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Mechanism of suppression of host-plant defense PR1a gene expression by the plant tumor-inducing 6b gene of Agrobacterium tumefaciens

Research Project

Project/Area Number 13660055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionAkita Prefectural University

Principal Investigator

WABIKO Hiroetsu  Akita Prefectural University, Faculty of Bioresource Sciences, Professor, 生物資源科学部, 教授 (10191842)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsPR1a / Agrobacterium / AK-6b gene / Salicylic acid / auxin / T7.Tag His.Tag / PRI / 6b遺伝子 / デキサメタゾン / His-tag / 植物ホルモン / タバコ / PR1 / フェノール化合物 / サイトカイニン
Research Abstract

Expression of plant defense genes, termed Pathogenesis Related (PR) genes, is turned on upon infection of pathogens to plants. A soil bacterium Agrobacterium tumefaciens induces crown gall tumors on a number of plants. We have shown that the tumor-inducing 6b (AK-6b) gene obtained from a strain AKE10 enhanced the accumulation of several phenylpropanoids, which also possess anti-pathogenic activity. Since regulatory factor(s) responsible for PR gene overlaps with the biosynthesis of phenylpropanoid, we analyzed the effect of AK-6b gene on PR1a gene expression. We generated tobacco plants transgenic for the AK-6b gene under the control of the promoters of its own, CaMV35S, and dexamethazone inducible ones. Tissues were grown in the presence or absence of plant hormones, mRNA was extracted, and PR1a gene expression was analyzed by Northern hybridization. There was no obvious relationship between PR1a transcript levels and AK-6b transcript levels. However, when timorous tissues were cultivated on hormone-free medium and transferred to auxin-containing medium and followed time course of PR1a transcript accumulation, there was a clear difference. It was found that PR1a mRNA levels of transgenic tissues were reduced compared to those of wild type tobacco tissues until 6 days post transfer. At day 6, PR1a transcript of both tissues was comparable. We concluded, therefore, that both the AK-6b gene expression and presence of auxin were responsible for reduction of PR1a mRNA accumulation, and that the reduction was transient. Exogenous application of salicylic acid induced PR1a transcripts of both transgenic and wild type tissues, demonstrating transgenic tissues possess a normal potential to induce PR1a gene expression. We also purified modified AK-6b protein tagged with T7 and His, to near homogeneity by using affinity chromatography.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All 2004 2002 2001 2000 1996 1988 Other

All Journal Article (12 results) Publications (5 results)

  • [Journal Article] Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco2004

    • Author(s)
      Galis I, Kakiuchi Y, Simek P, Wabiko H
    • Journal Title

      Phytochemistry 65

      Pages: 169-179

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] アグロバクテリウムハンター-遺伝子導入ベクターとしての普遍的な性質と新規株の特性-2004

    • Author(s)
      我彦広悦
    • Journal Title

      化学と生物 42

      Pages: 29-34

    • NAID

      10012135498

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco.2004

    • Author(s)
      Galis, I., Kakiuchi, Y., Simek, P., Wabiko, H.
    • Journal Title

      Phytochemistry 65

      Pages: 169-179

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Resistance of transgenic tobacco seedlings expressing the Agrobacterium tumefaciens C58-6b gene, to growth-inhibitory levels of cytokinin is associated with elevated IAA levels and activation of phenylpropanoid metabolism2002

    • Author(s)
      Galis I, Simek P, Van Onckelen HA, Kakiuchi Y, Wabiko H
    • Journal Title

      Plant Cell Physiol 43

      Pages: 939-950

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The protein encoded by oncogene 6b from Agrobacterium tumefaciens interacts with a nuclear protein of tobacco2002

    • Author(s)
      Kitakura S, Fujita T, Ueno Y, Terakura S, Wabiko H, Machida Y
    • Journal Title

      Plant Cell 14

      Pages: 451-463

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The phenylpropanoid pathway and plant defence-a genomics perspective.2002

    • Author(s)
      Dixon, R.A., Achnine, L.a., Kota, P., Liu, C., Reddy, M.S., Wang, L.
    • Journal Title

      Mol.Plant Pathol. 3

      Pages: 371-390

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Resistance of transgenic tobacco seedlings expressing the Agrobacterium tumefaciens C58-6b gene, to growth-inhibitory levels of cytokinin is associated with elevated IAA levels and activation of phenylpropanoid metabolism.2002

    • Author(s)
      Galis, I., Simek, P., Van Onckelen, H.A., Kakiuchi, Y., Wabiko, H.
    • Journal Title

      Plant Cell Physiol 43

      Pages: 939-950

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The protein encoded by oncogene 6b from Agrobacterium tumefaciens interacts with a nuclear protein of tobacco.2002

    • Author(s)
      Kitakura, S., Fujita, T., Ueno, Y., Terakura, S., Wabik, H., Machida, Y.
    • Journal Title

      Plant Cell 14

      Pages: 451-463

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] アグロバクテリウムの腫瘍形成遺伝子による植物細胞の脱分化,再生機構2001

    • Author(s)
      我彦広悦, Galis Ivan, 垣内康孝
    • Journal Title

      植物の生長調節 36

      Pages: 24-34

    • NAID

      110001846732

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Expression of a pathogen-induced gene can be mimicked by auxin insensitivity.2000

    • Author(s)
      Mayda, E., Marques C., Conejero, V., Vera P.
    • Journal Title

      Mol.Plant-Mirobe Interact. 13

      Pages: 23-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Exogenous phytohormone-independent growth and regeneration of tobacco plants transgenic for the 6b gene of Agrobacterium tumefaciens AKE10.1996

    • Author(s)
      Wabiko, H., Minemura, M.
    • Journal Title

      Plant Physiol 112

      Pages: 939-951

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The Agrobacterium tumefaciens T-DNA gene 6b is an onc gene.1988

    • Author(s)
      Hooykaas, P.J.J., den Dulk-Ras, H., Schilperoort, R.A.
    • Journal Title

      Plant Mol Biol 11

      Pages: 791-794

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Galis I, Kakiuchi Y, Simek P, Wabiko H: "Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco."Phytochemistry. 65. 169-179 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kitakura S, Fujita T, Ueno Y, Terakura S, Wabiko H, Machida Y: "The protein encoded by oncogene 6b from Agrobacterium tumefaciens interacts with a nuclear protein of tobacco"The Plant Cell. 14. 451-463 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Galis I, Simek P, Onckelen HA, Kakiuchi Y, Wabiko H: "Resistance of transgenic tobacco seedlings expressing the Agrobacterium tumefaciens C58-6b gene, to growth-inhibitory levels of cytokinin is associated with elevated IAA levels and activation of phenyipropanoid metabolism"Plant and Cell Physiology. 43. 939-950 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] J.Kaneyoshi, H.Wabiko, S.Kobayashi, T.Tsuchiya: "Agrobacterium tumefaciens AKE10-mediated transformation of an Asian pea pear, Pyrus betulaefolia Bunge : host specificity of bacterial strains"Plant Cell Reports. 20. 622-628 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 我彦広悦, Galis Ivan, 垣内康孝: "アグロバクテリウムの腫瘍形成遺伝子による植物細胞の脱分化、再生機構"植物の生長調節. 36. 24-34 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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