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Molecular mechanisms of transcriptional repression through chromatin structure : Approaches from the actin-related protein Arp6

Research Project

Project/Area Number 13660076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionTohoku University

Principal Investigator

HARATA Masahiko  TOHOKU UNIVERSITY, GRAD SCH.AGRI SCI, ASSOCIATE PROFESSOR, 大学院・農学研究科, 助教授 (70218642)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsactin-related protein / chromatin / transcription alregulation / chromatin-remodeling complex / transcriptional co-repressor / cell nucleus / protein complex / heterochromatin / 遺伝子発現抑制 / クロマチンリモデリング / ヘテロクロマチシ
Research Abstract

Actin plays central roles in the organization and dynamics of the cytosketeton. Within a few decades of the first isolation of actin from muscle, it was shown that actin filaments form the main architecture of the cytoskeleton, and that the dynamics of the cytoskeleton are regulated by the assembly/disassembly of the filament. Because of these characteristics of actin, previous researchers hypothesized that actin and/or its evolutionarily related molecules were involved in the organization and dynamics of the nucleus. However, actin filaments were observed only in the cytoplasm, and no molecule evolutionarily related to actin was identified at the time. The hypothesis was therefore regarded with skepticism for a long time. When the subcellular localizations of the other yeast Arps were investigated, unexpectedly more than half of them, including Arp5, Arp6, Arp7, Arp8, and Arp9, were predominantly localized in the nucleus, and Arp1, Arp2, Arp3, and Arp10 were observed in the cytoplasm. This suggests that the roles of Arps in the nucleus are not less significant than those in the cytoplasm. We have also discovered Arps localized in the nucleus in mammals. One of the nuclear Arps, Arp6 of budding yeast was shown to be a component of Swr1 complex, which exchange histone H2A for its variant H2A.Z. We showed that Arp6 was present in chromatin regions adjacent to telomere, and was required for proper organization of heterochromatin and distribution of Sir3. We also showed that human hArp6 interacted with hTPR1, which shuttles between cell nucleus and the cytoplasm. The property of hTPR1 might be involved in the regulation of Arp6 function. We have partially purified complexes containing hArp6, and analyzed it. Apart of these researches were performed in the laboratory of Prof.Ulrike Wintersberger.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (38 results)

All 2003 2002 2001 Other

All Journal Article (18 results) Publications (20 results)

  • [Journal Article] The brain-specific actin-related protein ArpNα interacts with the transcriptional co-repressor CtBP2003

    • Author(s)
      Oma, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 301

      Pages: 521-528

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The brain-specific actin-related protein ArpNα interacts with the transcriptional co-repressor CtBP2003

    • Author(s)
      Oma, Y., Nishimori, K., Harata, M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 301

      Pages: 521-528

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Brain-specific expression of the nuclear actin-related protein ArpNα and its involvement in mammalian SWI/SNF chromatin remodeling complex2002

    • Author(s)
      Kuroda, Y
    • Journal Title

      Biochem.Biophys.Res.Commun 299

      Pages: 328-334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae2002

    • Author(s)
      Harata, M.
    • Journal Title

      Nucleic Acids Res 30

      Pages: 1743-1750

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Alternative splicing Products of the gene for a human nuclear actin-related protein, hArpNβ/Baf53,encode a protein isoform, hArpNβS, localized in the cytoplasm2002

    • Author(s)
      Ohfuchi, E
    • Journal Title

      Biosci.Biotechnol.Biochem 66

      Pages: 1740-1743

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Z and W chromosomes of chickens : studies on their gene functions in sex detrmination and sex differentiation2002

    • Author(s)
      Mizuno S.
    • Journal Title

      Cytogenet.Genome Res 99

      Pages: 236-244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Alternative splicing products of the gene for a human nuclear actin-related protein, hArpNβ/Baf53, encode a protein isoform, hArpNβS, localized in the cytoplasm2002

    • Author(s)
      Ohfuchi, E., Nishimori, K., Harata, M.
    • Journal Title

      Biosci.Biotechnol.Biochem. 66

      Pages: 1740-1743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae2002

    • Author(s)
      Harata, M., Zhang, Y., Stillman, D.J., Matsui, D., Oma, Y., Nishimori, K., Mochizuki, R.
    • Journal Title

      Nucleic Acids Res. 30

      Pages: 1743-1750

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Brain-specific expression of the nuclear actin-related protein ArpNα and its involvement in mammalian SWI/SNF chromatin remodeling complex2002

    • Author(s)
      Kuroda, Y., Oma, Y., Nishimori, K., Ohta, T., Harata, M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 299

      Pages: 328-334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Z and W chromosomes of chickens : studies on their gene functions in sex determination and sex differentiation2002

    • Author(s)
      Mizuno, S., Kunita, R., Nakabayashi, O., Kuroda, Y., Arai, N., Harata, M., Ogawa, A., Itoh, Y., Teranishi, M., Hori, T.
    • Journal Title

      Cytogenet.Genome Res. 99

      Pages: 236-244

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Identification of two cDNAs for human actin-related proteins (Arps) that hae remarkable similarity to conventional actin2001

    • Author(s)
      Harata, M
    • Journal Title

      Biochim.Biophys.Acta 1522

      Pages: 130-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Absence of Z-chromosome inactivation for five genes in male chickens2001

