Immunosuppressive effects induced by oral administration of peptide fragments derived from bovine β -lactoglobulin
| Project/Area Number |
13660117
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
食品科学・栄養科学
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
ENOMOTO Atsushi Gunma University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (70183217)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIZUMACHI Koko National Institute of Livestock and Grassland Science, Department of Animal Products Research, Head of Functional Biomolecules Laboratory, 品質開発部, 室長
KURISAKI Jun-ichi National Institute of Agrobiological Sciences, Genetic Diversity Department, Director, 遺伝資源研究グループ, グループ長
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | Oral tolerance / T cell response / Antibody response / Epitope / Class and subclass / Cytokine / Peptide / Cow's milk allergy / 経口寛容 / 牛乳アレルゲン / アレルゲン / ELISA / β-ラクトグロブリン |
|---|
| Research Abstract |
It has been well documented that oral administration of protein antigens commonly induces systemic immunological unresponsiveness to the subsequent parenteral immunization which is termed oral tolerance. In this study, bovine β -lactoglobulin ( β -Lg), one of the most potent cow's milk allergens, was selected as a target protein antigen, and groups of 5 female C3H/HeN mice were orally given with 3 mg of synthetic peptides 91-104 or 129-143 derived from β -Lg 5 times during 10 days prior to intraperitoneal injections with the whole antigen. Using this experimental system. we examined and compared the magnitude and epitope specificity of immunological tolerance observed in each group of the mice. All the feeding peptides have been shown to include both the immunodominant T and B cell determinants of the native protein. The oral administration of the peptides 91-104 or 129-143 was usually found to lower the production of anti- β -Lg serum antibody to approximately 1/2, as compared with the PBS-administrated control mice. Interestingly, detailed epitope-mapping study of antisera pooled for each group with the multi-pin peptide synthesis system indicates that the oral tolerance with the peptide fragments used in this study may not be directed to the antibody response against the corresponding epitopes on the protein antigen, but be capable of preferentially inhibiting the response to distinct determinants which are separate from the feeding peptides. In the case of T cell response, the orally given peptides, however, could mainly suppress the response against the antigenic determinant located in the peptides.
|
Report
(3 results)
Research Products
(6 results)
