polymorphisms (SNPs)

Research Project

Project/Area Number 13660009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionOkayama University

Principal Investigator

KATO Kenji  Okayama University, Fac.of Agriculture, Associate Professor, 農学部, 助教授 (40161096)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsWheat / Vernalization / DNA marker / SSR / dCAPS / AFLP / マイクロサテライト / 日長反応性
Research Abstract

Molecular genetic study has been conducted in the present study, and succeeded to establish molecular markers for the selection of four genes for vernalization response, Vrn-A1,Vrn2,Vrn-D1,Vrn4. The detail is given below for each gene.
Vrn-A1 ; We could confirm the linkage (12.8cM) with RFLP locus Xwg908,at which CAPS marker was established.
Vrn2 ; Linkage with a dCAPS marker of Xwg644-5B and SSR markers (Xgwm408 and Xgwm604) was detected, and four loci could be mapped as follows ; Vrn2-1.6cM-Xwg644-2.5cM-Xgwm408-13.8cM-Xgwm604. As a result, Vrn2 was located on chromosome 5B and we proposed to rename Vrn2 as Vrn-B1. Xgwm408 can be utilized as a practical selection marker of Vrn-B1, since it is closely linked with Vrn-B1 and clear polymorphism can be detected among the Japanese wheat cultivars and the foreign cultivars carrying Vrn-B1.
Vrn-D1 ; Vrn-D1 is the most important gene for wheat breeding in Japan, since most of the wheat cultivars with spring growth habit carry this gene. Two SSR … More markers (Xgwm212 and Xgwm292) proved to link with Vrn-D1, at a distance of 4.6cM and 7.4cM, respectively. Although Xgwm292 was rather distantly located, it was highly polymorphic even in the Japanese early maturing cultivars. Therefore, this SSR marker can be utilized as a practical selection marker for the breeding of early maturing cultivars with spring or winter growth habit.
Vrn4 ; Several SSR markers, such as Xgwm190,Xgdm3 and Xgwm292 located on 5D chromosome, were analyzed and close linkage (4.5cM) was detected among Vrn4 and Xgdm3. Independence between Vrn4 and Xgwm292, which is closely linked to Vrn-D1, was also confirmed. It was therefore concluded that Vrn4 is located near the centromere on the long arm of 5D chromosome and independent of Vrn-D1. Vrn4 proved to be a new class of vernalization response gene which was different from Vrn-1 orthologue gene, and we proposed to designate as Vrn-D5.
Vernalization response genes causing the difference in the degree of winter growth habit were analyzed in DH populations derived from a cross between two winter type cultivars, Winter-type Abukumawase and Chihokukomugi, by using AFLP markers. As a result, three QTL was detected and one QTL was located on the short arm of 7D chromosome. The effect of Vrn-D1 was also suggested. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All 2003 2002 2001 Other

All Journal Article (9 results) Publications (10 results)

  • [Journal Article] Genetic analysis of two genes for vernalization response, the former Vrn2 and Vrn4, by using PCR based molecular markers.2003

    • Author(s)
      K.Kato, M.Yamashita, K.Ishimoto, H.Yoshino, M.Fujita
    • Journal Title

      Proc. 10th Inter. Wheat Genet. Symp. 3

      Pages: 971-973

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] コムギの春播性遺伝子Vrn-A1, Vrn-B1, 及びVrn-D1に関する連鎖PCRマーカー2003

    • Author(s)
      加藤鎌司, 石本慶一郎, 山下美穂, 藤田雅也
    • Journal Title

      育種学研究 5(別冊1)

      Pages: 146-146

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] コムギの5D染色体長腕に座乗する第2の春播性遺伝子Vrn4のマッピング2003

    • Author(s)
      加藤鎌司, 山下美穂, 石本慶一郎, 吉野煕道, 藤田雅也
    • Journal Title

      育種学研究 5(別冊2)

      Pages: 95-95

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Genetic analysis of two genes for vernalization response, the former Vrn2 and Vrn4, by using PCR based molecular markers.2003

