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Isolation and characterization of aluminum stress resistant genes using activati on tagging lines of Arabidopsis thaliana

Research Project

Project/Area Number 13660066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

EZAKI Bunichi  Okayama University, Research Institute for Bioresources, Assistant Professor, 資源生物科学研究所, 助手 (90243500)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Hideaki  Okayama University, Research Institute for Bioresources, Professor, 資源生物科学研究所, 教授 (80026418)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsResistant mechanism for Al stress / Activation tagging line / Al stress resistant gene / Root hair / Al toxicity / Arabidopsis thaliana / Root hair mutant / Transgenic plant / 形質転換体植物 / ストレス耐性 / Aluminum(アルミニウム)ストレス / 酸化ストレス / 耐性遺伝子 / 遺伝子操作 / Arabidopsis(シロイヌナズナ)
Research Abstract

To isolate new Al resistant genes, we performed a screening of resistant plants from a pool of Arabidopsis enhancer tagging lines. From approximately 16,000 lines, 20 clones were isolated as Al resistant candidates. Southern hybridization indicated that one line, #355-2, only carries single copy of the enhancer tag in chromosome DNA. A TAIL-PCR was performed for #355-2, and the result indicated that the tag is inserted in chromosome I (very closed to up-stream region of the F9E10.5 gene). RT-PCR was also carried out and we found that a higher gene expression of F9E10.5 and F9E10.6 was specifically detected in #355-2 line (approximately 8-and 7-folds, respectively) than control line (Col-0). DNA database analysts indicated that F9E10.6 encodes a DNA glycosylase, but the function of F9E10.5 is unknown.
To characterize the A resistant mechanisms in #355-2 line, morphological observation of Col-0 and #355-2 was performed and we could see a clear difference in root-hair region. The length of root-hair of #355-2 was much shorter (approximately 1/3) than that of Col-o. Al treatment (300-500 μM, 2d) for these two lines indicated that their root-hairs were completely damaged by Al toxicity. Moreover, accumulation of Al in root region of #355-2 was smaller than that of Col-0. From these results, we speculated that root hair is one of the targets for Al attack and that the lower accumulation of Al ions in root region may be one of the Al resistant mechanisms in #355-2. These results also suggested that the Al influx is probably occurred not only in root-tip, but also in root-hair.
We constructed two types of transgenic lines, over-expressing and suppressing of the F9E10.5 gene and determined their Al sensitivity and root-hair formation, but we suggested that F9E10.5 may not relate to these phenotype.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Bunichi Ezaki et al.: "Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis"Plant Physiol.. 127. 918-927 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takayuki Sasaki et al.: "A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat"Plant Cell physiology. 43. 177-185 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mayandi Sivaguru et al.: "Aluminum-induced gene expression and protein localization of cell wall-associated receptor kinase in Arabidopsis"Plant Physiology. 132. 1-11 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takayuki Sasaki et al.: "A wheat gene encoding an aluminum-activated malate transporter"Plant Journal. 37. 645-653 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki et al.: "Mechanism of gene expression of Arabidopsis glutatione S-transferase, AtGST1 and AtGST11,in response to aluminum (Al) stress"Plant Physiology. 134. 1672-1682 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki, Hideaki Matsumoto: "Biotechnology for Sustainable Utilization of Biological Resources in the Tropics. Vol.15"Osaka university. 6 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki et al.: "Biotechnology in Sustainable Biodiversity and Food Security"Science Publishers, Inc.. 7 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideaki Matsumoto et al.: "Advances in Plant Physiology, Vol.5"Scientific Publishers (India), Jodhpur. 56 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki et al.: "Different mechanisms of four aluminum (Al)resistant transgenes for Al toxicity in Arabidopsis"Plant Physiology. 127. 918-927 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takayuki Sasaki et al.: "A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat"Plant Cell physiology. 43. 177-185 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mayandi Sivaguru et al.: "Aluminum-induced gene expression and protein localization of cell wall-associated receptor kinase in Arabidopsis"Plant Physiology. 132. 1-11 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takayuki Sasaki et al.: "A wheat gene encoding an aluminum-activated malate transporter"Plant Journal. 37. 645-653 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki et al.: "Mechanism of gene expression of Arabidopsis glutatione S-transferase, AtGST1 and AtGST11 in response to aluminum (Al) stress"Plant physiology (in press). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki, Hideaki Matsumoto: "Different mechanisms of four aluminum (Al) stress resistant transgenes for Al toxicity in Arabidopsis thaliana"Osaka university, Biotechnology for Sustainable Utilization of Biological Resources in the Tropics. Vol.15. 230-235 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bunichi Ezaki et al.: "Construction of aluminum (Al) stress-resistance transgenic Arabidopsis plants using Al-induced genes"Biotechnology in Sustainable Biodiversity and Food Security (Science Publishers, Inc.). 159-165

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideaki Matsumoto et al.: "Recent advances in the physiological and molecular mechanism of Al toxicity and tolerance in higher plants"Scientific Publishers, Advances in Plant Physiology. Vol.5. 29-74 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] B.Ezaki, M.Suzuki, H.Motoda, M.Kawamura, S.Nakashima, H.Matsumoto: "Mechanism of Gene expression of Arabidopsis Glutatione S-transferase, AtGST1 and AtGST11,in Response to Aluminum(Al)Stress"Plant Physiol. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Sivaguru, B.Ezaki, ZH.He, H.Tong, H.Osawa, F.Baluska, D.Volkmann, H.Matsumoto: "Aluminum-induced gene expression and protein localization of cell wall-associated receptor kinase in Arabidopsis"Plant Physiol. 43. 1-11 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Sasaki, Y.Yamamoto, B.Ezaki, M.Katsuhara, S.J, Ahn, P.Ryan, E.Delhaize, H.Matsumoto: "A wheat gene encoding an aluminum-activated malate transporter"Plant J.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Matsumoto, Y.Yamamoto, B.Ezaki: "Advances in Plant Physiology, Vol 5"Scientific Publishers, Jodhpup, India. 45 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takayuki Sasaki, Bunichi Ezaki, Hideaki Matsumoto: "A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat"Plant Cell Physiology. 43(2). 177-185 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Bunichi Ezaki, Richard C.Gardner, Yuki Ezaki, Masako Kawamura, Hideaki Matsumoto: "Construction of Aluminum (Al) stress-resistant transgenic Arabidopsis plants using Al-induced genes"Biotechnology in sustainable biodiversity and food security. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Bunichi Ezaki, Maki Katsuhara, Masako Kawamura, Hideaki Matsumoto: "Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis"Plant Physiology. 127. 918-927 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takayuki Sasaki, Bunichi Ezaki, Hideaki matsumoto: "A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat"Plant Cell Physiology. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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