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Functional alteration and gene expression of enzymes for plant starch biosynthesis

Research Project

Project/Area Number 13660071
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ITO Hiroyuki  Hokkaido Univ., grad. School of Agr., Associate Prof., 大学院・農学研究科, 助教授 (10241366)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsAllosteric regulation / Transgenic plants / ADPglucose pyrophosphorylase / Starch biosynthesis / Mutated enzymes / Analysis of promoter region
Research Abstract

ADP-Glucose is a sole glucose donor for plant starch biosynthesis and is synthesized from glucose1-phosphate and ATP by ADPglucose pyrophsophorylase (AGPase ; EC 2.7.7.27). Plant AGPases are allosterically regulated by 3-phosphoglycerate and Pi. In plant leaves, the allosteric regulation of AGPases controls the rate of tansitory starch synthesis. The aim in this study was to extensively analyze the effect of allosteric properties on starch content in leaves and to enhance tha productivity by expression of allosterically mutated AGPase in plants.
1) The gene for mutated AGPase whose allosteric sensitivities are decreased was introduced Arabidopsis mutant plant (TL46) decreasing AGPase activity in leaves.
2) The AGPase activity in leaves of transgenic plants increased more than 10-and 1.5-fold of those of TL46 and wild-type plants, respectively.
3) The maximum starch level and turnover of starch in leaves of transgenic plants was about 1.3 and 1.8 times higher, respectively, than those in wild-type plants. Additionally, in transgenic plants the dry weight of leaves and leaf area were increased 1.8-fold of those in wild-type plants.
4) The total seed number of transgenic plants was about 1.3 times higher than that of wild-type plants.
These results show that the decreased sensitivity of AGPase for allosteric effectors generate the increase of starch levels, plant growth and yield, suggesting that the allosteric properties of AGPase not only regulate the rate of starch biosynthesis but influence plant growth and biomass.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] K.Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotechnol. Biochem. 65. 1141-1148 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Mizuno et al.: "Isolation and sequence of a putative α-glucosidase gene from Brevibacterium fuscum var. dextranlyticum strain 0407"J. Appl. Glycosci.. 48. 287-291 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] V.K.Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161. 467-476 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Kakuta et al.: "1-Aminocyclopropane-1-carboxylate synthase of Penicillium citrinum : primary structure and expression in Escherichia coli and Saccharomyces cerevisiae"Biosci. Biotech. Biochem.. 65. 1511-1518 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Hamada et al.: "Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359. 23-34 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70. 89-109 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.W.Okita et al.: "Increasing rice productivity and yield by manipulation of starch synthesis"Rice Biotechnology : Improving Yield, Stress Tolerance and Grain Quality. (John Wiley and Sons, New York). 18 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Hamada et al.: "Differential characteristics and subcellular localization of two starch-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L"J. Biol. Chem.. 277. 16538-16546 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] I.H.Kavakli et al.: "Generation, characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215. 430-439 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stages of development"Carbohydr. Res.. 338. 463-469 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 伊藤裕之, 松井博和: "Starch branching enzymeの酵素特性と細胞内局在性"日本農芸化学会誌(ミニレビュー). 77(4). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N.Isono et al.: "Molecular cloning of a cDNA encoding granule-bound starch synthase I from kidney bean seeds and expression of Escherichia coli"J. Appl. Glycosci.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] D.Omoto et al.: "Isolation and characterization of two cDNAs for large and small subunits of ADP-glucose pyrophosphorylase from kidney bean"J. Appl. Glycosci.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotech. Biochem.. 65(5). 1141-1148 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Mizuno et al.: "Isolation and sequence of a putative α-glucosidase gene from Brevibacterium fuscom var. dextranlyticum strain 0407"J. Appl. Glycosci.. 48(3). 287-291 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] V.K. Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161(3). 467-476 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Kakuta et al.: "1-Aminocyclopropane-1-carboxylate synthase of Penicillium citrinum : Primary structure and expression in Escherichia coil and Saccharomyces cerevisiae"Biosci. Biotech. Biochem.. 65(7). 1511-1518 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Hamada et al.: "Two starch-branching enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359(1). 23-34 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70(2). 89-109 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Hamada et al.: "Differential characteristics and subcellular localization of two strach-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L"J. Biol. Chem.. 277(19). 16538-16546 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] I.H. Kavakli et al.: "Generation , characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215(3). 430-439 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stages of development"Carbohydr. Res.. 338(5). 463-469 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N. Isono et al.: "Molecular cloning of a cDNA encoding granulebound starch synthase I from kidney bean seeds and expression in Escherichia coil"J. Appl. Glycosci.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Ito, H. Matsui: "Enzymatic properties and subcellular localization of starch branching enzymes from Phaseolus vulgaris L"Nippon Nogelkagaku Kaishi (Mini Review ; Japanese). in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.W. Okita et al., eds. By J. A. Goode and D. Chadwick: "Increasing rice productivity and yield by manipulation of starch synthesis, Rice Biotechnology : Improving Yield, Stress Tolerance and Grain Quality"John Wiley and Sons.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70(2). 89-109 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Hamada et al.: "Differential characteristics and subcellular localization of two starch-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L."J. Biol. Chem.. 277(19). 16538-16546 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] I.H.Kavakli et al.: "Generation, characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215(3). 430-439 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stage of development"Carbohydr. Res.. 338(5). 463-469 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Isono et al.: "Molecular cloning of a cDNA encoding granule-bound starch synthase l from kidney bean seeds and expression in Escherichia coli"J. Appl. Glycosci.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 伊藤浩之, 松井博和: "Starch branching enzymeの酵素特性と細胞内局在性"日本農芸化学会誌. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] K. Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotech. Biochem.. 65(5). 1141-1148 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] V. K. Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161(3). 467-476 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S. Hamada et al.: "Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359(1). 23-34 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] I. H. Kavakli et al.: "Generation, characterization, and heterologous expression of up-regulated Arabidopsis thaliana leaf ADP-glucose pyrophosphorylases"Planta. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T. W. Okita et al.: "Increasing rice productivity and yield by manipulation of starch synthesis"John Wiley and Sons, New york. 18 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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