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Molecular Mechanisms of Self-defense system in Insect

Research Project

Project/Area Number 13660093
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionTottori University

Principal Investigator

MORISHIMA Isao  Tottori University, Faculty of Agriculture,Professor, 農学部, 教授 (30032296)

Co-Investigator(Kenkyū-buntansha) YAMANO Yoshiaki  Tottori University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (00182593)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsinsect immunity / Antibacterial protein / Peptidglycan recognition protein / Attacin / Lysozyme / Samia cynthia ricini / Bombyx mori / cDNAクローニング / ペプチドグリカン
Research Abstract

1. Screening of bacteria-induced genes in Eri-silkworm, Samia cynthia ricini
Comparing mRNAs in the fat bodies from naive and bacteria-injected larvae by means differential cloning method,12 DNA fragments,which were specific for bacteria-injected larvae,were isolated.After screened a cDNA library of immunized Samia fat body with these DNA fragments as probes,cDNAs for a lysozyme,three attacins and two peptidoglycan recognition proteins(PGRP)have been cloned and sequenced.
2.Isolation and characterization of lysozyme and attacin.
A lysozyme was isolated from immunized hemolymph of S.ricini.The N-terminal amino acid sequence was in perfect match of that deduced from the cDNA.The properties of the enzyme was compared with other lepidopteran lysozyme.An attacin was similarly isolated and characterized.The attacin was bactericidal only to Gram-negatives.
3.Isolation and characterization of peptidoglycan-binding proteins from Bombyx mori and S.ricini.
Detecting peptidoglycan (PGN) binding activity by means of photo affinity labeling with ^<125>I-PGN,three proteins,which were different from PGRP,were isolated and characterized from the hemolymph of B.mori.One of the proteins (31kd) was the hemolymph protein (C-19),which had been isolated and sequenced,but whose physiological role was not known.The protein binds not only to PGN but oligo chitin and LPS. The second protein was promoting protein,which had been isolated as the protein stimulated replication of nucleopolyhedrovirus.Two PGRP were isolated from immunized hemolymph of Samia larvae.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] K.Kishimoto et al.: "Protein purification, cDNA cloning and gene expression of attacin, an antibacterial protein, from eri-silkworm, Samia cynthia ricini"Insect Biochem. Molec. Biol.. 32. 881-887 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Fujimoto et al.: "Protein purification cDNA cloning and gene expression of lysozyme from eri-silkworm, Samia cynthia ricini"Comp. Biochem. Physiol.. 128B. 709-718 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 森嶋 伊佐夫: "エリ蚕の抗菌性タンパク質"野蚕. 47. 3-5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujimoto,S., et al.: "Protein purification, cDNA cloning and gene expression of lysozyme from eri-silkworm, Samia cynthia ricini"Comp. Biochem. Pysiol.B. 128. 709-718 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kishimoto,K., et al.: "Protein purification, cDNA cloning and gene expression of attacin, an antibacterial protein, from eri-silkworm, Samia cinthia ricini"Insect Biochem. Molec. Biol. 32. 881-887 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishima,I.: "Antibacterial proteins in Eri-silkworm"Wild Silkworm News. 47. 3-5 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Kishimoto et al.: "Protein purification, cDNA cloning and gene expression of attacin, an antibacterial protein, from eri-silkworm, Samia cynthia ricini"Insect Biochem. Molec. Biol.. 32. 881-887 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Fujimoto et al.: "Protein purification, cDNA cloning and gene expression of lysozyme from eri-silkworm Samia cynthia ricini"Comp. Biochem. Physiol.. 128B. 709-718 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Fujimoto et al.: "Protein purification, cDNA cloning and gene expression of lysozyme from eri-silkworm, Samia cynthia ricini"Comp. Biochem. Physiol. 128B. 709-718 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Kishimoto et al.: "Protein purification, cDNA cloning and gene expression of attacin, an antibacterial protein, from eri-silkworm, Samia cynthia ricini"Insect Biochem. Molec. Biol.. 32(印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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