    • Author(s)
      K.Kato, M.Yamashita, K.Ishimoto, H.Yoshino, M.Fujita
    • Journal Title

      Proc.10th Inter.Wheat Genet.Symp. Vol.3

      Pages: 971-973

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivumL.)2002

    • Author(s)
      K.Iwaki, J.Nishida, T.Yanagisawa, H.Yoshida, K.Kato
    • Journal Title

      Theoretical & Applied Genetics 104

      Pages: 571-576

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.)2002

    • Author(s)
      K.Iwaki, J.Nishida, T.Yanagisawa, H.Yoshida, K.Kato
    • Journal Title

      Theor.Appl.Genet. 104

      Pages: 571-576

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The possible candidate of Vrn-B1 in wheat, as revealed by monosomic analysis of Vrn gene carried by Triple Dirk(B), the former Vrn2.2001

    • Author(s)
      K.Iwaki, K.Nakagawa, K.Kato
    • Journal Title

      Wheat Information Service 92

      Pages: 9-11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Adaptation and ecological differentiation in wheat with special reference to geographical variation of growth habit and Vrn genotype.2001

    • Author(s)
      K.Iwaki, S.Haruna, T.Niwa, K.Kato
    • Journal Title

      Plant Breed. 120

      Pages: 107-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The Possible candidate of Vrn-B1 in wheat, as revealed by monosomic analysis of Vrn gene carried by Triple Dirk(B), The former Vrn2.2001

    • Author(s)
      K.Iwaki, K.Nakagawa, K.Kato
    • Journal Title

      Wheat Inf.Serv. 92

      Pages: 9-11

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kato, M.Yamashita, K.Ishimoto, H.Yoshino, M.Fujita: "Genetic analysis of two genes for vernalization response, the former Vrn2 and Vrn4, by using PCR based molecular markers"Proc.10th Inter.Wheat Genet.Symp.. 3. 971-973 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 加藤鎌司, 石本慶一郎, 山下美穂, 藤田雅也: "コムギの春播性遺伝子Vrn-A1,Vrn-B1,及びVrn-D1に関する連鎖PCRマーカー"育種学研究. 5(別冊1). 146 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 加藤鎌司, 山下美穂, 石本慶一郎, 吉野煕道, 藤田雅也: "コムギの5D染色体長腕に座乗する第2の春播性遺伝子Vrn4のマッピング"育種学研究. 5(別冊2). 95 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Iwaki, J.Nishida, T.Yanagisawa, H.Yoshida, K.Kato: "Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.)"Theoretical & Applied Genetics. 104. 571-576 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 加藤鎌司, 小原義規, 後藤志乃, 柳沢貴司, 吉田久, 藤田雅也: "秋播型コムギDH集団を用いた出穂特性の遺伝解析"育種学研究. 4(別冊1). 197 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 加藤鎌司, 後藤志乃, 柳沢貴司, 藤田雅也: "AFLPマーカーを用いコムギ出穂特性の遺伝解析"育種学研究. 4(別冊2). 283 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 加藤鎌司, 石本慶一郎, 山下美穂, 藤田雅也: "コムギの春播性遺伝子Vrn-A1,Vrn-B1,及びVrn-D1に関する連鎖PCRマーカー"育種学研究. 5(別冊1)(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Iwaki, K.Nakagawa, K.Kato: "The possible candidate of Vrn-B1 in wheat, as revealed by monosomic analysis of Vrn gene carried by Triple Dirk(B), the former Vrn2"Wheat Information Service. 92. 9-11 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Iwaki, J.Nishida, T.Yanagisawa, H.Yoshida, K.Kato: "Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.)"Theoretical & Applied Genetics. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤鎌司, 小原義規, 後藤志乃, 柳沢貴司, 吉田久, 藤田雅也: "秋播型コムギDH集団を用いた出穂特性の遺伝解析"育種学研究. 4(別冊1)(印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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