    • Author(s)
      Kuroda, Y
    • Journal Title

      Chromosome Res 9

      Pages: 457-467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Novel actin-related proteins in vertebrates : similarities of structure and expression pattern to Arp6 localized on Drosophila heterochromatin2001

    • Author(s)
      Kato, M
    • Journal Title

      Gene 268

      Pages: 133-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Co-localization of chicken DNA topoisomeraseIIα, but notβ, with sites of DNA replication and possible nvolvement of a C-terminal region of α through its binding to PONA2001

    • Author(s)
      Niimi, A.
    • Journal Title

      Chromosoma (Berl.) 110

      Pages: 102-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Novel actin-related proteins in vertebrates : similarities of structure and expression pattern to Arp6 localized on Drosophila heterochromatin2001

    • Author(s)
      Kato, M., Sasaki, S., Mizuno, S., Harata, M.
    • Journal Title

      Gene 268

      Pages: 133-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Co-localization of chicken DNA topoisomerasellα, but not β, with sites of DNA replication and possible involvement of a C-terminal region of α through its binding to PCNA2001

    • Author(s)
      Niimi, A., Suka, N., Harata, M., Kikuchi, A., Mizuno, S.
    • Journal Title

      Chromosoma(Berl.) 110

      Pages: 102-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Absence of Z-chromosome inactivation for five genes in male chickens2001

    • Author(s)
      Kuroda, Y., Arai, N., Arita, M., Teranishi, M., Hori, T., Harata, M., Mizuno, S.
    • Journal Title

      Chromosome Res. 9

      Pages: 457-467

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Identification of two cDNAs for human actin-related proteins (Arps) that have remarkable similarity to conventional actin2001

    • Author(s)
      Harata, M., Nishimori, K., Hatta, S.
    • Journal Title

      Biochim.Biophys.Acta. 1522

      Pages: 130-133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oma Y.et al.: "The brain-specific actin-related protein ArpNa interacts with the transcriptional co-repressor CtBP"Biochem.Biophys.Res.Commun.. 301. 521-528 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田昌彦: "細胞核におけるアクチンファミリーの多様な機能"蛋白質 核酸 酵素. 49. 543-552 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Osada, M.et al.: "Molecular cloning and expression analysis of vitellogenin in scallop, Patinopecten yessoensis(Bivalvia, Mollusca)"Mol.Reprod.Dev.. 67. 273-281 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno, M.et al.: "Fission yeast Arp6 is required for telomere silencing but functions in dependently of Swi6"Nucleic Acids Res.. 32. 736-741 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田昌彦: "クロマチン構造変換複合体によるゲノム機能の制御:アクチン関連タンパク質(Arp)からのアプローチ"日本農芸化学会誌. 78(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田昌彦, 尾間由佳子 (分担): "細胞核のダイナミクス 核内アクチンファミリーの分子機能"シュプリンガー・フェアラーク東京(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Harata, M: "Nuclear Dynamics : Approaches from Biochemistry, Molecular Cell Biology, and Visual Biology"Springer-Verlag Tokyo, Inc.(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田昌彦(分担執筆): "応用生命科学のための生物学入門[改訂版]"倍風館. 270 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuroda Y. et al.: "Brain-specific expression of the nuclear actin-related protein ArpNalpha and its involvement in mammalian SWI/SNF chromatin remodeling complex"Biochem. Biophys. Res. Commun.. 299. 328-334 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohfuchi E. et al.: "Alternative splicing products of the gene for a human nuclear actin-related protein, hArpNbeta/Baf53, encode a protein isoform, hArpNbetaS, localized in the cytoplasm"Biosci. Biotechnol. Biochem.. 66. 1740-1743 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harata M. et al.: "Correlation between chromatin association and transcrptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae"Nucleic Acids Res.. 30. 1743-1750 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原田昌彦, 尾間由佳子: "クロマチン機能ドメインの形成-アクチン関連タンパク質の関与"実験医学. 20. 1601-1608 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原田昌彦: "核マトリックスによる核構築-アクチンファミリーからのアプローチ"細胞工学. 21. 1164-1168 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oma Y.et al.: "The brain-specific actin-related protein ArpNalpha interacts with the transcriptional co-repressor CtBP"Biochem. Biophys. Res. Commun.. 301. 521-528 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kuroda, Y. et al.: "Absence of Z-chromosome inactivation for five genes in male chickens"Chromosome Res.. 9. 457-467 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kato, M. et al.: "Novel actin-related proteins in vertebrates : similarities of structure and expression pattern to Arp6 localized on Drosophila heterochromatin"Gene. 268. 133-140 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Niimi, A. et al.: "Co-localization of chicken DNA topoisomeraseIIα, but not β, with sites of DNA replication and possible involvement of a C-terminal region of α through its binding to PCNA"Chromosoma (Berl.). 110. 102-114 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, H. et al.: "The N-terminal internal region of BLM is required for the formation of dots/rod-like structures which are associated with SUMO-1"Biochem. Biophys. Res. Commun.. 286. 322-327 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Harata, M.et al.: "Identification of two cDNAs for human actin-related proteins (Arps) that have remarkable similarity to conventional actin"Biochim. Biophys. Acta.. 1522. 130-133 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Harata, M. et al.: "correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae"Nucl. Acids Res.. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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