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Regulation mechanism of Al-induced secretion of organic acid anions

Research Project

Project/Area Number 13660067
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKagawa University

Principal Investigator

MA jian feng  Kagawa University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80260389)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsAluminum / organic acid anion / metabolism / anion channel / root / secretion pattern / resistance / アルミニウム-シュウ酸錯体 / アルミニウム-クエン酸錯体 / アブシジン酸 / 分泌 / 耐性機構
Research Abstract

Secretion of organic acid anions from roots has been identified as a mechanism of resistance to Al. However, the process leading to the secretion of organic acid anions is poorly understood. We investigated the mechanism regulating Al-induced secretion of organic acid anions.
1. In buckwheat roots, ABA was found to be involved in the Al-induced secretion of oxalate. Only ABA rather than other phytohormone induced the secretion of exalate. When ABA synthesis was inhibited by Floridon, the Al-induced secretion of exalate was decreased. Al caused rapid increase in free ABA concentration in the root apices.
2. The effect of Al on the metabolism of organic acids was investigated in two lines of triticale (xTriticosecale Wittmark) differing in Al-induced secretion of malate and citrate and in Al resistance. Levels of citrate (room apices and mature root segments) and malate (mature segments only) acids in roots increased during exposure to Al, but similar changes were observed in both triticale genotypes. The in vitro activities of four enzymes involved in malate and citrate metabolism (citrate synthase, phosphoenol-pyruvate carboxylase, malate dehydrogenase and NADP-isocitrate dehydrogenase) were similar for sensitive and resistant lines in both root apices and mature root segments.
3. In barley (Hordeum vulgare L.), citrate was secreted from the roots in response to Al stress. A positive correlation between citrate secretion and Al resistance ((root elongation with Al) (root elongation without Al)) and a negative correlation between citrate secretion and Al content of root apices, were obtained, suggesting that citrate secretion from the root apices plays an important role in excluding Al and thereby detoxifying Al. The Al-induced secretion of citrate was characterized by a rapid, temperature-dependent, and no dose-response secretion.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Shen, R.F., Ma, J.F., Kyo, M., Iwashita: "Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench"Planta. 215. 394-398 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ma, J.F., Shen, R., Zhao, Z., Wissuwa, M, Takeuchi Y, Ebitani T, Yano: "Response of rice to Al stress and identification of quantitative trait loci for Al tolerance"Plant Cell Physiology. 43. 652-659 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Li, X.F., Ma, J.F., Matsumoto, H.: "Aluminum-induced secretion of both citrate and malate in rye"Plant and Soil. 242. 235-243 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 犬伏和之, 平館俊太郎, 櫻井克年, 川東正幸, 馬 建鋒, 谷昌幸, 岡崎正則, 林健太: "土壌酸性をめぐる研究の進展と今後の課題-レビューと課題の抽出-"日本土壌肥料学雑誌. 73. 575-583 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 阿江教治, 小林優, 桜井直樹, 白石友紀, 藤原徹, 馬建鋒, 間藤徹: "アポプラストの機能と無機元素"日本土壌肥料学雑誌. 73. 827-832 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen, R, Ma, J.F.: "Distribution and mobility of aluminium in an Al-accumulating plant, Fagonyrum esculentum Moench"J Expt Bot.. 52. 1683-1687 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen, R. And Ma, J. F.: "Distribution and mobility of aluminum in an Al-accumulating plant"Fagopyrum esculentum Moench. J. Exp. Bot.. 52. 1683-1687 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen, R. F., Ma, J. F., Kyo, M. and Iwashita, T.: "Compartmentation of aluminum in leaves of an Al-accumulator"Fagopyrum esculentum Moench. Planta. 215. 394-398 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ma, J. F., Shen, R., Zhao, Z., Wissuwa, M., Takeuchi, Y., Ebitani, T. and Yano, M.: "Response of rice to Al stress and identification of quantitative trait loci for Al tolerance"Plant Cell Physiol.. 43. 652-659 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Li, X. F., Ma, J. F. and Matsumoto, H.: "Aluminum-induced secretion of both citrate and malate in rye"Plant Soil. 242. 235-243 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Zhao, Z., Ma, J. F., Sato, K. and Takeda, K.: "Differential Al resistance and citrate secretion in barley (Hodeum vulgare L.)"Planta. 10.1007/_s00425-003-1043-2. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayes, J. and Ma, J. F.: "Al-induced efflux of organic acid anions is poorly associated with internal organic acid metabolism in triticale roots"J. Exp. Bot.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen, R.F., Ma, J F., Kyo, M., Iwashita, T.: "Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench"Planta. 215. 394-398 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ma, J F., Shen, R., Zhao, Z., Wissuwa, M., Takeuchi, Ebitani, T., Yano M.: "Response of rice to Al stress and identification of quantitative trait loci for Al tolerance"Plant Cell Physiology. 43. 652-659 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Li, X.F., Ma, J.F., Matsumoto, H.: "Aluminum-induced secretion of both citrate and malate in rye"Plant and Soil. 242. 235-243 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 犬伏 和之, 平館 俊太郎, 桜井 克年, 川東 正幸, 馬 建鋒, 谷 昌幸, 岡崎 正則, 林 健太郎, 坂: "土壌酸性をめぐる研究の進展と今後の課題-レビューと課題の抽出-"日本土壌肥料学雑誌. 73. 575-583 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿江 教治, 小林 優, 桜井 直樹, 白石 友紀, 藤原 徹, 馬 建鋒, 間藤 徹: "アポプラストの機能と無機元素"日本土壌肥料学雑誌. 73. 827-832 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shen, R, Ma, J.F.: "Distribution and mobility of aluminium in an Al-accumulating plant, Fagopyrum esculentum Moench"J Expt Bot.. 52. 1683-1687 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ma, J.F., Ryan, P.R., Delhaize, M.: "Aluminium tolerance in plants and the complexing role of organic acids"Trends in Plant Sci.. 6. 273-278 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ma, J.F., Zhang, W., Zhao, Z.: "Plant Nutrition-Food security and sustainability of agro-ecosysten through basic and applied Research"Kluwer Academic Publishers. 1043 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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