Screening of the naturally nematicidal compounds against plant parasitic nematode Pratylenchus penetrans Mechanisms

• Project/Area Number: 13660107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tottori University
• Principal Investigator: KIMURA Yasuo Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (10011983)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: nematicide / nematode / plant parasite / natural compound / fungus / 植物寄生性線虫 / α、β-dehydrocurvularin / terrestric acid / 植物毒 / 微生物 / Aspergillus sp.

Research Abstract

In the course of a screening search for new nematicidal compounds among the fungal metabolites, using bioassay method with a root-lesion nematode Pratylenchus penetrans, suitable for developing new nematicides and new lead compounds, I found the presence of active compounds in the metabolites of Penicillium bilaiae Chalabuda, Penicillium cf. simplicissimum, and an unidentified Aspergillus sp.
Biosassay-guided fractionation led to the isolation of four active compounds,2,6-pyridinedicauboxylic acid,6-methoxypicolinic acid, p-hydroxyacetophenone and a new compound named pratynolene from the culture filtraste of Penicillium bilaiae Chalabuda. Three active compounds together with a related but inactive compound were also isolated from the mycelial mats of Penicillium cf.simplicissimum. Four curvuralin analogs were obtained from the culture filtrate and mycelial mats,although two of them were inactive.
From these results together with bioassays with rice and lettuce seedlings,the following facts became clear.
1) Active compounds are present in the culture filtrate or mycelial mats.
2) Some of the nematicidal compounds shows phytotoxic activity.
3) Some compounds have selectivity such as a compound shows nematicidal activity against P.penatrans but sit is inactive against C. elegance.

Research Products

• [Publications] Kusano, M.: "βγ-Dehydrocurvuralin and related compounds as nematicides of Pratylenchus penetrans from the fungus Aspergillus sp."Biosci. Biotechnol. Biochem. 67. 1413-1416 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kimura, Y.: "Nematicidal compounds from the fungi"Abstract of 3^<rd> Pan Pacific Conference. (in press). (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kusano,M., Nakagami,K., Fujioka,S., Kawano,T., Shimada,A., Kimura,Y.: "βγ-Dehydrocurvularin and reloated compounds as nematicide of Pratylenchus penetrans from the fungus Asergillus sp"Biosci.Biotech.Boichem.. 67. 1413-1416 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kimura,Y., Kusano,M., Nakahara,S.: "Nematicidal compounds from the fungi"Abstract of 3^<rd>Pan Pacific Pestiside Science, (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M.Kusano, Y.Kimura et al.: "β、γ-Dehydrocurvuralin and related compounds as nematicides of Pratylencus penetrans from the fungus Aspergillus sp."Bioscience, Biotechnology and Biochemistry. 66(in press). (2003)
• [Publications] Kusano, M., Nakagami, K., Fujioka, S., Kawano, T., Kimura, Y.: "Staraucture and nematicidal activity of three macrocyclic lactones produced by the fungus, Aspergillus, sp. No. K55-2"Tetrahedron.