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Study of the mechanism of uneven distrlbudon of leucocytolytic toxin in Staphylococcus aureus-Phage conversion and regulation of PVL expression-

Research Project

Project/Area Number 13660075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionTohoku University

Principal Investigator

KANEKO Jun  TOHOKU University, Graduate School of Agricultural Science, Assistant professor, 大学院・農学研究科, 助手 (30221188)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsStaphylococcus aureus / Panton -Valentine Leukocidin / Phage conversion / regulation / Panton -Valentine Leukocidim
Research Abstract

Staphylococcus aureus is known as community-accuired, nosocominal pathogen. It produces bi-component toxin, called Panton-Valentine leukocidin (PVL) which acts to polymorphonuclear lukocytes and macrophages. The epidemiological studies of PVL shows that PVL producing strains were isolated from limited clinical samples, most of cotenous necrotic lesions, such as furuncles, however, PVL cluster was seen only few % of clinical isolated strain while almost all S. aureus has hlg/luk cluster. The aim of this study is clarification of the mechanism of these uneven distribution of PVL producing strains among the clinical isolated S. aureus.
(1) Phage conversion of PVLP Previously, we found that gene cluster of two components for PVL are encoded in the prophages φPVL and φSLT. Therefore we assumed that phage conversion of PVL is related to the uneven distribution of PVL gemes. In this *y, we isolated 10 novel PVL-carrying phages and whole genome sequence of three of them were determined. We showed that these PVL carrying phages are divided into two groups, φPVL and φSLT types. In addition, each type phage divided into several subtypes by difference of lysogenic and replication regions. These data shows that PVL genes are carried by several different temperate phases and these deference were reflected to host ranges of PVL converting phages. We tested 257 S. aureus isolates from food poisoning origins, and only few percent of strains were infected by these phages.
(2) Regulation of PVL expression. Regulation of expression of PVL is important factor for the infection of S. aureus in the high-level PVL producer related lesions. We found several indicator strains which could not produce PVL components when PVL genes were transduced by φSLT. Now we are studying about the effect of the well known global regulators, such as agr and sigB to the expression of PVL.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] S.Narita: "Phage conversion of Panton-Valentineleukocidin (PVL) in Staphylococcus aureus : molecular analysis of a PVL-converting phage, φ SLT"Gene. 268(1-2). 195-206 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 金子 淳: "ブドウ球菌の2成分性崩壊毒素のフアージ変換"日本農芸化学会誌. 79(9). 939-947 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 金子 淳: "黄色ブドウ球菌の2成分性膜孔形成毒素の構造及び細胞崩壊機構"蛋白質核酸酵素. 46(4). 497-505 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Nishiyama: "Identification of serine138 residue in the 4-residue segment K135K1361137S138 of LukS-I component of Staphylococcus intermedius leukocidin crucial for the LukS-I-specific function of staphylococcal leukocidin"Biosci.Biotechnol.Biochem. 66(2). 328-335 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 神尾好是, 冨田敏夫, 金子淳: "細菌毒素ハンドブツク(分担執筆.13章1-5α-、β-、δ-、γ-毒素、ロイコシジン.計31ページ)"サイエンスフォーラム. 527 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Narita, J.Kaneko, J.Chiba, Y.Piemont, S.Jarraud, J.Etienne, Y.Kamio: "Phage conversion of Panton-VaIentine leukocidin (PVL) in Staphylococcus aureus : molecular analysis of a PVL, converting phage, : SLT."Gene. 268(1-2). 195-206 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J.Kaneko: "Phage conversion of bi-component cytotoxin in Staphylococcus aureus : (in Japanese)"Nippon Nougeikagaku kaishi. 75(9). 939-947 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J.Kaneko, T.Tomita, Y.Kamio: "Structure and mode of action of staphylococcal bi-component pore-forming toxin (in Japanese)"Tanpakushitu kakusan kouso. 46(4). 497-505 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Nishiyama, Marie Antonette Ruth V, Guerra, N.Sugawara, K.Yokota, J.kaneko, Y.Kamio: "Identification of serinel38 residue in the 4-residue segment K135K1361137S138 of LukS-1 component of Staphylococcus intermedius leuokocidin crucial for the LukS-1-specific function of staphylococcal leukocidin"Biosci. Biotechnol. Biochem.. 66(2). 328-335 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Kamio, T.Tomita, and J.Kaneko: "Pore-Forming cytolysins from Staphylococcus aureui, α-and γ-hemolysins, and leukocidin"In : Staphylococcal Infection and Immunity. ed. A.,L.,Honeyman, H.,Friedman, M.,Bendinelli, Kluwer Academic/Plenum Publishers. 179-212 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Kamio, T.Tomita, and J.Kaneko: "α-, β-, δ-,γ-hemolysins, and leukocidin from Staphylococcus aureus (in Japanese)"In : A handbook of bacterial toxins. ed.J.,Sakurai, T.,Honda, K.,Oguma, Science Forum. 214-244 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 神尾好是, 冨田敏夫, 金子淳: "細菌毒素ハンドブック(分担執筆.13章1-5、31ページ)"サイエンスフォーラム. 527 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Narita, J.Kaneko, J.Chiba, Y.Pienont, S.Jarraud, J.Etienne, Y.Kamio: "Phage conversion of Panton-Valentineleukocidin (PVL) in Staphylococcus aureus : molecular analysis of a PVL-converting phage, φSLT"Gene. 268・1-2. 195-206 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 金子 淳: "ブドウ球菌の2成分性崩壊毒素のファージ変換"日本農芸化学会誌. 75・9. 939-947 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 金子 淳、冨田 敏夫、神尾 好是: "黄色ブドウ球菌の2成分性膜孔形成毒素の構造及び細胞崩壊機構"蛋白質核酸酵素. 46・4. 497-505 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Nishiyama, Marie Antonette Ruth V, Guerra, N.Sugawara, K.Yokota, J.Kaneko, Y, Kamio: "Identification of serine138 residue in the 4-residue segment K135K136T137S138 of LukS-I component of Staphylococcus intermedius leukocidin crucial for the LukS-I-specific function of staphylococcal leukocidin"Biosci. Biotechnol. Biochem.. 66・2. 328-335 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Kamio, T.Tomita, and J.Kaneko: "Staphylococcal Infection and Immunity"Pore-Forming cytolysins from Staphylococcus aureus, α- and γ- hemolysins, and leukocidin. 330 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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